FastQCFastQC Report
Thu 2 Feb 2017
SRR4062177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062889
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22950.025.1200641
GGTATCA10700.019.7419281
TCGAACG808.986564E-519.25165444
TCAACGC30600.018.1170064
CGGATCG1102.7973674E-618.00067526
TATCAAC31300.017.9226912
ATCAACG31550.017.7806723
CAACGCA31300.017.641555
AACGCAG32200.017.2167846
GTATAAT2601.8189894E-1215.2335561
CTATTAG1201.17982134E-414.669351
CGACGGT1503.2542011E-614.6661487
GTACATG53950.014.5605871
GTATAGA3200.014.4401411
TACATGG54150.014.422392
CTAGACA4600.014.34732154
GTAATAC1851.2674536E-714.2697673
ACGCAGA39250.014.1243437
GTGTATA2703.092282E-1113.8543851
GTATATA3500.013.8311011
Produced by FastQC (version 0.11.5)