FastQCFastQC Report
Thu 2 Feb 2017
SRR4062177_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062177_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062889
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39790.19288483287273334No Hit
TATCAACGCAGAGTACTTTTTTTTT23660.11469351962223853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA853.938936E-612.295836
GTATCAA22300.011.8824011
TAATACT2750.011.745954
GGTATCA8450.011.4643221
GTAATAC1104.9694427E-711.2274743
CGTCTTA855.326242E-511.17612715
CGTCAAT600.005886600411.08084219
TCTATAC2150.010.604843
CCCTAAC1002.4066621E-510.4471441
GCTTTAG2053.6379788E-1210.1923351
GGCTAGG1606.200935E-910.0909911
TAGTACT1701.5588739E-910.0592474
GTCTAAG1856.511982E-109.7540891
GTCCTAA1951.6370905E-109.7409261
TAGGACC2350.09.7025374
GTAGGAC2350.09.7023013
AATACTG2450.09.6947575
CCGTGCG900.00111217259.5015549
GACGGCG1106.827175E-59.5013247
TTAGAGT2401.8189894E-129.50040154
Produced by FastQC (version 0.11.5)