##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062180_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1242265
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287437060530564	32.0	32.0	32.0	32.0	32.0
2	31.48929254225145	32.0	32.0	32.0	32.0	32.0
3	31.58958354296386	32.0	32.0	32.0	32.0	32.0
4	31.6681022165158	32.0	32.0	32.0	32.0	32.0
5	31.650474737676745	32.0	32.0	32.0	32.0	32.0
6	35.293825391522745	36.0	36.0	36.0	36.0	36.0
7	35.30029180569363	36.0	36.0	36.0	36.0	36.0
8	35.25782502123138	36.0	36.0	36.0	36.0	36.0
9	35.31966367884469	36.0	36.0	36.0	36.0	36.0
10	35.22225451091353	36.0	36.0	36.0	36.0	36.0
11	35.33165548413583	36.0	36.0	36.0	36.0	36.0
12	35.25822429191839	36.0	36.0	36.0	36.0	36.0
13	35.28481443170338	36.0	36.0	36.0	36.0	36.0
14	35.25490052444527	36.0	36.0	36.0	36.0	36.0
15	35.23812149581611	36.0	36.0	36.0	36.0	36.0
16	35.24281051144482	36.0	36.0	36.0	36.0	36.0
17	35.22814455852817	36.0	36.0	36.0	36.0	36.0
18	35.22652413132464	36.0	36.0	36.0	36.0	36.0
19	35.213208936901545	36.0	36.0	36.0	36.0	36.0
20	35.205222718180096	36.0	36.0	36.0	36.0	36.0
21	35.19311580057395	36.0	36.0	36.0	36.0	36.0
22	35.17619348528696	36.0	36.0	36.0	36.0	36.0
23	35.15108129102889	36.0	36.0	36.0	36.0	36.0
24	35.13612473989044	36.0	36.0	36.0	36.0	36.0
25	35.122674308621754	36.0	36.0	36.0	36.0	36.0
26	35.08771638901523	36.0	36.0	36.0	36.0	36.0
27	35.06875425130709	36.0	36.0	36.0	36.0	36.0
28	35.04458951994945	36.0	36.0	36.0	36.0	36.0
29	35.02279223837104	36.0	36.0	36.0	36.0	36.0
30	35.00432999400289	36.0	36.0	36.0	36.0	36.0
31	35.00520903349929	36.0	36.0	36.0	36.0	36.0
32	34.974415281763555	36.0	36.0	36.0	36.0	36.0
33	34.958492753156534	36.0	36.0	36.0	36.0	36.0
34	34.94117921699476	36.0	36.0	36.0	36.0	36.0
35	34.923977573223105	36.0	36.0	36.0	36.0	36.0
36	34.9017206473659	36.0	36.0	36.0	36.0	36.0
37	34.87541627591536	36.0	36.0	36.0	32.0	36.0
38	34.86449751059556	36.0	36.0	36.0	32.0	36.0
39	34.82903406278049	36.0	36.0	36.0	32.0	36.0
40	34.82389264770399	36.0	36.0	36.0	32.0	36.0
41	34.79477084197011	36.0	36.0	36.0	32.0	36.0
42	34.762687510313825	36.0	36.0	36.0	32.0	36.0
43	34.75649559474025	36.0	36.0	36.0	32.0	36.0
44	34.69947072484534	36.0	36.0	36.0	32.0	36.0
45	34.679359878930825	36.0	36.0	36.0	32.0	36.0
46	34.63552221144442	36.0	36.0	36.0	32.0	36.0
47	34.605851408515896	36.0	36.0	36.0	32.0	36.0
48	34.56958458943945	36.0	36.0	36.0	32.0	36.0
49	34.541927044551684	36.0	36.0	36.0	32.0	36.0
50	34.15696892369986	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	3.0
20	17.0
21	33.0
22	86.0
23	287.0
24	638.0
25	1539.0
26	3066.0
27	5843.0
28	10287.0
29	15936.0
30	23284.0
31	33950.0
32	50745.0
33	84121.0
34	189296.0
35	823132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67201698746313	17.94135717719512	12.331693584688091	26.054932250653657
2	15.706531058128181	20.047689467824505	38.01560614488936	26.230173329157957
3	18.713443701892192	23.677774897726888	28.68122827551676	28.927553124864158
4	12.629028427911921	15.994654924673881	35.74591572651568	35.630400920898516
5	14.281654880399916	36.87184296426286	33.5229600769562	15.323542078381022
6	34.27846483146456	35.26135007055683	16.724987744121407	13.7351973538572
7	30.134794105927483	30.55233786671926	20.31748459467183	18.995383432681432
8	27.751647192829225	33.58164320817217	19.144868445943498	19.521841153055107
9	27.14203491203568	14.526691164928579	18.814584649813042	39.5166892732227
10	16.109780207749722	26.982831346638076	31.397593426196043	25.509795019416163
11	36.413084164811856	21.53002781210128	22.71479917730919	19.34208884577767
12	24.62689090241688	23.58363439655339	29.082546061062704	22.70692863996703
13	29.370786426406603	20.018192575658173	25.388705308448678	25.222315689486543
14	23.290360752335452	19.802779600165827	25.66658482690891	31.240274820589807
15	24.838903132584434	27.684672755007988	22.52659456718172	24.949829545225857
16	24.937271434171834	26.226135171707625	24.47722170211156	24.35937169200898
17	23.555682563704202	26.230071683577982	25.73661819338064	24.47762755933718
18	23.962439576096887	25.500758694803444	26.93032485017287	23.6064768789268
19	25.201949664524076	25.045541812737216	26.072939348689694	23.679569174049014
20	25.51728135082398	24.722361752413335	25.81520514158021	23.945151755182472
21	25.71055749824514	24.56369982547993	25.45771269231017	24.268029983964762
22	25.284299243720138	24.674767461048972	25.846337134186342	24.194596161044544
23	24.186565818537765	24.768889704603854	26.02385644275291	25.020688034105472
24	24.26053650950049	25.005956923393963	26.278726055582922	24.45478051152262
25	24.429529012777486	24.840150596129806	26.200571054599546	24.529749336493158
26	23.86956031748587	25.518088000901585	26.533817397324235	24.078534284288313
27	24.388855795083156	25.240207363997875	25.92669811472639	24.444238726192584
28	24.17743946664477	25.141014402104574	26.061763784877268	24.61978234637339
29	24.133253800932334	25.189070573736117	25.9928469138128	24.684828711518755
30	24.201472322510273	25.33376802669339	26.110259165791245	24.354500485005094
31	24.733852225901	24.86828452313966	25.708285134927362	24.689578116031978
32	24.392637266392754	25.351859842093738	25.678765085457428	24.57673780605608
33	24.142430616432456	25.080075765076742	26.11512395543288	24.662369663057923
34	24.567175449004907	25.16528516470557	26.139487557627426	24.128051828662095
35	24.761080611144205	25.032199378551994	26.17334535443466	24.033374655869142
36	24.132429390901436	25.400438876823085	26.037663552455122	24.42946817982036
37	24.82052437954324	25.16079952987772	25.551628925400287	24.46704716517875
38	24.33168446345989	25.22368415756702	25.892060067698917	24.552571311274164
39	24.896116253197818	25.095994860954363	25.56425658520721	24.443632300640612
40	25.106660098014594	25.166711745651487	25.76159334891778	23.965034807416135
41	24.374927551164976	25.237551680168984	26.155880913434913	24.231639855231126
42	24.805043314315984	25.644119822991325	25.633413003874576	23.91742385881811
43	24.436641034558264	25.03526024962325	26.13839084457153	24.38970787124696
44	24.117272486497757	25.54602677022479	25.616546758535858	24.7201539847416
45	24.492386706219033	25.631060145624616	25.41878147049471	24.457771677661633
46	24.42253230872766	25.25546716641148	25.491735051528135	24.830265473332723
47	24.49965787884886	25.388609378144494	25.715033205876438	24.396699537130207
48	24.68061058722571	25.674642211406407	25.162170125276322	24.48257707609156
49	24.61598899387144	25.525733426607427	25.254766276745528	24.6035113027756
50	24.584684712968173	25.95172143426962	24.985007031601555	24.478586821160654
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	245.0
1	158.5
2	72.0
3	195.0
4	318.0
5	306.5
6	295.0
7	249.5
8	204.0
9	283.0
10	362.0
11	667.5
12	973.0
13	1683.5
14	2394.0
15	3210.5
16	4027.0
17	4029.0
18	4031.0
19	3840.5
20	3650.0
21	3981.0
22	4312.0
23	5024.5
24	5737.0
25	6999.0
26	8261.0
27	10673.5
28	13086.0
29	15660.0
30	18234.0
31	21417.5
32	24601.0
33	28818.5
34	33036.0
35	37646.5
36	42257.0
37	46245.5
38	50234.0
39	53987.0
40	57740.0
41	61311.0
42	64882.0
43	67370.5
44	69859.0
45	74821.5
46	79784.0
47	83416.0
48	87048.0
49	90007.0
50	92966.0
51	92017.5
52	91069.0
53	90181.0
54	89293.0
55	87466.5
56	85640.0
57	82198.0
58	78756.0
59	72345.0
60	65934.0
61	57862.0
62	49790.0
63	42995.5
64	36201.0
65	30693.5
66	25186.0
67	21496.5
68	17807.0
69	15308.0
70	12809.0
71	10214.5
72	7620.0
73	6565.0
74	5510.0
75	4182.0
76	2854.0
77	2468.0
78	2082.0
79	1747.0
80	1412.0
81	1081.5
82	751.0
83	628.0
84	505.0
85	385.5
86	266.0
87	176.5
88	87.0
89	58.0
90	29.0
91	23.5
92	18.0
93	14.0
94	10.0
95	11.5
96	13.0
97	11.5
98	10.0
99	7.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032843233931568544
2	0.004990883587640319
3	2.4149436714388634E-4
4	0.0
5	0.0
6	3.2199248952518184E-4
7	0.0
8	0.0
9	0.0
10	8.049812238129546E-5
11	0.0
12	8.049812238129546E-5
13	0.0
14	0.0
15	0.0
16	6.439849790503637E-4
17	0.0
18	0.0
19	0.0
20	8.049812238129546E-5
21	8.049812238129546E-5
22	0.0
23	8.049812238129546E-5
24	0.001046475590956841
25	1.6099624476259092E-4
26	4.024906119064773E-4
27	4.024906119064773E-4
28	0.0012879699581007274
29	3.2199248952518184E-4
30	8.049812238129546E-4
31	3.2199248952518184E-4
32	7.244831014316591E-4
33	3.2199248952518184E-4
34	0.0014489662028633183
35	4.024906119064773E-4
36	2.4149436714388634E-4
37	0.002817434283345341
38	0.0
39	8.8547934619425E-4
40	8.049812238129546E-5
41	7.244831014316591E-4
42	0.0053128760771655
43	0.005876362933834568
44	0.0051518798324029095
45	0.0024149436714388634
46	0.0024954417938201595
47	0.0012074718357194317
48	0.004105404241446068
49	0.0038639098743021814
50	0.0012879699581007274
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1242265.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.0143233631604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.09503754075628	40.943690690502706
2	15.380460690231251	17.230521971539808
3	5.216669883310094	8.766247011677756
4	2.270881509757238	5.088075648278552
5	1.162463382061885	3.2557299890323756
6	0.6954727656323275	2.3373861830640394
7	0.43395273168776044	1.7015298045959495
8	0.3087502116234336	1.383554735385537
9	0.2209690928388694	1.1139690797586577
>10	1.1125714573430543	10.961902801204301
>50	0.06242770028742362	2.4110189312447328
>100	0.03831866065412795	4.001762227888844
>500	0.0018797756865084163	0.6852233171425286
>1k	1.4459812973141662E-4	0.11938760868417105
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1474	0.11865423239002951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.049812238129546E-5
2	0.0	0.0	0.0	0.0	8.049812238129546E-5
3	0.0	0.0	0.0	0.0	8.049812238129546E-5
4	0.0	0.0	0.0	0.0	8.049812238129546E-5
5	0.0	0.0	0.0	0.0	8.049812238129546E-5
6	0.0	0.0	0.0	0.0	1.6099624476259092E-4
7	0.0	0.0	0.0	0.0	1.6099624476259092E-4
8	0.0	0.0	0.0	0.0	1.6099624476259092E-4
9	0.0	0.0	0.0	0.0	1.6099624476259092E-4
10	0.0	0.0	0.0	0.0	1.6099624476259092E-4
11	0.0	0.0	0.0	0.0	1.6099624476259092E-4
12	0.0	0.0	0.0	0.0	3.2199248952518184E-4
13	0.0	0.0	0.0	0.0	4.024906119064773E-4
14	0.0	0.0	0.0	8.049812238129546E-5	4.024906119064773E-4
15	0.0	0.0	0.0	1.6099624476259092E-4	6.439849790503637E-4
16	0.0	0.0	0.0	1.6099624476259092E-4	6.439849790503637E-4
17	0.0	0.0	0.0	1.6099624476259092E-4	7.244831014316591E-4
18	0.0	0.0	0.0	2.4149436714388637E-4	7.244831014316591E-4
19	0.0	0.0	0.0	2.4149436714388637E-4	7.244831014316591E-4
20	0.0	0.0	0.0	2.4149436714388637E-4	0.0011269737133381365
21	0.0	0.0	0.0	4.8298873428777273E-4	0.001207471835719432
22	0.0	0.0	0.0	6.439849790503637E-4	0.001207471835719432
23	0.0	0.0	0.0	0.0013684680804820228	0.001207471835719432
24	0.0	0.0	0.0	0.002253947426676273	0.001207471835719432
25	0.0	0.0	0.0	0.0031394267728705226	0.001207471835719432
26	0.0	0.0	0.0	0.0039444079966834775	0.0012879699581007274
27	0.0	0.0	0.0	0.004668891098115136	0.0015294643252446137
28	0.0	0.0	0.0	0.005715366689071978	0.0015294643252446137
29	0.0	0.0	0.0	0.008049812238129546	0.0015294643252446137
30	0.0	0.0	0.0	0.012316212724338204	0.0015294643252446137
31	0.0	0.0	0.0	0.01915855312674832	0.0016099624476259092
32	0.0	0.0	0.0	0.026403384141064908	0.0016904605700072046
33	0.0	0.0	0.0	0.034131203889669275	0.0016904605700072046
34	0.0	0.0	0.0	0.04234201237256141	0.0017709586923885
35	0.0	0.0	0.0	0.05328975701641759	0.0017709586923885
36	0.0	0.0	0.0	0.06681344157647523	0.0017709586923885
37	0.0	0.0	0.0	0.08573050033607967	0.0017709586923885
38	0.0	0.0	0.0	0.11269737133381363	0.0018514568147697955
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2055	0.0	22.599083	1
TTAGGAC	340	0.0	21.998491	3
GGTATCA	1050	0.0	21.590769	1
TAGGACG	390	0.0	21.434427	4
GTCCTAC	525	0.0	19.284958	1
GTGTACG	75	0.0012869678	17.608006	1
GACGTGA	275	0.0	17.598793	7
TCCTACA	655	0.0	17.465857	2
ATACCGT	290	0.0	17.447079	6
GTAGGAC	515	0.0	17.086206	3
ACCGTCG	285	0.0	16.98129	8
AACGCAG	2805	0.0	16.783161	6
AATCACG	250	0.0	16.720198	34
AGGACGT	500	0.0	16.718853	5
GGTCTAA	120	6.3791776E-6	16.507505	1
TACCGTC	295	0.0	16.405653	7
GTATAGC	230	1.8189894E-12	16.268267	1
TATGGCG	545	0.0	16.145681	16
CGCAATA	150	1.8382889E-7	16.132875	36
ATCAACG	2940	0.0	16.087332	3
>>END_MODULE