FastQCFastQC Report
Thu 2 Feb 2017
SRR4062179_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062179_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274359
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCG14470.5274111656625079No Hit
TTGTAGAACAGTGTATATCAATGAG12950.47200930168137367No Hit
GTGTATATCAATGAGTTACAATGAA12150.4428504259018294No Hit
GTCCTACAGTGGACATTTCTAAATT11140.40603734523015467No Hit
CTGTAGGACGTGGAATATGGCAAGA10920.39801865439078No Hit
GTCCTAAAGTGTGTATTTCTCATTT10530.38380370244825207No Hit
GTGTATATCAATGAGTTACAATGAG9890.36047660182461666No Hit
CTTTAGGACGTGAAATATGGCGAGG9420.3433457623041344No Hit
GTCCTACAGTGTGCATTTCTCATTT8670.3160093162608115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8540.3112709989466356No Hit
CCATAGGGTCTTCTCGTCTTATTAT8170.2977850188985964No Hit
GTTCTACAGTGTGGTTTTTATCATT7730.28174763721984697No Hit
GATATACACTGTTCTACAATGCCGG7600.277009319905671No Hit
GTATCAACGCAGAGTACTTTTTTTT6830.24894390196785965No Hit
CTGTAGGACCTGGAATATGGCGAGA6160.22452334350249126No Hit
GTTCTACAAATCCCGTTTCCAACGA6120.22306539971351405No Hit
GTATTAGAGGCACTGCCTGCCCAGT5140.18734577688357226No Hit
CTGTAGAACATATTAGATGAGTGAG4870.17750465630797604No Hit
TATCAACGCAGAGTACTTTTTTTTT4650.16948596546860137No Hit
ATTTAGAAATGTCCACTGTAGGACG4350.15855138705127225No Hit
GTAGAACAGTGTATATCAATGAGTT4180.15235512594811906No Hit
GTTATATAATTTAAGCTCCATAGGG4170.15199064000087475No Hit
GTCAGGATACCGCGGCCGTTAAACT4110.14980372431740893No Hit
CCATTGGGATGTCCTGATCCAACAT4090.14907475242292034No Hit
ATGTTCTACAGTGTGGTTTTTATCA4050.1476168086339431No Hit
ATCTATAACTTTATAGATGCAACAC3950.14397194916150008No Hit
CTCTAATACTTGTAATGCTAGAGGT3880.14142054753078995No Hit
CTGTTAGTATGAGTAACAAGAATTC3860.14069157563630133No Hit
CTTCTACACCATTGGGATGTCCTGA3860.14069157563630133No Hit
CTGAAGGACCTGGAATATGGCGAGA3730.1359532583221254No Hit
TTGATATACACTGTTCTACAAATCC3720.1355887723748811No Hit
GTAGAACATATTAGATGAGTGAGTT3700.13485980048039248No Hit
GTCCTTCAGTGTGCATTTCTCATTT3690.13449531453314817No Hit
CTATAGAACTAGTACCGCAAGGGAA3530.12866353937723932No Hit
GAATATGGCAAGAAAACTGAAAATC3480.1268411096410178No Hit
CCTATAACTTCTCTGTTAACCCAAC3450.12574765179928488No Hit
ATAAATAATCCACCTATAACTTCTC3440.12538316585204057No Hit
GACTATAGGCAATAATCACACTATA3430.12501867990479626No Hit
ATCCTGACCGTGCAAAGGTAGCATA3410.12428970801030767No Hit
TTTCTAAATTTTCCACCTTTTTCAG3410.12428970801030767No Hit
GTTATAGATTAACCCAATTTTAAGT3400.12392522206306335No Hit
GATTAAACCTTGTACCTTTTGCATA3380.12319625016857474No Hit
CACTATAAATAATCCACCTATAACT3370.12283176422133045No Hit
ACCTATAACTTCTCTGTTAACCCAA3330.12137382043235323No Hit
CATCTAATATGTTCTACAGTGTGGT3260.11882241880164311No Hit
CTGTTAACCCAACACCGGAATGCCT3250.1184579328543988No Hit
GTTCTACAATGCCGGTTTCCAACGT3210.11699998906542158No Hit
GTTCATGCTAGTCCCTAATTAAGGA3170.11554204527644436No Hit
GTACATGGGAGAAATCGTAAATAGA3140.11444858743471145No Hit
GCCTAAAGGAAAGATCCAAAAAGAT3060.11153269985675703No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3060.11153269985675703No Hit
GTATCAACGCAGAGTACATGGGAGA3050.11116821390951273No Hit
GGTCAGGATACCGCGGCCGTTAAAC3010.1097102701205355No Hit
GTATATCAATGAGTTACAATGAAAA3000.1093457841732912No Hit
GTATCCTGACCGTGCAAAGGTAGCA2960.10788784038431398No Hit
CTGTAGGACATGGAATATGGCAAGA2920.10642989659533676No Hit
GATTAAAGATAAGAGACAGTTGGAC2920.10642989659533676No Hit
GGTATCAACGCAGAGTACTTTTTTT2900.10570092470084816No Hit
CCTCTAGCATTACAAGTATTAGAGG2870.10460746685911525No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2770.1009626073866722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG250.006014324319.0029225
CGTTCAA402.7474808E-416.62755814
GAAAGCG350.00216184116.288229
AGACAGC350.00216184116.288226
TGGCCAG350.002164481216.28524618
CCACCTT951.8189894E-1116.00246213
TAGAAAT1700.015.0850454
TAGGGTC1200.015.03848654
TTTAGAA1400.014.9226912
AAGCGTT456.720019E-414.7827511
AGCGTTC456.729338E-414.7800512
GTTCAAG456.729338E-414.7800515
TTCAAGC456.7386683E-414.7773516
GTATTAT456.7761E-414.7665641
TGTCCAC1650.014.39615310
TAGCCAA400.00525267514.25479511
ACTTATC400.005259047714.2521928
AAAGCGT400.005259047714.25219210
ATTTAGA1400.014.2391881
ATGTCCA1700.013.9727369
Produced by FastQC (version 0.11.5)