##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062179_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 274359 Sequences flagged as poor quality 0 Sequence length 50 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.128058492704813 32.0 32.0 32.0 32.0 32.0 2 31.415674353675293 32.0 32.0 32.0 32.0 32.0 3 31.563826956651685 32.0 32.0 32.0 32.0 32.0 4 31.64476835095623 32.0 32.0 32.0 32.0 32.0 5 31.63555414620989 32.0 32.0 32.0 32.0 32.0 6 35.23560371629872 36.0 36.0 36.0 36.0 36.0 7 35.26569932096268 36.0 36.0 36.0 36.0 36.0 8 35.202034560557514 36.0 36.0 36.0 36.0 36.0 9 35.296534832099546 36.0 36.0 36.0 36.0 36.0 10 35.20698063486162 36.0 36.0 36.0 36.0 36.0 11 35.316384736786475 36.0 36.0 36.0 36.0 36.0 12 35.24579474338367 36.0 36.0 36.0 36.0 36.0 13 35.28743361799686 36.0 36.0 36.0 36.0 36.0 14 35.24907876176834 36.0 36.0 36.0 36.0 36.0 15 35.21642082089525 36.0 36.0 36.0 36.0 36.0 16 35.24386296786327 36.0 36.0 36.0 36.0 36.0 17 35.232155679237785 36.0 36.0 36.0 36.0 36.0 18 35.2507772662825 36.0 36.0 36.0 36.0 36.0 19 35.18442988930562 36.0 36.0 36.0 36.0 36.0 20 35.180012319625014 36.0 36.0 36.0 36.0 36.0 21 35.14571054712985 36.0 36.0 36.0 36.0 36.0 22 35.11904475522946 36.0 36.0 36.0 36.0 36.0 23 35.12625793212543 36.0 36.0 36.0 36.0 36.0 24 35.09602382280151 36.0 36.0 36.0 36.0 36.0 25 35.05988504113224 36.0 36.0 36.0 36.0 36.0 26 35.065297657448816 36.0 36.0 36.0 36.0 36.0 27 35.009779157964566 36.0 36.0 36.0 36.0 36.0 28 34.95736243389136 36.0 36.0 36.0 36.0 36.0 29 34.91873056834294 36.0 36.0 36.0 36.0 36.0 30 34.86577440506781 36.0 36.0 36.0 32.0 36.0 31 34.87088814290765 36.0 36.0 36.0 32.0 36.0 32 34.82022824110017 36.0 36.0 36.0 32.0 36.0 33 34.81278179319796 36.0 36.0 36.0 32.0 36.0 34 34.802820392259775 36.0 36.0 36.0 32.0 36.0 35 34.754478621076764 36.0 36.0 36.0 32.0 36.0 36 34.6997947944117 36.0 36.0 36.0 32.0 36.0 37 34.61224526988362 36.0 36.0 36.0 32.0 36.0 38 34.58575807609738 36.0 36.0 36.0 32.0 36.0 39 34.411107344756324 36.0 36.0 36.0 32.0 36.0 40 34.50461256966238 36.0 36.0 36.0 32.0 36.0 41 34.43693481897805 36.0 36.0 36.0 32.0 36.0 42 34.43792986561403 36.0 36.0 36.0 32.0 36.0 43 34.4749288341188 36.0 36.0 36.0 32.0 36.0 44 34.3218702502925 36.0 36.0 36.0 32.0 36.0 45 34.28824277679974 36.0 36.0 36.0 32.0 36.0 46 34.0958670938442 36.0 36.0 36.0 32.0 36.0 47 34.11186802692822 36.0 36.0 36.0 32.0 36.0 48 34.0029231772969 36.0 36.0 36.0 32.0 36.0 49 33.95327290156328 36.0 36.0 36.0 32.0 36.0 50 33.5963755517406 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 3.0 20 1.0 21 3.0 22 8.0 23 35.0 24 108.0 25 242.0 26 565.0 27 1227.0 28 2225.0 29 3799.0 30 6027.0 31 9211.0 32 14270.0 33 24083.0 34 52691.0 35 159861.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.25200341254621 22.17608155110434 13.212678921693733 24.35923611465572 2 11.92326478242803 22.69469939419565 45.738957374993625 19.6430784483827 3 18.65883262015323 27.177629228963617 32.34678777363882 21.816750377244333 4 9.51271873712909 19.36076454572294 43.09936980379722 28.027146913350755 5 9.440915005521962 41.934837202351666 37.96850112443914 10.655746667687227 6 26.88339001089813 43.239696893486276 19.30973651310874 10.56717658250686 7 23.791091234477456 34.365193049981954 22.806250205023346 19.037465510517244 8 21.161689611057046 42.906556737704975 20.81324104549149 15.118512605746485 9 24.621025736352735 15.620045269154648 22.001100747560677 37.75782824693194 10 13.74950338789688 30.195473813507117 36.221155493349954 19.83386730524605 11 32.64153900546364 23.505698737785163 27.96882916179167 15.883933094959524 12 21.53783910861317 26.725203109794105 35.182006057756446 16.554951723836286 13 27.832146931575053 22.998334299221092 28.76012815325905 20.409390615944805 14 18.37009174111292 26.385502206962407 28.805688896664588 26.43871715526008 15 21.477334441370612 35.10109017746821 24.65638087323543 18.76519450792575 16 19.45785965001804 29.363566448095003 32.19928778926727 18.97928611261969 17 17.130475034535046 30.425828932165523 31.490127898118885 20.953568135180547 18 16.980671310217634 30.41635229753717 35.25526773315255 17.347708659092646 19 22.23838109921672 27.2179881104684 28.78199731009371 21.76163348022117 20 20.303763695609387 31.974646265098883 30.360332120805662 17.361257918486064 21 21.096811112447558 27.92946467948928 28.499520700979375 22.474203507083786 22 19.741287874645995 31.366202676055828 29.75663273302498 19.135876716273202 23 21.192383673885946 30.582304871736927 29.284001195518268 18.941310258858863 24 21.94784130050482 30.150717136556647 29.991069964097612 17.910371598840918 25 21.198867177675965 30.08539905743934 31.373492395000714 17.342241369883986 26 16.615881439578946 31.52085960679113 34.27055161504312 17.59270733858681 27 17.739531052380276 31.21421203605495 32.06893158234284 18.97732532922193 28 17.43033660767983 32.05226804687358 30.257148584862676 20.260246760583914 29 18.92957767013293 28.082913263279135 29.22047390462861 23.76703516195933 30 18.68477932037455 30.26203085760524 30.51826634640268 20.534923475617532 31 20.96801236349852 28.007041945501467 31.09281371648515 19.932131974514864 32 18.576297133276228 31.195713582766853 30.855278744692093 19.37271053926482 33 19.428923417857625 30.77464198367832 31.667268068479622 18.129166529984435 34 19.0456230385957 31.011054939367323 32.833133472082 17.110188549954984 35 18.731730075303073 32.09674950247487 31.10097026512804 18.070550157094015 36 17.43154042695884 31.02795971701311 31.61733349370715 19.923166362320902 37 18.94543850760522 30.13220290795368 29.203101136864817 21.71925744757628 38 19.93154953910752 31.86190356430808 29.412557998826355 18.793988897758044 39 20.537699468569805 28.95018844266896 29.83298949532356 20.679122593437675 40 22.54179773069395 29.695980055183576 30.663697299503934 17.098524914618544 41 20.677878376999228 27.335651489305867 34.15343568210646 17.833034451588446 42 19.933002598974262 32.74962728594913 29.936684175418005 17.380685939658598 43 17.020663913422247 32.685725533365165 32.06205370649151 18.231556846721077 44 17.30230184621553 32.36800379084729 30.507572581967963 19.82212178096922 45 17.569592016008805 31.916646315459506 31.302829607543675 19.21093206098801 46 19.82219727427474 28.99409146743746 28.995549464368374 22.188161793919424 47 21.471372960678824 30.366749770371342 28.97002434792751 19.19185292102232 48 19.1321844108127 30.018516898492404 28.264150118099902 22.58514857259499 49 20.124587200104976 30.056206396302482 30.230438934775794 19.58876746881675 50 18.676760571080745 31.594601194793643 28.371428467288972 21.35720976683664 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 306.0 1 167.5 2 29.0 3 84.5 4 140.0 5 124.0 6 108.0 7 83.0 8 58.0 9 93.0 10 128.0 11 212.5 12 297.0 13 514.0 14 731.0 15 967.0 16 1203.0 17 1490.5 18 1778.0 19 1687.5 20 1597.0 21 1971.5 22 2346.0 23 2532.5 24 2719.0 25 4260.0 26 5801.0 27 9078.0 28 12355.0 29 13995.0 30 15635.0 31 16091.0 32 16547.0 33 19204.0 34 21861.0 35 25278.0 36 28695.0 37 29939.5 38 31184.0 39 28963.0 40 26742.0 41 24268.0 42 21794.0 43 17721.0 44 13648.0 45 12847.0 46 12046.0 47 11376.5 48 10707.0 49 9620.5 50 8534.0 51 8337.0 52 8140.0 53 7446.5 54 6753.0 55 6213.5 56 5674.0 57 5350.0 58 5026.0 59 4369.0 60 3712.0 61 3249.0 62 2786.0 63 2243.5 64 1701.0 65 1495.0 66 1289.0 67 1104.0 68 919.0 69 770.0 70 621.0 71 490.0 72 359.0 73 269.0 74 179.0 75 138.5 76 98.0 77 78.0 78 58.0 79 40.5 80 23.0 81 19.0 82 15.0 83 12.5 84 10.0 85 7.0 86 4.0 87 2.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028065417937811407 2 0.0047383173141759515 3 3.64485947244304E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 7.28971894488608E-4 17 0.0 18 0.0 19 0.0 20 3.64485947244304E-4 21 0.0 22 0.0 23 3.64485947244304E-4 24 0.001457943788977216 25 0.0 26 3.64485947244304E-4 27 0.0 28 0.001457943788977216 29 0.0 30 7.28971894488608E-4 31 0.001093457841732912 32 0.001457943788977216 33 0.0 34 7.28971894488608E-4 35 3.64485947244304E-4 36 0.0 37 0.002915887577954432 38 0.0 39 0.0018224297362215201 40 7.28971894488608E-4 41 0.001093457841732912 42 0.0072897189448860806 43 0.005831775155908864 44 0.005102803261420256 45 0.0036448594724430403 46 0.0036448594724430403 47 0.001093457841732912 48 0.00546728920866456 49 0.0047383173141759515 50 7.28971894488608E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 274359.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.188366803003085 #Duplication Level Percentage of deduplicated Percentage of total 1 68.4722978670695 26.148452267921357 2 15.744282497750886 12.024968701484248 3 5.8532061247452365 6.70573147396066 4 2.7138447934667616 4.145492016773153 5 1.5617413854189548 2.9820176438904626 6 1.0025721841917241 2.297195658984092 7 0.7025925999720543 1.8781604744565896 8 0.4953023429282988 1.5131830040106158 9 0.38468143927322906 1.3221320314745904 >10 2.625002294827908 19.601729582185307 >50 0.24715283553398104 6.507903616537691 >100 0.1903475467217354 13.171844688208742 >500 0.006976088099749465 1.7011888401124844 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 839 0.30580370973797105 No Hit GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG 763 0.27810277774740394 No Hit GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC 637 0.23217754839462162 No Hit GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC 616 0.22452334350249126 No Hit TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA 574 0.2092149337182305 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 522 0.1902616644615267 No Hit GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT 519 0.18916820661979378 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 476 0.1734953108882887 No Hit TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA 447 0.1629252184182039 No Hit CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA 422 0.15381306973709627 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 399 0.14542989295047729 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 393 0.1432429772670115 No Hit GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC 392 0.14287849131976715 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 386 0.14069157563630133 No Hit CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT 379 0.13814017400559123 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 375 0.136682230216614 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 372 0.1355887723748811 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 370 0.13485980048039248 No Hit GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC 362 0.13194391290243807 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 352 0.128299053429995 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 352 0.128299053429995 No Hit GATATACACTGTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAGTG 345 0.12574765179928488 No Hit GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC 342 0.12465419395755196 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 337 0.12283176422133045 No Hit GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC 334 0.12173830637959752 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 331 0.12064484853786463 No Hit ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT 324 0.11809344690715448 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 322 0.11736447501266589 No Hit ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC 320 0.11663550311817729 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 320 0.11663550311817729 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 317 0.11554204527644436 No Hit GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA 304 0.11080372796226842 No Hit GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC 300 0.1093457841732912 No Hit GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC 297 0.10825232633155828 No Hit GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG 294 0.10715886848982538 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 291 0.10606541064809247 No Hit CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT 290 0.10570092470084816 No Hit GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT 282 0.10278503712289372 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 3.64485947244304E-4 0.0 20 0.0 0.0 0.0 7.28971894488608E-4 0.0 21 0.0 0.0 0.0 7.28971894488608E-4 0.0 22 0.0 0.0 0.0 0.001093457841732912 0.0 23 0.0 0.0 0.0 0.001457943788977216 0.0 24 0.0 0.0 0.0 0.001457943788977216 0.0 25 0.0 0.0 0.0 0.002186915683465824 0.0 26 0.0 0.0 0.0 0.002186915683465824 0.0 27 0.0 0.0 0.0 0.002186915683465824 0.0 28 0.0 0.0 0.0 0.002551401630710128 0.0 29 0.0 0.0 0.0 0.003280373525198736 0.0 30 0.0 0.0 0.0 0.005831775155908864 0.0 31 0.0 0.0 0.0 0.00728971894488608 0.0 32 0.0 0.0 0.0 0.0091121486811076 0.0 33 0.0 0.0 0.0 0.013850465995283551 0.0 34 0.0 0.0 0.0 0.01931775520394811 0.0 35 0.0 0.0 0.0 0.02186915683465824 0.0 36 0.0 0.0 0.0 0.028429903885055712 0.0 37 0.0 0.0 0.0 0.03899999635514053 0.0 38 0.0 0.0 0.0 0.05212149045593547 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAT 45 4.783942E-10 43.99453 1 GTATTAT 35 3.2137724E-4 31.424665 1 GGCCAGA 35 3.2137724E-4 31.424665 19 GTGCTAG 35 3.2137724E-4 31.424665 1 ATGCGTA 35 3.2137724E-4 31.424665 13 CCTGGTC 35 3.2137724E-4 31.424665 8 CGTAGAG 30 0.0057408605 29.329689 16 GGAGAGC 40 7.015809E-4 27.501595 26 GCCAGAA 40 7.015809E-4 27.501595 20 ACACCTA 80 4.290996E-9 27.496582 6 CCTAAGC 40 7.0232904E-4 27.496582 3 TTAACAG 40 7.0232904E-4 27.496582 3 CGCGGTA 75 6.942537E-8 26.411161 43 CGGCCGC 75 6.954906E-8 26.406345 39 AACGGCC 75 6.967093E-8 26.40153 37 TACACCT 85 8.187271E-9 25.879135 5 CAGTGAT 215 0.0 25.582878 37 GGCCGCG 80 1.299104E-7 24.755947 40 TAGGACC 305 0.0 24.521544 4 CTAGTTC 45 0.001394992 24.44586 25 >>END_MODULE