##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062177_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2062889
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189575881203496	32.0	32.0	32.0	32.0	32.0
2	30.68866381080126	32.0	32.0	32.0	32.0	32.0
3	30.701401287223888	32.0	32.0	32.0	32.0	32.0
4	30.697466514194414	32.0	32.0	32.0	32.0	32.0
5	30.624384055564793	32.0	32.0	32.0	32.0	32.0
6	34.26730134292247	36.0	36.0	36.0	32.0	36.0
7	34.17430312537417	36.0	36.0	36.0	32.0	36.0
8	34.144077553372966	36.0	36.0	36.0	32.0	36.0
9	34.22998619896659	36.0	36.0	36.0	32.0	36.0
10	33.921218252654405	36.0	36.0	36.0	32.0	36.0
11	34.29971898633421	36.0	36.0	36.0	32.0	36.0
12	34.06409748658314	36.0	36.0	36.0	32.0	36.0
13	34.1380723829542	36.0	36.0	36.0	32.0	36.0
14	34.05700015851556	36.0	36.0	36.0	32.0	36.0
15	33.984723366114224	36.0	36.0	36.0	32.0	36.0
16	34.00850797110266	36.0	36.0	36.0	32.0	36.0
17	33.98006921361256	36.0	36.0	36.0	32.0	36.0
18	33.97222826821996	36.0	36.0	36.0	32.0	36.0
19	33.956796027318966	36.0	36.0	36.0	32.0	36.0
20	33.96563799603372	36.0	36.0	36.0	32.0	36.0
21	33.9628419173305	36.0	36.0	36.0	32.0	36.0
22	33.89826015844769	36.0	36.0	36.0	32.0	36.0
23	33.867688954665034	36.0	36.0	36.0	32.0	36.0
24	33.858113548523455	36.0	36.0	36.0	32.0	36.0
25	33.45452324385849	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	33.0
5	139.0
6	435.0
7	99.0
8	340.0
9	280.0
10	159.0
11	51.0
12	86.0
13	103.0
14	322.0
15	493.0
16	754.0
17	1035.0
18	1462.0
19	2034.0
20	3053.0
21	4457.0
22	6743.0
23	10043.0
24	14219.0
25	19820.0
26	26913.0
27	34616.0
28	45653.0
29	59827.0
30	77450.0
31	103908.0
32	145322.0
33	202005.0
34	427289.0
35	873746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.91636894229884	16.689515093049977	11.212388565445229	26.18172739920596
2	16.708808233893453	19.55069785659716	37.47396853343468	26.266525376074707
3	19.253976110133245	23.579427432384676	28.101060506097536	29.06553595138454
4	12.989098263973561	15.637763919990267	35.434787140537715	35.93835067549846
5	14.784461062948484	36.46040734623654	33.74290693648514	15.012224654329836
6	34.40653827982588	34.89465856803173	16.954600884175356	13.744202267967026
7	30.202159754684942	30.236177371054552	20.820431145384305	18.7412317288762
8	27.892813346358242	33.455284138582314	19.25983188938339	19.392070625676055
9	27.31711318111026	14.667102819250829	18.203530215133558	39.81225378450536
10	15.482169562805165	27.376942631071323	32.31176070561613	24.82912710050739
11	36.5654843590641	21.325128846399362	22.621966581731844	19.48742021280469
12	24.389965687314305	23.42674467313636	29.315770389933647	22.867519249615686
13	29.555627389824192	20.179847769594495	25.001917198385865	25.26260764219545
14	23.29057381743214	19.13797320671133	25.745201687344334	31.826251288512196
15	25.01391889615751	26.949167268969383	22.439541994292647	25.597371840580465
16	25.383013409291326	25.957842923612805	23.665465141618018	24.993678525477847
17	24.108202397541014	25.778540750868416	25.292946236120756	24.82031061546981
18	24.350783437243248	25.72431608478894	25.754938248538412	24.169962229429405
19	25.262556213523073	25.252366451031882	25.51851334276614	23.9665639926789
20	25.492618306754515	24.896590306442043	25.304746244135668	24.306045142667774
21	25.686026200873364	24.644541484716157	25.00601649684619	24.66341581756429
22	25.336159450459974	24.650835752322667	25.085428901123752	24.92757589609361
23	24.61653405783039	24.868474674946633	25.214535222200663	25.30045604502232
24	24.495906553057118	25.08824698189257	25.354722541784795	25.061123923265523
25	24.8322710988111	24.87113452081061	25.26054503887313	25.036049341505162
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	433.0
1	433.0
2	628.0
3	823.0
4	823.0
5	823.0
6	1888.5
7	2954.0
8	2954.0
9	2954.0
10	3327.0
11	3700.0
12	3700.0
13	3700.0
14	4963.5
15	6227.0
16	6227.0
17	6227.0
18	10440.0
19	14653.0
20	14653.0
21	14653.0
22	22563.5
23	30474.0
24	30474.0
25	30474.0
26	42743.5
27	55013.0
28	55013.0
29	55013.0
30	70622.5
31	86232.0
32	86232.0
33	86232.0
34	104966.0
35	123700.0
36	123700.0
37	123700.0
38	147597.0
39	171494.0
40	171494.0
41	171494.0
42	196044.5
43	220595.0
44	220595.0
45	220595.0
46	243408.5
47	266222.0
48	266222.0
49	266222.0
50	274887.5
51	283553.0
52	283553.0
53	283553.0
54	271475.5
55	259398.0
56	259398.0
57	259398.0
58	234455.0
59	209512.0
60	209512.0
61	209512.0
62	180545.5
63	151579.0
64	151579.0
65	151579.0
66	121867.5
67	92156.0
68	92156.0
69	92156.0
70	69407.5
71	46659.0
72	46659.0
73	46659.0
74	33792.5
75	20926.0
76	20926.0
77	20926.0
78	15291.0
79	9656.0
80	9656.0
81	9656.0
82	6744.5
83	3833.0
84	3833.0
85	3833.0
86	2716.0
87	1599.0
88	1599.0
89	1599.0
90	1074.0
91	549.0
92	549.0
93	549.0
94	368.0
95	187.0
96	187.0
97	187.0
98	474.5
99	762.0
100	762.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.210384077863618E-4
2	4.847570567296641E-5
3	2.908542340377984E-4
4	0.009210384077863617
5	0.03213939286117673
6	0.0633577473145671
7	0.10625874683514236
8	0.10771301800533135
9	0.11866852748742177
10	0.1324356278985442
11	0.13951308092679732
12	0.13175696801912268
13	0.12560055339865595
14	0.11503284956194929
15	0.13282343354392795
16	0.11886243031011363
17	0.12254658394125909
18	0.11100936599109307
19	0.09680598422891393
20	0.09268554924671177
21	0.09157060801623354
22	0.1023322146756321
23	0.08187546688164027
24	0.09268554924671177
25	0.0884681628531637
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2062889.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.13556183522371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.29660798733879	42.44978439807972
2	14.424880963725567	16.483473565373675
3	4.882602080001785	8.369106391762017
4	2.1859123756411836	4.9957332681931055
5	1.1769854244255453	3.362386174821139
6	0.6844976516134507	2.346549473989544
7	0.4710119054096903	1.883807089266333
8	0.3372060543350379	1.5413165894937093
9	0.23905601933096213	1.229273997710996
>10	1.2122689958391764	12.082196917169044
>50	0.06578042762476329	2.579525140848677
>100	0.022338273830158954	1.9998416250518298
>500	4.259204419331332E-4	0.1720985608984707
>1k	4.259204419330945E-4	0.5049068073418044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3979	0.19288483287273334	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2366	0.11469351962223853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.847570567296641E-5
2	0.0	0.0	0.0	0.0	4.847570567296641E-5
3	0.0	0.0	0.0	0.0	4.847570567296641E-5
4	0.0	0.0	0.0	0.0	4.847570567296641E-5
5	0.0	0.0	0.0	0.0	4.847570567296641E-5
6	0.0	0.0	0.0	0.0	4.847570567296641E-5
7	0.0	0.0	0.0	0.0	4.847570567296641E-5
8	0.0	0.0	0.0	0.0	4.847570567296641E-5
9	0.0	0.0	0.0	0.0	4.847570567296641E-5
10	0.0	0.0	0.0	0.0	4.847570567296641E-5
11	0.0	0.0	0.0	0.0	4.847570567296641E-5
12	0.0	0.0	0.0	0.0	5.332327624026305E-4
13	0.0	0.0	0.0	0.0	6.301841737485633E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGA	85	3.938936E-6	12.29583	6
GTATCAA	2230	0.0	11.882401	1
TAATACT	275	0.0	11.74595	4
GGTATCA	845	0.0	11.464322	1
GTAATAC	110	4.9694427E-7	11.227474	3
CGTCTTA	85	5.326242E-5	11.176127	15
CGTCAAT	60	0.0058866004	11.080842	19
TCTATAC	215	0.0	10.60484	3
CCCTAAC	100	2.4066621E-5	10.447144	1
GCTTTAG	205	3.6379788E-12	10.192335	1
GGCTAGG	160	6.200935E-9	10.090991	1
TAGTACT	170	1.5588739E-9	10.059247	4
GTCTAAG	185	6.511982E-10	9.754089	1
GTCCTAA	195	1.6370905E-10	9.740926	1
TAGGACC	235	0.0	9.702537	4
GTAGGAC	235	0.0	9.702301	3
AATACTG	245	0.0	9.694757	5
CCGTGCG	90	0.0011121725	9.501554	9
GACGGCG	110	6.827175E-5	9.501324	7
TTAGAGT	240	1.8189894E-12	9.5004015	4
>>END_MODULE