FastQCFastQC Report
Thu 2 Feb 2017
SRR4062179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274359
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG8390.30580370973797105No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG7630.27810277774740394No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC6370.23217754839462162No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC6160.22452334350249126No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA5740.2092149337182305No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA5220.1902616644615267No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT5190.18916820661979378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.1734953108882887No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA4470.1629252184182039No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA4220.15381306973709627No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC3990.14542989295047729No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC3930.1432429772670115No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC3920.14287849131976715No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA3860.14069157563630133No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT3790.13814017400559123No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT3750.136682230216614No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA3720.1355887723748811No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA3700.13485980048039248No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC3620.13194391290243807No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG3520.128299053429995No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA3520.128299053429995No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAGTG3450.12574765179928488No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC3420.12465419395755196No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG3370.12283176422133045No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC3340.12173830637959752No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA3310.12064484853786463No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT3240.11809344690715448No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC3220.11736447501266589No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC3200.11663550311817729No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA3200.11663550311817729No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC3170.11554204527644436No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA3040.11080372796226842No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC3000.1093457841732912No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC2970.10825232633155828No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG2940.10715886848982538No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT2910.10606541064809247No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT2900.10570092470084816No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT2820.10278503712289372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT454.783942E-1043.994531
GTATTAT353.2137724E-431.4246651
GGCCAGA353.2137724E-431.42466519
GTGCTAG353.2137724E-431.4246651
ATGCGTA353.2137724E-431.42466513
CCTGGTC353.2137724E-431.4246658
CGTAGAG300.005740860529.32968916
GGAGAGC407.015809E-427.50159526
GCCAGAA407.015809E-427.50159520
ACACCTA804.290996E-927.4965826
CCTAAGC407.0232904E-427.4965823
TTAACAG407.0232904E-427.4965823
CGCGGTA756.942537E-826.41116143
CGGCCGC756.954906E-826.40634539
AACGGCC756.967093E-826.4015337
TACACCT858.187271E-925.8791355
CAGTGAT2150.025.58287837
GGCCGCG801.299104E-724.75594740
TAGGACC3050.024.5215444
CTAGTTC450.00139499224.4458625
Produced by FastQC (version 0.11.5)