FastQCFastQC Report
Thu 2 Feb 2017
SRR4062180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242265
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.11865423239002951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20550.022.5990831
TTAGGAC3400.021.9984913
GGTATCA10500.021.5907691
TAGGACG3900.021.4344274
GTCCTAC5250.019.2849581
GTGTACG750.001286967817.6080061
GACGTGA2750.017.5987937
TCCTACA6550.017.4658572
ATACCGT2900.017.4470796
GTAGGAC5150.017.0862063
ACCGTCG2850.016.981298
AACGCAG28050.016.7831616
AATCACG2500.016.72019834
AGGACGT5000.016.7188535
GGTCTAA1206.3791776E-616.5075051
TACCGTC2950.016.4056537
GTATAGC2301.8189894E-1216.2682671
TATGGCG5450.016.14568116
CGCAATA1501.8382889E-716.13287536
ATCAACG29400.016.0873323
Produced by FastQC (version 0.11.5)