FastQCFastQC Report
Thu 2 Feb 2017
SRR4062178_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062178_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155738
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23310.2016893102069846No Hit
TATCAACGCAGAGTACTTTTTTTTT14830.12831627929513437No Hit
GCGCAAGACGGACCAGAGCGAAAGC13510.11689500561545955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1350.014.07667359
GCGTAAC1350.014.076673511
CGCGTAA1450.013.10586910
CGTCGTA2400.013.06491410
CGACCCG604.0902142E-412.6668125
GGTATCA6700.012.6140831
GTCGTAG2650.012.1909311
GTATAAA1251.4169927E-912.1548731
GGGTCGC1800.012.1416586
CCGTCGT2600.012.0599199
CGACCAT2550.011.92377110
CCGACCA2600.011.6944689
CGTCAAT1950.011.69244119
TTAGAGT1501.7644197E-1011.3986484
TATAGGT752.0768886E-411.397662
GTATCAA15100.011.3197291
CGTTATT1104.9765185E-711.224972
CGGTTCT2050.011.122560512
GGTCGCG1803.6379788E-1211.0858617
AGCCCCG2500.011.01869316
Produced by FastQC (version 0.11.5)