##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1376563
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300584862443635	32.0	32.0	32.0	32.0	32.0
2	31.475898306143634	32.0	32.0	32.0	32.0	32.0
3	31.575374319954843	32.0	32.0	32.0	32.0	32.0
4	31.65462169185137	32.0	32.0	32.0	32.0	32.0
5	31.634172936509263	32.0	32.0	32.0	32.0	32.0
6	35.27269220515153	36.0	36.0	36.0	36.0	36.0
7	35.274984871742156	36.0	36.0	36.0	36.0	36.0
8	35.227707703897316	36.0	36.0	36.0	36.0	36.0
9	35.304262136930895	36.0	36.0	36.0	36.0	36.0
10	35.19763933797436	36.0	36.0	36.0	36.0	36.0
11	35.304001342473974	36.0	36.0	36.0	36.0	36.0
12	35.2350382801223	36.0	36.0	36.0	36.0	36.0
13	35.26279945051552	36.0	36.0	36.0	36.0	36.0
14	35.231053718572994	36.0	36.0	36.0	36.0	36.0
15	35.20922035533427	36.0	36.0	36.0	36.0	36.0
16	35.227977215717694	36.0	36.0	36.0	36.0	36.0
17	35.20873073008645	36.0	36.0	36.0	36.0	36.0
18	35.20161954084194	36.0	36.0	36.0	36.0	36.0
19	35.189014233275195	36.0	36.0	36.0	36.0	36.0
20	35.19145800083251	36.0	36.0	36.0	36.0	36.0
21	35.18172579097361	36.0	36.0	36.0	36.0	36.0
22	35.16446177908312	36.0	36.0	36.0	36.0	36.0
23	35.12985239324317	36.0	36.0	36.0	36.0	36.0
24	35.120640319404195	36.0	36.0	36.0	36.0	36.0
25	35.1071276795904	36.0	36.0	36.0	36.0	36.0
26	35.06576378996094	36.0	36.0	36.0	36.0	36.0
27	35.04680134508918	36.0	36.0	36.0	36.0	36.0
28	35.0252331349891	36.0	36.0	36.0	36.0	36.0
29	35.01314360476055	36.0	36.0	36.0	36.0	36.0
30	34.99300649516223	36.0	36.0	36.0	36.0	36.0
31	34.98300767927076	36.0	36.0	36.0	36.0	36.0
32	34.95362289993266	36.0	36.0	36.0	36.0	36.0
33	34.945804877800725	36.0	36.0	36.0	36.0	36.0
34	34.93006567806922	36.0	36.0	36.0	36.0	36.0
35	34.915894877314	36.0	36.0	36.0	36.0	36.0
36	34.892458972092086	36.0	36.0	36.0	32.0	36.0
37	34.87812835300673	36.0	36.0	36.0	32.0	36.0
38	34.86350788158624	36.0	36.0	36.0	32.0	36.0
39	34.829666350177945	36.0	36.0	36.0	32.0	36.0
40	34.827526237447906	36.0	36.0	36.0	32.0	36.0
41	34.79910763256022	36.0	36.0	36.0	32.0	36.0
42	34.78261873957094	36.0	36.0	36.0	32.0	36.0
43	34.76575935863451	36.0	36.0	36.0	32.0	36.0
44	34.72589558196755	36.0	36.0	36.0	32.0	36.0
45	34.70543084479243	36.0	36.0	36.0	32.0	36.0
46	34.66773115360503	36.0	36.0	36.0	32.0	36.0
47	34.64771172841345	36.0	36.0	36.0	32.0	36.0
48	34.60822061903451	36.0	36.0	36.0	32.0	36.0
49	34.59424087382851	36.0	36.0	36.0	32.0	36.0
50	34.19797858870244	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	11.0
21	41.0
22	97.0
23	248.0
24	657.0
25	1568.0
26	3241.0
27	6364.0
28	10996.0
29	17645.0
30	26132.0
31	38215.0
32	56912.0
33	95376.0
34	215214.0
35	903844.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.778249306029906	17.617466246166817	12.322801459153865	26.28148298864941
2	15.888876377149042	19.66828552436096	37.62409325201509	26.818744846474896
3	18.98632750212668	23.03787923365435	28.415709025185265	29.560084239033706
4	13.057884019837813	15.777774064826675	34.71014403263781	36.454197882697706
5	14.827312411146329	36.3865458922634	33.112081484220454	15.674060212369811
6	35.10782659989161	34.26405784846596	16.484037769457533	14.14407778218489
7	30.783044437486694	30.004583880287356	19.89738210310752	19.31498957911843
8	27.914450700767055	33.768160265821464	18.860306429854646	19.45708260355683
9	27.12589253089034	14.774332885599861	18.437586946619952	39.662187636889854
10	16.69499811850102	26.950983682536638	31.029623075459007	25.324395123503336
11	36.850111473285274	21.23862111650538	22.17057991533987	19.74068749486947
12	24.767409846116742	23.305072125285946	28.60530175516849	23.322216273428822
13	29.74291768702195	19.73894402217697	24.883423424863228	25.634714865937845
14	23.34633431234168	19.46398384963129	25.236767223875695	31.952914614151336
15	25.10164809020728	27.332130821473484	22.086820581404556	25.479400506914686
16	25.508442809446187	25.916998654613398	23.856929903323948	24.717628632616474
17	24.121816437024677	26.12506656070227	25.211268935747945	24.541848066525105
18	24.547223773993636	25.34805889741334	26.123904245573943	23.980813083019083
19	25.6542562890329	24.866133987329313	25.293357441686286	24.186252281951496
20	25.762988899866336	24.690314988086243	25.335401290172605	24.21129482187482
21	25.90306437119115	24.28606609359688	25.018397269140607	24.792472266071368
22	25.70917567884652	24.58209322784355	25.181194031802395	24.527537061507537
23	24.592263485216442	24.56298767292162	25.592072429667223	25.25267641219472
24	24.87152626937276	24.740110392242613	25.53942984833053	24.848933490054094
25	25.009425662938295	24.515368370889483	25.652477441973147	24.82272852419907
26	24.502016632862397	25.136645764199244	25.875884093009198	24.485453509929155
27	24.85345642339961	24.989375636099737	25.28642195396763	24.870745986533027
28	24.753311757419898	24.96071658185226	25.36956924451612	24.91640241621172
29	24.769042673983492	24.92740926066777	25.3042200336638	24.99932803168494
30	24.787693255437887	24.997057853497086	25.39188664962885	24.823362241436175
31	25.183574079078515	24.622536242622893	25.10896759739524	25.084922080903354
32	24.923177508989866	25.00933493153173	25.071083505866117	24.99640405361229
33	24.67246132555546	24.810269113838533	25.54761705852654	24.969652502079466
34	25.185299803204092	24.87241682666777	25.406359217552833	24.53592415257531
35	25.069285180344874	24.944698984495375	25.567993188803662	24.418022646356093
36	24.629348063761793	25.039718544982755	25.424663345830663	24.906270045424794
37	25.257148170668366	24.76853213961662	25.01378455127744	24.960535138437574
38	24.831773046348044	25.071718475652766	25.172694602426475	24.923813875572712
39	25.438066948093034	24.675513363393794	25.026242972513344	24.860176715999824
40	25.639546667383428	24.71521421862162	25.16575727483199	24.479481839162958
41	24.85904969601518	24.928426085813147	25.64994053971199	24.562583678459685
42	25.287650254486383	25.142426650819687	25.233890641323125	24.3360324533708
43	24.93281888960649	24.762459653193638	25.463002986553608	24.841718470646267
44	24.689303256149824	25.04426070992944	25.151488954255818	25.11494707966492
45	24.82159916021024	25.089009723690282	25.202047169160245	24.887343946939236
46	24.989066806534055	24.86869270970301	25.003160092463578	25.139080391299355
47	25.15912970832879	24.943663506592568	25.255675420435146	24.641531364643495
48	25.048928228427624	25.32600657025832	24.703996931360997	24.921068269953057
49	25.091790373598084	25.16545514512832	24.650746164564485	25.09200831670912
50	24.94991057275571	25.56449585845069	24.806144109352594	24.679449459441003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	285.0
1	174.0
2	63.0
3	172.5
4	282.0
5	298.5
6	315.0
7	247.0
8	179.0
9	251.5
10	324.0
11	548.5
12	773.0
13	1416.0
14	2059.0
15	2662.0
16	3265.0
17	3175.0
18	3085.0
19	2907.5
20	2730.0
21	3106.0
22	3482.0
23	4138.5
24	4795.0
25	6124.0
26	7453.0
27	9904.0
28	12355.0
29	15013.5
30	17672.0
31	20390.5
32	23109.0
33	27295.5
34	31482.0
35	36559.5
36	41637.0
37	45996.0
38	50355.0
39	54429.0
40	58503.0
41	62837.5
42	67172.0
43	71066.5
44	74961.0
45	81033.0
46	87105.0
47	91954.5
48	96804.0
49	101307.0
50	105810.0
51	105874.5
52	105939.0
53	106432.5
54	106926.0
55	104621.5
56	102317.0
57	97942.0
58	93567.0
59	86810.0
60	80053.0
61	70266.0
62	60479.0
63	51938.0
64	43397.0
65	36701.0
66	30005.0
67	25228.0
68	20451.0
69	17344.5
70	14238.0
71	11395.5
72	8553.0
73	7324.5
74	6096.0
75	4632.0
76	3168.0
77	2718.5
78	2269.0
79	1867.5
80	1466.0
81	1125.0
82	784.0
83	611.5
84	439.0
85	314.0
86	189.0
87	136.5
88	84.0
89	60.0
90	36.0
91	24.0
92	12.0
93	11.0
94	10.0
95	12.0
96	14.0
97	13.0
98	12.0
99	8.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030728706205237245
2	0.005666286250611123
3	2.905787820826217E-4
4	0.0
5	1.4528939104131085E-4
6	7.264469552065542E-5
7	0.0
8	0.0
9	0.0
10	7.264469552065542E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.085128686445879E-4
17	0.0
18	0.0
19	0.0
20	2.1793408656196629E-4
21	0.0
22	0.0
23	0.0
24	0.0012349598238511421
25	1.4528939104131085E-4
26	7.990916507272097E-4
27	7.264469552065543E-4
28	0.0020340514745783517
29	7.264469552065543E-4
30	0.0012349598238511421
31	5.085128686445879E-4
32	9.443810417685206E-4
33	5.811575641652434E-4
34	7.264469552065543E-4
35	4.3586817312393257E-4
36	1.4528939104131085E-4
37	0.0015981833014544194
38	0.0
39	2.905787820826217E-4
40	1.4528939104131085E-4
41	0.0010170257372891759
42	0.004721905208842603
43	0.004358681731239326
44	0.004358681731239326
45	0.0020340514745783517
46	0.001525538605933764
47	9.443810417685206E-4
48	0.004140747644677359
49	0.004140747644677359
50	0.0018161173880163856
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1376563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.1585380682249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35371898522088	45.933281102229564
2	13.827252323397458	16.63654570552114
3	4.329094642362303	7.812960145305032
4	1.8528490421856831	4.458587585560059
5	0.995407559036205	2.994113176683919
6	0.6107595008988643	2.2045439211212625
7	0.41284746830435404	1.7385410096850427
8	0.28868626715328227	1.3893555033851581
9	0.2166615031031832	1.1730635354116508
>10	1.0386727449592574	10.605671120458721
>50	0.04915156597392735	2.012946299746778
>100	0.02368386549596328	2.561234139563291
>500	0.0010930787178130659	0.38446520990198374
>1k	1.2145319086811845E-4	0.09469154542625827
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1793408656196629E-4
2	0.0	0.0	0.0	0.0	2.1793408656196629E-4
3	0.0	0.0	0.0	0.0	2.1793408656196629E-4
4	0.0	0.0	0.0	0.0	2.1793408656196629E-4
5	0.0	0.0	0.0	0.0	2.1793408656196629E-4
6	7.264469552065542E-5	0.0	0.0	0.0	2.905787820826217E-4
7	7.264469552065542E-5	0.0	0.0	0.0	2.905787820826217E-4
8	7.264469552065542E-5	0.0	0.0	0.0	2.905787820826217E-4
9	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	2.905787820826217E-4
10	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	2.905787820826217E-4
11	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	2.905787820826217E-4
12	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	0.0012349598238511424
13	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	0.001670827996975075
14	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	0.001670827996975075
15	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	0.001670827996975075
16	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	0.0017434726924957303
17	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	0.0017434726924957303
18	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	0.0017434726924957303
19	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	0.0017434726924957303
20	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	0.0018887620835370412
21	7.264469552065542E-5	0.0	0.0	7.264469552065542E-5	0.0019614067790576966
22	7.264469552065542E-5	0.0	0.0	2.905787820826217E-4	0.0021066961700990073
23	7.264469552065542E-5	0.0	0.0	4.3586817312393257E-4	0.0021066961700990073
24	7.264469552065542E-5	0.0	0.0	7.264469552065543E-4	0.0021066961700990073
25	7.264469552065542E-5	0.0	0.0	8.717363462478651E-4	0.0021066961700990073
26	7.264469552065542E-5	0.0	0.0	0.001017025737289176	0.0021066961700990073
27	7.264469552065542E-5	0.0	0.0	0.0012349598238511424	0.002179340865619663
28	7.264469552065542E-5	0.0	0.0	0.0015981833014544194	0.002179340865619663
29	7.264469552065542E-5	0.0	0.0	0.0029784325163468724	0.002179340865619663
30	7.264469552065542E-5	0.0	0.0	0.004431326426759981	0.002179340865619663
31	7.264469552065542E-5	0.0	0.0	0.008572074071437341	0.002179340865619663
32	7.264469552065542E-5	0.0	0.0	0.01256753232507339	0.0022519855611403184
33	7.264469552065542E-5	0.0	0.0	0.01779795040256058	0.0022519855611403184
34	7.264469552065542E-5	0.0	0.0	0.021866053351717284	0.0022519855611403184
35	7.264469552065542E-5	0.0	0.0	0.029203167599303484	0.0022519855611403184
36	7.264469552065542E-5	0.0	0.0	0.038429043930426725	0.0022519855611403184
37	7.264469552065542E-5	0.0	0.0	0.05288533833903715	0.0022519855611403184
38	7.264469552065542E-5	0.0	0.0	0.07249940612961411	0.0024699196477022847
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1575	0.0	25.84541	1
GGTATCA	685	0.0	22.164133	1
GTAGGAC	690	0.0	21.999527	3
TAGGACG	495	0.0	20.888441	4
TGTAGGA	805	0.0	20.4972	2
TTAGTAC	95	1.5976675E-5	18.525917	3
ATACGAA	185	2.1827873E-11	17.83875	40
TAGGACC	635	0.0	17.668913	4
CGCGTAA	165	1.6607373E-9	17.332962	10
CTGTAGG	920	0.0	17.220148	1
CTAGGAC	230	0.0	17.217024	3
CGCAATA	205	5.456968E-12	17.170986	36
TATAGTG	205	5.456968E-12	17.170362	5
TATCAAC	2385	0.0	16.972998	2
TCAACGC	2420	0.0	16.726915	4
AACGCAG	2475	0.0	16.71075	6
ATTACAC	265	0.0	16.603416	3
CAACGCA	2495	0.0	16.488625	5
GATATAC	295	0.0	16.409409	1
ATCAACG	2470	0.0	16.388313	3
>>END_MODULE