FastQCFastQC Report
Thu 2 Feb 2017
SRR4062178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155738
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13050.11291486478769411No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12110.10478153353095597No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12040.10417585992673078No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11970.10357018632250563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17100.024.4486051
GGTATCA8350.023.9801271
TGCGAAG500.00258106221.9989832
CTCGCGC550.004484694519.99907530
CGCAATA2650.019.92533136
GTATATA2350.019.662921
TGTACCG1051.7933417E-618.8562725
TCTAGCG2950.018.64320628
CTAGCGG2900.018.20605729
TAGCGGC3250.017.59918830
CTAACAC1408.375537E-817.2849143
CAATACG3200.017.18893638
GTATAGA1551.4357283E-817.0351541
CGCCGGT3000.016.8658877
TATCAAC25400.016.4559332
CGGTCCA3350.016.4171510
TCAACGC25250.016.379444
ATCAACG25400.016.3693223
AACGCAG25450.016.2507236
GCACCGT953.3473334E-416.2097766
Produced by FastQC (version 0.11.5)