##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1155738
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294244889412653	32.0	32.0	32.0	32.0	32.0
2	31.475565396309545	32.0	32.0	32.0	32.0	32.0
3	31.59218179206706	32.0	32.0	32.0	32.0	32.0
4	31.666426992968994	32.0	32.0	32.0	32.0	32.0
5	31.644907409810873	32.0	32.0	32.0	32.0	32.0
6	35.29345059174311	36.0	36.0	36.0	36.0	36.0
7	35.292841457146864	36.0	36.0	36.0	36.0	36.0
8	35.2482223479716	36.0	36.0	36.0	36.0	36.0
9	35.326717647079185	36.0	36.0	36.0	36.0	36.0
10	35.216703958855724	36.0	36.0	36.0	36.0	36.0
11	35.329585078971185	36.0	36.0	36.0	36.0	36.0
12	35.25079386504554	36.0	36.0	36.0	36.0	36.0
13	35.283199133367596	36.0	36.0	36.0	36.0	36.0
14	35.25413372234884	36.0	36.0	36.0	36.0	36.0
15	35.237834180411134	36.0	36.0	36.0	36.0	36.0
16	35.24455110068199	36.0	36.0	36.0	36.0	36.0
17	35.219773858781146	36.0	36.0	36.0	36.0	36.0
18	35.22487968726476	36.0	36.0	36.0	36.0	36.0
19	35.210837577374804	36.0	36.0	36.0	36.0	36.0
20	35.20950682594152	36.0	36.0	36.0	36.0	36.0
21	35.20307889850468	36.0	36.0	36.0	36.0	36.0
22	35.19288541174557	36.0	36.0	36.0	36.0	36.0
23	35.163307773907235	36.0	36.0	36.0	36.0	36.0
24	35.147303281539585	36.0	36.0	36.0	36.0	36.0
25	35.13074416520007	36.0	36.0	36.0	36.0	36.0
26	35.091763877280144	36.0	36.0	36.0	36.0	36.0
27	35.07949725629857	36.0	36.0	36.0	36.0	36.0
28	35.06545514640862	36.0	36.0	36.0	36.0	36.0
29	35.04442010213388	36.0	36.0	36.0	36.0	36.0
30	35.02625508549516	36.0	36.0	36.0	36.0	36.0
31	35.02805220560369	36.0	36.0	36.0	36.0	36.0
32	35.00408397058849	36.0	36.0	36.0	36.0	36.0
33	34.98251766403804	36.0	36.0	36.0	36.0	36.0
34	34.96786815004785	36.0	36.0	36.0	36.0	36.0
35	34.96196715864668	36.0	36.0	36.0	36.0	36.0
36	34.939970823837236	36.0	36.0	36.0	36.0	36.0
37	34.92791272762512	36.0	36.0	36.0	36.0	36.0
38	34.91834741091839	36.0	36.0	36.0	36.0	36.0
39	34.899980791494265	36.0	36.0	36.0	36.0	36.0
40	34.88474204361196	36.0	36.0	36.0	36.0	36.0
41	34.859995085391326	36.0	36.0	36.0	32.0	36.0
42	34.818128330123265	36.0	36.0	36.0	32.0	36.0
43	34.82090837196666	36.0	36.0	36.0	32.0	36.0
44	34.776480482600725	36.0	36.0	36.0	32.0	36.0
45	34.76052357887341	36.0	36.0	36.0	32.0	36.0
46	34.74453379572187	36.0	36.0	36.0	32.0	36.0
47	34.70828250001298	36.0	36.0	36.0	32.0	36.0
48	34.681797258548215	36.0	36.0	36.0	32.0	36.0
49	34.66878738952946	36.0	36.0	36.0	32.0	36.0
50	34.26599194627156	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	13.0
21	26.0
22	79.0
23	229.0
24	517.0
25	1211.0
26	2625.0
27	4936.0
28	8503.0
29	13908.0
30	20528.0
31	30191.0
32	46008.0
33	78404.0
34	179798.0
35	768758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60586639201312	18.18858485626128	12.651863648362681	25.553685103362916
2	16.092855109445157	19.819882961997692	37.350749723326174	26.736512205230973
3	18.786107912094188	22.998571474306008	28.554852877427994	29.660467736171807
4	12.80618963813598	16.047149094344913	35.277026454092535	35.86963481342657
5	14.878372087791526	36.44069849741031	32.942933433009905	15.737995981788261
6	35.137868855863275	34.75490942568199	16.27093038548596	13.836291332968775
7	30.934346711798007	30.289909996902413	19.64346590663282	19.132277384666768
8	28.339294892094923	33.185116349899374	18.670581048645975	19.805007709359735
9	26.867508033827736	14.817372103365988	18.789466124675318	39.52565373813096
10	16.911200385554846	26.69153968420151	30.33631353846074	26.060946391782906
11	37.191301142646516	21.58231363855822	21.55350087995722	19.67288433883804
12	24.504083105340484	23.859905964846703	28.0670013445954	23.569009585217408
13	29.335281871842927	19.480972331099263	25.063119842040326	26.120625955017484
14	23.556809588332303	19.690275823759364	24.966212065364296	31.786702522544036
15	25.15440350667712	27.176920720786196	22.038212812938575	25.63046295959811
16	25.824825716063195	26.255203425716584	23.378837080319002	24.54113377790122
17	24.080976830388895	26.419309566701106	24.70430149393721	24.795412108972794
18	24.896126976875383	25.323819066258963	25.770287037373524	24.009766919492133
19	25.510799160363334	25.149471593042716	25.383434653874843	23.95629459271911
20	25.700399659783013	24.53291547442186	25.018408230966845	24.748276634828287
21	26.745335015375453	24.33146612813631	24.710531279580668	24.212667576907567
22	25.836738084237087	24.381650512486395	25.267318371464814	24.514293031811707
23	24.523291611074484	24.425691635993623	25.625877145166122	25.42513960776577
24	24.60548470999081	25.019338560657324	25.424712863474085	24.95046386587778
25	24.810402366628395	24.51388161839588	25.52354039024449	25.152175624731232
26	24.632052011968153	25.493063282727686	25.691119236779397	24.183765468524765
27	25.15697802696985	24.89973912704902	25.103938186521997	24.83934465945913
28	24.787492515514085	24.8067012539326	25.670056172580995	24.735750057972318
29	24.591817840437333	25.030500097773363	25.58607776529894	24.79160429649037
30	24.60470734412828	25.10309537035595	25.766747481669533	24.525449803846243
31	25.218994649297073	24.896862259201065	24.882066492693834	25.00207659880803
32	25.119880941050244	24.850440846910608	24.878475076358665	25.15120313568048
33	24.65495983075705	24.537631896585292	25.406689249698246	25.400719022959418
34	25.215146150558176	24.83261603528864	25.558999278375673	24.393238535777513
35	25.600223580471663	24.53709151570947	25.48107872386081	24.38160617995805
36	24.502308485674927	25.228512393833885	25.19165276499131	25.077526355499874
37	25.51132715766303	24.986545160670683	25.126977342223622	24.375150339442666
38	24.73735396776778	24.63958094308572	25.542640286985456	25.080424802161044
39	25.449563177654355	24.694890601895075	24.89805171263605	24.95749450781452
40	25.502752353907198	24.915508532210588	25.17387158681293	24.407867527069286
41	24.54413777707788	25.39529402399539	25.543080429376612	24.517487769550115
42	25.607072613352095	25.0839989651016	25.108313885723334	24.20061453582297
43	25.23336748326538	24.378032825468466	25.265469573102777	25.123130118163377
44	24.80288729445073	24.93371890586008	25.102969323794394	25.160424475894793
45	24.963831819692103	25.21709560859453	25.083065677262155	24.73600689445121
46	24.834819867994337	25.011767678340895	25.093362683307546	25.060049770357217
47	24.87743634736953	24.92770777848729	25.391398999416815	24.80345687472636
48	25.623176417246118	25.308300135330175	24.427183021081742	24.64134042634197
49	24.953188705758567	25.574108941389838	24.620491059994603	24.852211292856992
50	25.08522796100107	25.45694300715396	24.721127189536602	24.73670184230837
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	290.0
1	164.5
2	39.0
3	96.5
4	154.0
5	170.0
6	186.0
7	151.5
8	117.0
9	169.5
10	222.0
11	382.5
12	543.0
13	1023.5
14	1504.0
15	1999.0
16	2494.0
17	2576.5
18	2659.0
19	2545.0
20	2431.0
21	2844.0
22	3257.0
23	3858.0
24	4459.0
25	5665.5
26	6872.0
27	8613.0
28	10354.0
29	12336.5
30	14319.0
31	16269.5
32	18220.0
33	20826.0
34	23432.0
35	27083.0
36	30734.0
37	36187.0
38	41640.0
39	46180.0
40	50720.0
41	55201.0
42	59682.0
43	62764.0
44	65846.0
45	71668.0
46	77490.0
47	81663.5
48	85837.0
49	88881.5
50	91926.0
51	89229.5
52	86533.0
53	85751.0
54	84969.0
55	85043.5
56	85118.0
57	81679.0
58	78240.0
59	71404.0
60	64568.0
61	56724.0
62	48880.0
63	42121.5
64	35363.0
65	29768.5
66	24174.0
67	20809.0
68	17444.0
69	15329.5
70	13215.0
71	10328.0
72	7441.0
73	6632.0
74	5823.0
75	4316.5
76	2810.0
77	2478.5
78	2147.0
79	1860.0
80	1573.0
81	1206.5
82	840.0
83	721.5
84	603.0
85	481.5
86	360.0
87	230.5
88	101.0
89	70.0
90	39.0
91	31.5
92	24.0
93	19.0
94	14.0
95	14.5
96	15.0
97	13.5
98	12.0
99	8.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029678006607033774
2	0.0038936160271618656
3	8.652480060359701E-5
4	0.0
5	0.0
6	1.7304960120719402E-4
7	0.0
8	0.0
9	0.0
10	8.652480060359701E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.787232054323731E-4
17	0.0
18	0.0
19	0.0
20	4.3262400301798506E-4
21	0.0
22	0.0
23	0.0
24	0.0013843968096575521
25	8.652480060359701E-5
26	3.4609920241438803E-4
27	2.59574401810791E-4
28	0.001211347208450358
29	3.4609920241438803E-4
30	0.001038297607243164
31	1.7304960120719402E-4
32	6.921984048287761E-4
33	2.59574401810791E-4
34	0.001038297607243164
35	1.7304960120719402E-4
36	1.7304960120719402E-4
37	0.0017304960120719402
38	0.0
39	4.3262400301798506E-4
40	0.0
41	0.0013843968096575521
42	0.005970211241648194
43	0.00432624003017985
44	0.004672339232594238
45	0.0022496448156935226
46	0.0025957440181079102
47	0.001038297607243164
48	0.003980140827765462
49	0.0029418432205222983
50	0.001211347208450358
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1155738.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.734180575484764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98693365540647	47.972066917312915
2	13.403174358105618	16.280616234997876
3	3.70953916589078	6.758874646591314
4	1.407212703543179	3.4186364178043016
5	0.7115624548444818	2.160808131162999
6	0.41107337556620754	1.497972277284727
7	0.26624611370813417	1.131916768322961
8	0.17508655697970787	0.8506990854356385
9	0.1319527219222924	0.721263639658971
>10	0.6803479879309394	7.432995603655561
>50	0.05810022701564907	2.50523535064927
>100	0.052893616474928684	6.514654947613328
>500	0.004873661678007516	2.050655682187411
>1k	0.0010034009337074297	0.7036042973228043
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1305	0.11291486478769411	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1211	0.10478153353095597	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1204	0.10417585992673078	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1197	0.10357018632250563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.652480060359701E-5
2	0.0	0.0	0.0	0.0	8.652480060359701E-5
3	0.0	0.0	0.0	0.0	8.652480060359701E-5
4	0.0	0.0	0.0	0.0	8.652480060359701E-5
5	0.0	0.0	0.0	0.0	8.652480060359701E-5
6	0.0	0.0	0.0	0.0	8.652480060359701E-5
7	0.0	0.0	0.0	0.0	8.652480060359701E-5
8	0.0	0.0	0.0	0.0	8.652480060359701E-5
9	0.0	0.0	0.0	0.0	8.652480060359701E-5
10	0.0	0.0	0.0	0.0	8.652480060359701E-5
11	0.0	0.0	0.0	0.0	2.59574401810791E-4
12	0.0	0.0	0.0	0.0	5.19148803621582E-4
13	0.0	0.0	0.0	0.0	5.19148803621582E-4
14	0.0	0.0	0.0	0.0	5.19148803621582E-4
15	0.0	0.0	0.0	0.0	5.19148803621582E-4
16	0.0	0.0	0.0	0.0	5.19148803621582E-4
17	0.0	0.0	0.0	0.0	5.19148803621582E-4
18	0.0	0.0	0.0	8.652480060359701E-5	5.19148803621582E-4
19	0.0	0.0	0.0	8.652480060359701E-5	5.19148803621582E-4
20	0.0	0.0	0.0	2.59574401810791E-4	5.19148803621582E-4
21	0.0	0.0	0.0	3.4609920241438803E-4	5.19148803621582E-4
22	0.0	0.0	0.0	3.4609920241438803E-4	5.19148803621582E-4
23	0.0	0.0	0.0	6.05673604225179E-4	5.19148803621582E-4
24	0.0	0.0	0.0	0.001124822407846761	5.19148803621582E-4
25	0.0	0.0	0.0	0.0012978720090539551	5.19148803621582E-4
26	0.0	0.0	0.0	0.0018170208126755372	6.05673604225179E-4
27	0.0	0.0	0.0	0.002422694416900716	6.05673604225179E-4
28	0.0	0.0	0.0	0.0033744672235402834	6.05673604225179E-4
29	0.0	0.0	0.0	0.004758864033197836	6.05673604225179E-4
30	0.0	0.0	0.0	0.007268083250702149	6.05673604225179E-4
31	0.0	0.0	0.0	0.013065244891143149	6.05673604225179E-4
32	0.0	0.0	0.0	0.018948931332187745	6.05673604225179E-4
33	0.0	0.0	0.0	0.024226944169007163	6.05673604225179E-4
34	0.0	0.0	0.0	0.030197155410655357	6.05673604225179E-4
35	0.0	0.0	0.0	0.03850353626860067	6.05673604225179E-4
36	0.0	0.0	0.0	0.05122268195732943	6.05673604225179E-4
37	0.0	0.0	0.0	0.06939289008408481	6.05673604225179E-4
38	0.0	0.0	0.0	0.09059146623196607	6.05673604225179E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1710	0.0	24.448605	1
GGTATCA	835	0.0	23.980127	1
TGCGAAG	50	0.002581062	21.998983	2
CTCGCGC	55	0.0044846945	19.999075	30
CGCAATA	265	0.0	19.925331	36
GTATATA	235	0.0	19.66292	1
TGTACCG	105	1.7933417E-6	18.856272	5
TCTAGCG	295	0.0	18.643206	28
CTAGCGG	290	0.0	18.206057	29
TAGCGGC	325	0.0	17.599188	30
CTAACAC	140	8.375537E-8	17.284914	3
CAATACG	320	0.0	17.188936	38
GTATAGA	155	1.4357283E-8	17.035154	1
CGCCGGT	300	0.0	16.865887	7
TATCAAC	2540	0.0	16.455933	2
CGGTCCA	335	0.0	16.41715	10
TCAACGC	2525	0.0	16.37944	4
ATCAACG	2540	0.0	16.369322	3
AACGCAG	2545	0.0	16.250723	6
GCACCGT	95	3.3473334E-4	16.209776	6
>>END_MODULE