##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062178_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1155738
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258854515469768	32.0	32.0	32.0	32.0	32.0
2	30.805472347538974	32.0	32.0	32.0	32.0	32.0
3	30.835638354021413	32.0	32.0	32.0	32.0	32.0
4	30.83306510645146	32.0	32.0	32.0	32.0	32.0
5	30.768027009581758	32.0	32.0	32.0	32.0	32.0
6	34.42210864400063	36.0	36.0	36.0	32.0	36.0
7	34.349107669731374	36.0	36.0	36.0	32.0	36.0
8	34.30682473017241	36.0	36.0	36.0	32.0	36.0
9	34.44665226893984	36.0	36.0	36.0	32.0	36.0
10	34.149746741908636	36.0	36.0	36.0	32.0	36.0
11	34.43497315135437	36.0	36.0	36.0	32.0	36.0
12	34.25117890040823	36.0	36.0	36.0	32.0	36.0
13	34.33710235364762	36.0	36.0	36.0	32.0	36.0
14	34.242188108377505	36.0	36.0	36.0	32.0	36.0
15	34.20496860014986	36.0	36.0	36.0	32.0	36.0
16	34.20793813130658	36.0	36.0	36.0	32.0	36.0
17	34.13479439111632	36.0	36.0	36.0	32.0	36.0
18	34.149675791572136	36.0	36.0	36.0	32.0	36.0
19	34.14952437317108	36.0	36.0	36.0	32.0	36.0
20	34.129101924484615	36.0	36.0	36.0	32.0	36.0
21	34.113451318551434	36.0	36.0	36.0	32.0	36.0
22	34.080345199344485	36.0	36.0	36.0	32.0	36.0
23	34.05903933244386	36.0	36.0	36.0	32.0	36.0
24	34.03142840332325	36.0	36.0	36.0	32.0	36.0
25	33.67987640797482	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	17.0
5	78.0
6	245.0
7	67.0
8	203.0
9	166.0
10	93.0
11	34.0
12	42.0
13	56.0
14	207.0
15	282.0
16	425.0
17	592.0
18	753.0
19	1029.0
20	1565.0
21	2230.0
22	3326.0
23	4976.0
24	7010.0
25	9744.0
26	13298.0
27	16994.0
28	22401.0
29	29896.0
30	38986.0
31	52084.0
32	74188.0
33	106114.0
34	235686.0
35	532951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87620768244376	17.763639478561526	12.162225302537106	25.197927536457605
2	16.722735028536025	19.267269574607262	37.72267273035617	26.28732266650054
3	19.411937248156356	22.653792807470968	28.26420080174643	29.670069142626243
4	13.05297060803619	15.625438075772182	35.07747342989316	36.24411788629846
5	15.264660264089017	36.1870849807161	33.00576093504407	15.54249382015081
6	34.86695030745068	34.753009137804746	16.451252054144565	13.928788500600009
7	30.96573462371664	30.237706898896395	19.59031225070139	19.206246226685572
8	28.33350945971932	33.03712339992066	18.81315646779399	19.816210672566033
9	26.632379655449324	15.141206525100639	18.83438022199064	39.3920335974594
10	16.919022138431043	26.5830121851477	30.302497331466515	26.195468344954737
11	36.75425994825503	21.805840283782825	21.6293424785248	19.810557289437337
12	24.500235229290077	23.908306496400517	28.053453798454854	23.538004475854557
13	29.14553839302964	19.61102317590766	25.023759832065423	26.21967859899728
14	23.491821214512612	19.949427792537872	24.859124610508243	31.699626382441277
15	25.1238095568155	27.199200826205693	22.088266077047837	25.588723539930964
16	25.81375927591148	26.2483821840126	23.26345754348395	24.674400996591974
17	24.033998270804492	26.462668956089175	24.774970674715455	24.72836209839088
18	24.728286056074378	25.559431442849334	25.64821977233806	24.06406272873823
19	25.41046576182463	25.375648599561927	25.21403460756641	23.999851031047033
20	25.55386482069598	24.7409841347099	24.870026561954656	24.835124482639465
21	26.592531968423465	24.56248836232468	24.584832698944705	24.260146970307147
22	25.86909072011364	24.47321010965406	25.19410327922809	24.463595891004207
23	24.517457604074927	24.624665192778817	25.479294993197776	25.378582209948487
24	24.442450575974906	25.192200933442226	25.40265413531881	24.96269435526406
25	24.735597741365574	24.728322998579692	25.302681262341082	25.233397997713652
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	601.0
1	601.0
2	501.0
3	401.0
4	401.0
5	401.0
6	798.5
7	1196.0
8	1196.0
9	1196.0
10	1461.0
11	1726.0
12	1726.0
13	1726.0
14	2395.5
15	3065.0
16	3065.0
17	3065.0
18	5258.5
19	7452.0
20	7452.0
21	7452.0
22	11789.0
23	16126.0
24	16126.0
25	16126.0
26	23727.5
27	31329.0
28	31329.0
29	31329.0
30	39320.5
31	47312.0
32	47312.0
33	47312.0
34	59087.0
35	70862.0
36	70862.0
37	70862.0
38	82730.0
39	94598.0
40	94598.0
41	94598.0
42	108864.5
43	123131.0
44	123131.0
45	123131.0
46	136451.5
47	149772.0
48	149772.0
49	149772.0
50	153989.5
51	158207.0
52	158207.0
53	158207.0
54	147799.5
55	137392.0
56	137392.0
57	137392.0
58	125977.0
59	114562.0
60	114562.0
61	114562.0
62	100236.0
63	85910.0
64	85910.0
65	85910.0
66	70097.0
67	54284.0
68	54284.0
69	54284.0
70	41161.5
71	28039.0
72	28039.0
73	28039.0
74	21420.5
75	14802.0
76	14802.0
77	14802.0
78	11787.5
79	8773.0
80	8773.0
81	8773.0
82	6096.5
83	3420.0
84	3420.0
85	3420.0
86	2476.0
87	1532.0
88	1532.0
89	1532.0
90	1063.5
91	595.0
92	595.0
93	595.0
94	360.0
95	125.0
96	125.0
97	125.0
98	325.5
99	526.0
100	526.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001038297607243164
2	5.19148803621582E-4
3	9.51772806639567E-4
4	0.010036876870017253
5	0.032014176223330894
6	0.06541274925631933
7	0.10806947595389266
8	0.10936734796294661
9	0.11810635282390991
10	0.133767341733161
11	0.13982407777541275
12	0.13368081693255737
13	0.12797018009272
14	0.11672195601425236
15	0.133767341733161
16	0.1214808200474502
17	0.12494181207159409
18	0.11308791438890128
19	0.0982056486850826
20	0.09336025985128117
21	0.09370635905369556
22	0.10365671112310922
23	0.0838425317848855
24	0.09405245825610994
25	0.09154323903860563
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1155738.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.356780938540865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28797382069779	47.062788931359215
2	13.080798279225773	15.528681559224932
3	3.5902770345835298	6.3932186235134605
4	1.4483926906336417	3.4388771060369945
5	0.7272828238969941	2.1584583629208636
6	0.41359528367546994	1.4729810790203124
7	0.27583952110207244	1.1461062219773415
8	0.17710513186655505	0.8409912412235602
9	0.14409778639007678	0.7697862666436
>10	0.724705916981583	7.781946481449306
>50	0.06315594614467382	2.716458225023061
>100	0.05960030332847714	7.238512500509294
>500	0.005711081580858614	2.325828813161687
>1k	0.0014643798925278496	1.125364587936481
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2331	0.2016893102069846	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1483	0.12831627929513437	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1351	0.11689500561545955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.652480060359701E-5
2	0.0	0.0	0.0	0.0	8.652480060359701E-5
3	0.0	0.0	0.0	0.0	8.652480060359701E-5
4	0.0	0.0	0.0	0.0	8.652480060359701E-5
5	0.0	0.0	0.0	0.0	8.652480060359701E-5
6	0.0	0.0	0.0	0.0	1.7304960120719402E-4
7	0.0	0.0	0.0	0.0	1.7304960120719402E-4
8	0.0	0.0	0.0	0.0	2.59574401810791E-4
9	0.0	0.0	0.0	0.0	2.59574401810791E-4
10	0.0	0.0	0.0	0.0	2.59574401810791E-4
11	0.0	0.0	0.0	0.0	2.59574401810791E-4
12	0.0	0.0	0.0	0.0	5.19148803621582E-4
13	0.0	0.0	0.0	0.0	5.19148803621582E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	135	0.0	14.0766735	9
GCGTAAC	135	0.0	14.0766735	11
CGCGTAA	145	0.0	13.105869	10
CGTCGTA	240	0.0	13.064914	10
CGACCCG	60	4.0902142E-4	12.666812	5
GGTATCA	670	0.0	12.614083	1
GTCGTAG	265	0.0	12.19093	11
GTATAAA	125	1.4169927E-9	12.154873	1
GGGTCGC	180	0.0	12.141658	6
CCGTCGT	260	0.0	12.059919	9
CGACCAT	255	0.0	11.923771	10
CCGACCA	260	0.0	11.694468	9
CGTCAAT	195	0.0	11.692441	19
TTAGAGT	150	1.7644197E-10	11.398648	4
TATAGGT	75	2.0768886E-4	11.39766	2
GTATCAA	1510	0.0	11.319729	1
CGTTATT	110	4.9765185E-7	11.22497	2
CGGTTCT	205	0.0	11.1225605	12
GGTCGCG	180	3.6379788E-12	11.085861	7
AGCCCCG	250	0.0	11.018693	16
>>END_MODULE