##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062179_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	274359
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210348485014162	32.0	32.0	32.0	32.0	32.0
2	30.96763729274418	32.0	32.0	32.0	32.0	32.0
3	30.986025608782654	32.0	32.0	32.0	32.0	32.0
4	30.95510991073739	32.0	32.0	32.0	32.0	32.0
5	31.01243990537945	32.0	32.0	32.0	32.0	32.0
6	34.57237415211456	36.0	36.0	36.0	32.0	36.0
7	34.502086682047974	36.0	36.0	36.0	32.0	36.0
8	34.40799098990738	36.0	36.0	36.0	32.0	36.0
9	34.50822462539957	36.0	36.0	36.0	32.0	36.0
10	34.32271221283064	36.0	36.0	36.0	32.0	36.0
11	34.497661822648425	36.0	36.0	36.0	32.0	36.0
12	34.384970057479435	36.0	36.0	36.0	32.0	36.0
13	34.432251174555965	36.0	36.0	36.0	32.0	36.0
14	34.3810992167197	36.0	36.0	36.0	32.0	36.0
15	34.32066380180712	36.0	36.0	36.0	32.0	36.0
16	34.43178098768402	36.0	36.0	36.0	32.0	36.0
17	34.34712548157706	36.0	36.0	36.0	32.0	36.0
18	34.38568444993603	36.0	36.0	36.0	32.0	36.0
19	34.32033211959513	36.0	36.0	36.0	32.0	36.0
20	34.195820075157	36.0	36.0	36.0	32.0	36.0
21	34.183281758571795	36.0	36.0	36.0	32.0	36.0
22	34.14501437897062	36.0	36.0	36.0	32.0	36.0
23	34.109229148670174	36.0	36.0	36.0	32.0	36.0
24	34.051319621371995	36.0	36.0	36.0	32.0	36.0
25	33.549247518761916	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	8.0
5	18.0
6	57.0
7	12.0
8	43.0
9	43.0
10	31.0
11	7.0
12	11.0
13	8.0
14	130.0
15	133.0
16	215.0
17	239.0
18	338.0
19	441.0
20	592.0
21	668.0
22	856.0
23	1133.0
24	1367.0
25	1713.0
26	2195.0
27	3007.0
28	3827.0
29	5337.0
30	7522.0
31	11057.0
32	17227.0
33	27329.0
34	67026.0
35	121769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43615799852018	21.73044609760276	12.684203428379812	24.14919247549725
2	12.18335167919288	22.45715452073568	45.89733122416696	19.462162575904475
3	18.91433820045342	27.23376026942899	31.77199133978233	22.079910190335255
4	9.561771867663998	19.244200457839405	42.80506831138912	28.388959363107475
5	9.521673977007975	41.841768482766916	38.076851558141826	10.559705982083283
6	26.525081879919178	43.41422850515351	19.287918244086043	10.772771370841266
7	23.723773034117862	34.49990877577084	22.713008575077538	19.063309615033752
8	21.03792036431574	43.08624766464269	20.901083021952356	14.97474894908921
9	24.615008466164536	16.111986921235477	21.78906113154668	37.4839434810533
10	13.790271659190534	30.580364475168164	36.24567947646386	19.38368438917743
11	32.753176686171514	23.616439556267775	27.772322585590747	15.858061171969965
12	21.574202496532592	27.070589094094462	35.036499014526605	16.31870939484634
13	27.951369245537133	23.228813466528944	28.340809466280753	20.47900782165317
14	18.359109852493596	26.696396639685865	28.52033778309771	26.42415572472283
15	21.376976899520763	35.49166535877099	24.319194676925434	18.812163064782812
16	19.282828688312208	29.694439477266343	32.115056875202995	18.907674959218454
17	17.235502353928688	30.833546220940843	31.13499507317251	20.795956351957958
18	17.014731479825866	31.070030178186315	34.70491426402811	17.210324077959708
19	22.10489829426252	27.87521663778163	28.106540180607496	21.913344887348355
20	20.41146167766038	32.58493374584087	29.647641702177342	17.355962874321406
21	20.961845763194187	28.807579770738933	27.68535341374253	22.54522105232435
22	19.649118966039417	32.252155604937734	28.944766158370825	19.153959270652027
23	21.11712645103862	31.000605587456313	28.90220857015694	18.980059391348124
24	21.791256061031717	30.633703896937835	29.557403306249476	18.017636735780975
25	20.894531392506657	30.816460545036666	31.036445222720804	17.252562839735873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	910.0
1	910.0
2	555.5
3	201.0
4	201.0
5	201.0
6	574.5
7	948.0
8	948.0
9	948.0
10	976.0
11	1004.0
12	1004.0
13	1004.0
14	1359.0
15	1714.0
16	1714.0
17	1714.0
18	3114.0
19	4514.0
20	4514.0
21	4514.0
22	7421.5
23	10329.0
24	10329.0
25	10329.0
26	16711.5
27	23094.0
28	23094.0
29	23094.0
30	30682.0
31	38270.0
32	38270.0
33	38270.0
34	38144.5
35	38019.0
36	38019.0
37	38019.0
38	36651.5
39	35284.0
40	35284.0
41	35284.0
42	33430.0
43	31576.0
44	31576.0
45	31576.0
46	31823.0
47	32070.0
48	32070.0
49	32070.0
50	27801.0
51	23532.0
52	23532.0
53	23532.0
54	19057.0
55	14582.0
56	14582.0
57	14582.0
58	11474.5
59	8367.0
60	8367.0
61	8367.0
62	6570.0
63	4773.0
64	4773.0
65	4773.0
66	3698.0
67	2623.0
68	2623.0
69	2623.0
70	2013.5
71	1404.0
72	1404.0
73	1404.0
74	1053.0
75	702.0
76	702.0
77	702.0
78	466.0
79	230.0
80	230.0
81	230.0
82	148.5
83	67.0
84	67.0
85	67.0
86	47.0
87	27.0
88	27.0
89	27.0
90	20.0
91	13.0
92	13.0
93	13.0
94	11.0
95	9.0
96	9.0
97	9.0
98	53.0
99	97.0
100	97.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.28971894488608E-4
2	3.64485947244304E-4
3	3.64485947244304E-4
4	0.009841120575596208
5	0.032803735251987355
6	0.0645140126622418
7	0.11262615769848992
8	0.11335512959297854
9	0.1191869047488874
10	0.1370467161638583
11	0.14943923837016465
12	0.13814017400559123
13	0.1370467161638583
14	0.12283176422133045
15	0.13923363184732412
16	0.12319625016857474
17	0.12720559558826208
18	0.11663550311817729
19	0.10351400901738234
20	0.09585980412525195
21	0.09513083223076335
22	0.11153269985675703
23	0.08929905707485447
24	0.09914017765045068
25	0.09075700086383169
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	274359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.250595023308875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.18093525968474	23.00307261653527
2	14.889395567053251	9.901625242838763
3	5.503913358033894	5.490251823340951
4	2.7020805472124176	3.593831439828837
5	1.553285247626773	2.5823829362258937
6	0.9734067042290575	1.9419811269176517
7	0.6292065858417556	1.4645045360276134
8	0.5316466796746542	1.4142054753078994
9	0.44833709688027534	1.3416727718062829
>10	2.967355797689255	19.731446754070397
>50	0.3573542630390459	8.184167459423602
>100	0.24444785477824305	15.649204144934192
>500	0.012057965930765352	3.07152307742775
>1k	0.00657707232587201	2.6301305953148977
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATATACACTGTTCTACAAATCCCG	1447	0.5274111656625079	No Hit
TTGTAGAACAGTGTATATCAATGAG	1295	0.47200930168137367	No Hit
GTGTATATCAATGAGTTACAATGAA	1215	0.4428504259018294	No Hit
GTCCTACAGTGGACATTTCTAAATT	1114	0.40603734523015467	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1092	0.39801865439078	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1053	0.38380370244825207	No Hit
GTGTATATCAATGAGTTACAATGAG	989	0.36047660182461666	No Hit
CTTTAGGACGTGAAATATGGCGAGG	942	0.3433457623041344	No Hit
GTCCTACAGTGTGCATTTCTCATTT	867	0.3160093162608115	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	854	0.3112709989466356	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	817	0.2977850188985964	No Hit
GTTCTACAGTGTGGTTTTTATCATT	773	0.28174763721984697	No Hit
GATATACACTGTTCTACAATGCCGG	760	0.277009319905671	No Hit
GTATCAACGCAGAGTACTTTTTTTT	683	0.24894390196785965	No Hit
CTGTAGGACCTGGAATATGGCGAGA	616	0.22452334350249126	No Hit
GTTCTACAAATCCCGTTTCCAACGA	612	0.22306539971351405	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	514	0.18734577688357226	No Hit
CTGTAGAACATATTAGATGAGTGAG	487	0.17750465630797604	No Hit
TATCAACGCAGAGTACTTTTTTTTT	465	0.16948596546860137	No Hit
ATTTAGAAATGTCCACTGTAGGACG	435	0.15855138705127225	No Hit
GTAGAACAGTGTATATCAATGAGTT	418	0.15235512594811906	No Hit
GTTATATAATTTAAGCTCCATAGGG	417	0.15199064000087475	No Hit
GTCAGGATACCGCGGCCGTTAAACT	411	0.14980372431740893	No Hit
CCATTGGGATGTCCTGATCCAACAT	409	0.14907475242292034	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	405	0.1476168086339431	No Hit
ATCTATAACTTTATAGATGCAACAC	395	0.14397194916150008	No Hit
CTCTAATACTTGTAATGCTAGAGGT	388	0.14142054753078995	No Hit
CTGTTAGTATGAGTAACAAGAATTC	386	0.14069157563630133	No Hit
CTTCTACACCATTGGGATGTCCTGA	386	0.14069157563630133	No Hit
CTGAAGGACCTGGAATATGGCGAGA	373	0.1359532583221254	No Hit
TTGATATACACTGTTCTACAAATCC	372	0.1355887723748811	No Hit
GTAGAACATATTAGATGAGTGAGTT	370	0.13485980048039248	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	369	0.13449531453314817	No Hit
CTATAGAACTAGTACCGCAAGGGAA	353	0.12866353937723932	No Hit
GAATATGGCAAGAAAACTGAAAATC	348	0.1268411096410178	No Hit
CCTATAACTTCTCTGTTAACCCAAC	345	0.12574765179928488	No Hit
ATAAATAATCCACCTATAACTTCTC	344	0.12538316585204057	No Hit
GACTATAGGCAATAATCACACTATA	343	0.12501867990479626	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	341	0.12428970801030767	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	341	0.12428970801030767	No Hit
GTTATAGATTAACCCAATTTTAAGT	340	0.12392522206306335	No Hit
GATTAAACCTTGTACCTTTTGCATA	338	0.12319625016857474	No Hit
CACTATAAATAATCCACCTATAACT	337	0.12283176422133045	No Hit
ACCTATAACTTCTCTGTTAACCCAA	333	0.12137382043235323	No Hit
CATCTAATATGTTCTACAGTGTGGT	326	0.11882241880164311	No Hit
CTGTTAACCCAACACCGGAATGCCT	325	0.1184579328543988	No Hit
GTTCTACAATGCCGGTTTCCAACGT	321	0.11699998906542158	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	317	0.11554204527644436	No Hit
GTACATGGGAGAAATCGTAAATAGA	314	0.11444858743471145	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	306	0.11153269985675703	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	306	0.11153269985675703	No Hit
GTATCAACGCAGAGTACATGGGAGA	305	0.11116821390951273	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	301	0.1097102701205355	No Hit
GTATATCAATGAGTTACAATGAAAA	300	0.1093457841732912	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	296	0.10788784038431398	No Hit
CTGTAGGACATGGAATATGGCAAGA	292	0.10642989659533676	No Hit
GATTAAAGATAAGAGACAGTTGGAC	292	0.10642989659533676	No Hit
GGTATCAACGCAGAGTACTTTTTTT	290	0.10570092470084816	No Hit
CCTCTAGCATTACAAGTATTAGAGG	287	0.10460746685911525	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	277	0.1009626073866722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.64485947244304E-4	0.0
10	0.0	0.0	0.0	7.28971894488608E-4	0.0
11	0.0	0.0	0.0	7.28971894488608E-4	0.0
12	0.0	0.0	0.0	7.28971894488608E-4	0.0
13	0.0	0.0	0.0	7.28971894488608E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACCG	25	0.0060143243	19.002922	5
CGTTCAA	40	2.7474808E-4	16.627558	14
GAAAGCG	35	0.002161841	16.28822	9
AGACAGC	35	0.002161841	16.28822	6
TGGCCAG	35	0.0021644812	16.285246	18
CCACCTT	95	1.8189894E-11	16.002462	13
TAGAAAT	170	0.0	15.085045	4
TAGGGTC	120	0.0	15.0384865	4
TTTAGAA	140	0.0	14.922691	2
AAGCGTT	45	6.720019E-4	14.78275	11
AGCGTTC	45	6.729338E-4	14.78005	12
GTTCAAG	45	6.729338E-4	14.78005	15
TTCAAGC	45	6.7386683E-4	14.77735	16
GTATTAT	45	6.7761E-4	14.766564	1
TGTCCAC	165	0.0	14.396153	10
TAGCCAA	40	0.005252675	14.254795	11
ACTTATC	40	0.0052590477	14.252192	8
AAAGCGT	40	0.0052590477	14.252192	10
ATTTAGA	140	0.0	14.239188	1
ATGTCCA	170	0.0	13.972736	9
>>END_MODULE