FastQCFastQC Report
Thu 2 Feb 2017
SRR4062176_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062176_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376563
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27100.1968671248609762No Hit
TATCAACGCAGAGTACTTTTTTTTT17360.12611119142385782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT753.3087417E-916.4561441
CTATACT902.2846507E-914.7785424
CGTTATT801.2929559E-714.2455542
GGTATCA5150.014.1948071
TTATGCT759.664727E-713.9340534
TATACTG1205.0931703E-1113.459035
TCTAATC905.404818E-712.6663993
GTATCAA14800.012.1240041
TAGATTG550.003066947212.0915345
GTCTTAG1201.0102667E-811.8674121
GTTATTC1157.071867E-811.5649743
CAATACT1001.9276558E-611.4005884
TTACCGC1001.9293439E-611.39975916
TCCAACG752.0752904E-411.39893118
GTATAAC1105.0071867E-711.22009851
CGGTTCT1201.2716737E-711.08511612
CCTATAC600.005875993511.08313
TATATCT951.3589877E-511.0005694
GTACTAC951.3694358E-510.992971
TATTCCC1303.2610842E-810.9621065
Produced by FastQC (version 0.11.5)