FastQCFastQC Report
Thu 2 Feb 2017
SRR4062175_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062175_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416537
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19680.47246703174027754No Hit
CCATAGGGTCTTCTCGTCTTATTAT19080.4580625490652691No Hit
TATCAACGCAGAGTACTTTTTTTTT12420.29817279137267516No Hit
GTATTAGAGGCACTGCCTGCCCAGT11220.26936382602265824No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA10510.2523185215238982No Hit
GTCAGGATACCGCGGCCGTTAAACT9720.23335261933513707No Hit
GTTATATAATTTAAGCTCCATAGGG9030.21678746425887735No Hit
CTTCTACACCATTGGGATGTCCTGA8620.20694440109762158No Hit
CTCTAATACTTGTAATGCTAGAGGT8610.20670432638637146No Hit
ACCTATAACTTCTCTGTTAACCCAA8580.20598410225262104No Hit
ATCTATAACTTTATAGATGCAACAC8560.20550395283012074No Hit
CCATTGGGATGTCCTGATCCAACAT8550.2052638781188706No Hit
CCTATAACTTCTCTGTTAACCCAAC8210.19710133793636578No Hit
CTGTTAGTATGAGTAACAAGAATTC8060.19350021726761368No Hit
GATATACACTGTTCTACAAATCCCG8020.19253991842261312No Hit
ATAAATAATCCACCTATAACTTCTC7980.19157961957761255No Hit
GCCTAAAGGAAAGATCCAAAAAGAT7760.18629797593010944No Hit
GATTAAACCTTGTACCTTTTGCATA7700.1848575276626086No Hit
GACTATAGGCAATAATCACACTATA7690.18461745295135845No Hit
CACTATAAATAATCCACCTATAACT7500.1800560334376058No Hit
GTTATAGATTAACCCAATTTTAAGT7370.17693506219135396No Hit
GGTATCAACGCAGAGTACTTTTTTT7310.1754946139238531No Hit
GTATCAACGCAGAGTACATGGGAGA7180.17237364267760127No Hit
CTATAGAACTAGTACCGCAAGGGAA7160.17189349325510098No Hit
GTCCTACAGTGGACATTTCTAAATT7150.17165341854385086No Hit
GTTCATGCTAGTCCCTAATTAAGGA7000.16805229787509873No Hit
GTCCTACAGTGTGCATTTCTCATTT6950.16685192431884802No Hit
GTACATGGGAGAAATCGTAAATAGA6640.15940960827009365No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6610.15868938413634323No Hit
GATTAAAGATAAGAGACAGTTGGAC6460.1550882634675911No Hit
CTGTTAACCCAACACCGGAATGCCT6450.15484818875634096No Hit
GTCCTAAAGTGTGTATTTCTCATTT6420.15412796462259054No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6410.1538878899113404No Hit
CCTCTAGCATTACAAGTATTAGAGG6340.15220736693258943No Hit
GTACATGGGGTGGTATCAACGCAAA6180.14836617155258716No Hit
CTGTAGGACGTGGAATATGGCAAGA6080.14596542444008576No Hit
TTGTAGAACAGTGTATATCAATGAG6030.14476505088383507No Hit
GTATCCTGACCGTGCAAAGGTAGCA5950.1428444531938339No Hit
GGTCAGGATACCGCGGCCGTTAAAC5880.14116393021508294No Hit
ATCGTAAATAGATAGAAACCGACCT5870.1409238555038328No Hit
ATCCTGACCGTGCAAAGGTAGCATA5680.1363624359900801No Hit
TACTAACAGTGTTGCATCTATAAAG5600.134441838300079No Hit
CTATTAAAGGTTTTTTCCGTTCCAG5600.134441838300079No Hit
CTTTAGGACGTGAAATATGGCGAGG5530.132761315321328No Hit
GATAGAAACCGACCTGGATTGCTCC5380.1291601946525759No Hit
GATATATTTTGATCAACGGACCAAG5250.12603922340632406No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5230.12555907398382377No Hit
ACCTTTGCACGGTCAGGATACCGCG5140.12339840158257248No Hit
CTTTATTGGTGGCTGCTTTTAGGCC5020.1205175050475708No Hit
GTGTATATCAATGAGTTACAATGAG4900.1176366085125691No Hit
GTCCTGATCCAACATCGAGGTCGTA4890.11739653380131897No Hit
GTGTATATCAATGAGTTACAATGAA4830.11595608553381812No Hit
CTGTAGGACCTGGAATATGGCGAGA4760.11427556255506714No Hit
GTATCAACGCAGAGTACATGGGGTG4740.11379541313256684No Hit
ATTTCAGCCTCTTCACTGAAAGGTC4730.11355533842131672No Hit
TGCTAGAGGTGATGTTTTTGGTAAA4710.11307518899881643No Hit
GACCTGGATTGCTCCGGTCTGAACT4690.11259503957631616No Hit
ACCCTATGGAGCTTAAATTATATAA4660.11187481544256572No Hit
GTTAGTATGAGTAACAAGAATTCCA4660.11187481544256572No Hit
GGATTGCTCCGGTCTGAACTCAGAT4660.11187481544256572No Hit
AAATATATCTGGGTCAATAAGATAT4610.110674441886315No Hit
AATCTAAACTTACTTTTTGATTTTG4450.10683324650631278No Hit
GTTCTACAGTGTGGTTTTTATCATT4440.10659317179506263No Hit
CTGTAGGACATGGAATATGGCAAGA4420.10611302237256234No Hit
CTTAAATAGGATTGCGCTGTTATCC4400.10563287295006206No Hit
GCTATCACCAAGCTCGTTAGGCTTT4340.10419242468256121No Hit
TCTAGGAGCTATAGAACTAGTACCG4280.10275197641506037No Hit
GTTATCCCTAGGGTAACTTGGTCCG4260.10227182699256009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCC307.707098E-419.0009634
AAGGTCG250.00603142418.99639118
CTGTAGA1300.017.5224861
GGTTTAC402.7488993E-416.6298438
TCTATAC402.756481E-416.6238423
GTGTAGG350.002179837816.270881
ATAGGAC456.75116E-414.776753
AAGCCAT400.0052603214.2541516
ACCTACT400.0052603214.2541518
AGTGATC400.0052603214.2541518
TAAGCCA400.00526452214.2524365
TGCCATA400.005272935614.24900715
AACGGAC801.2817691E-714.24900715
GACAATT400.00527714614.24729318
GTTTAAA801.2950295E-714.2370191
GTCCTAT400.005302470614.2370191
TAGAGTG954.8494257E-914.0023955
TAGGGTC2850.014.0007094
TGTAGAA2850.013.9956582
TGAACTT759.605192E-713.93571610
Produced by FastQC (version 0.11.5)