FastQCFastQC Report
Thu 2 Feb 2017
SRR4062176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376563
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15750.025.845411
GGTATCA6850.022.1641331
GTAGGAC6900.021.9995273
TAGGACG4950.020.8884414
TGTAGGA8050.020.49722
TTAGTAC951.5976675E-518.5259173
ATACGAA1852.1827873E-1117.8387540
TAGGACC6350.017.6689134
CGCGTAA1651.6607373E-917.33296210
CTGTAGG9200.017.2201481
CTAGGAC2300.017.2170243
CGCAATA2055.456968E-1217.17098636
TATAGTG2055.456968E-1217.1703625
TATCAAC23850.016.9729982
TCAACGC24200.016.7269154
AACGCAG24750.016.710756
ATTACAC2650.016.6034163
CAACGCA24950.016.4886255
GATATAC2950.016.4094091
ATCAACG24700.016.3883133
Produced by FastQC (version 0.11.5)