##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062175_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 416537 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21517656294639 32.0 32.0 32.0 32.0 32.0 2 30.898981363000164 32.0 32.0 32.0 32.0 32.0 3 30.94629288634623 32.0 32.0 32.0 32.0 32.0 4 30.92671239289667 32.0 32.0 32.0 32.0 32.0 5 30.948842479779707 32.0 32.0 32.0 32.0 32.0 6 34.56267510449252 36.0 36.0 36.0 32.0 36.0 7 34.45451424483299 36.0 36.0 36.0 32.0 36.0 8 34.37189253295626 36.0 36.0 36.0 32.0 36.0 9 34.449640728194616 36.0 36.0 36.0 32.0 36.0 10 34.2388191205103 36.0 36.0 36.0 32.0 36.0 11 34.52594607441836 36.0 36.0 36.0 32.0 36.0 12 34.35872923653841 36.0 36.0 36.0 32.0 36.0 13 34.442167202433396 36.0 36.0 36.0 32.0 36.0 14 34.373323378235305 36.0 36.0 36.0 32.0 36.0 15 34.33303644094042 36.0 36.0 36.0 32.0 36.0 16 34.3340495562219 36.0 36.0 36.0 32.0 36.0 17 34.271704554457344 36.0 36.0 36.0 32.0 36.0 18 34.32467703949469 36.0 36.0 36.0 32.0 36.0 19 34.255936447422435 36.0 36.0 36.0 32.0 36.0 20 34.19950448579598 36.0 36.0 36.0 32.0 36.0 21 34.15162158463714 36.0 36.0 36.0 32.0 36.0 22 34.142455532161605 36.0 36.0 36.0 32.0 36.0 23 34.15377505479705 36.0 36.0 36.0 32.0 36.0 24 34.09713662891892 36.0 36.0 36.0 32.0 36.0 25 33.785733320209246 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 9.0 5 23.0 6 80.0 7 18.0 8 69.0 9 58.0 10 33.0 11 13.0 12 9.0 13 25.0 14 153.0 15 184.0 16 217.0 17 292.0 18 370.0 19 480.0 20 706.0 21 892.0 22 1300.0 23 1750.0 24 2333.0 25 3013.0 26 4076.0 27 5099.0 28 6961.0 29 9456.0 30 12443.0 31 17308.0 32 25331.0 33 37175.0 34 87794.0 35 198867.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.69377456287977 22.57924342128955 12.451114317473046 24.275867698357633 2 13.62307219544049 22.338765436840994 42.50172854207079 21.536433825647723 3 17.119610884105498 27.824859435244182 31.64063437798595 23.41489530266437 4 9.84921968787515 18.91644657863145 42.251140456182476 28.983193277310924 5 10.754116155309216 40.745614877716086 36.81269332001306 11.687575646961633 6 26.864129912558855 42.99293744594984 18.82170654367253 11.321226097818776 7 25.038210866305228 35.12684085054024 22.29351713000346 17.541431153151073 8 23.45547574014838 38.418020711417235 20.33890973061699 17.787593817817392 9 25.1511815717115 16.09951544984329 22.550329763301093 36.198973215144115 10 14.36157553757948 28.325780977679482 34.49042633748813 22.822217147252903 11 31.39995576455202 25.145207666195464 26.655223148602257 16.79961342065026 12 22.059078059193446 28.436364160849248 31.637146868209005 17.8674109117483 13 25.629517804386133 23.463375585646872 28.69327128100713 22.213835328959863 14 19.592564641186737 25.420726519350296 28.575274115164422 26.411434724298548 15 22.63012012567882 31.726208648952714 24.73646632899099 20.90720489637748 16 21.38849896161834 29.702763248980844 27.926938312437503 20.98179947696331 17 18.17121978774804 30.167662223717894 29.406151220508875 22.25496676802519 18 18.111549609917656 29.739031788225905 32.0018939302899 20.14752467156653 19 21.813978747843304 28.488559303705912 28.816809485132623 20.88065246331816 20 21.141184725252547 30.36086735037149 28.956372968349015 19.54157495602695 21 21.94201818990064 27.42253673422801 28.279648697031224 22.35579637884012 22 20.94678054243186 30.452074612016556 28.301524057617165 20.299620787934426 23 20.962243071489496 29.73439208101582 29.367514385593985 19.9358504619007 24 21.573414390926676 30.016459817620415 28.791224634459887 19.61890115699302 25 20.890081794677098 30.002354840880034 29.987456867965513 19.12010649647735 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1180.0 1 1180.0 2 774.5 3 369.0 4 369.0 5 369.0 6 1188.0 7 2007.0 8 2007.0 9 2007.0 10 1932.0 11 1857.0 12 1857.0 13 1857.0 14 2395.0 15 2933.0 16 2933.0 17 2933.0 18 5014.0 19 7095.0 20 7095.0 21 7095.0 22 11268.5 23 15442.0 24 15442.0 25 15442.0 26 23733.5 27 32025.0 28 32025.0 29 32025.0 30 38909.0 31 45793.0 32 45793.0 33 45793.0 34 48652.0 35 51511.0 36 51511.0 37 51511.0 38 50600.5 39 49690.0 40 49690.0 41 49690.0 42 46971.5 43 44253.0 44 44253.0 45 44253.0 46 47542.5 47 50832.0 48 50832.0 49 50832.0 50 45990.0 51 41148.0 52 41148.0 53 41148.0 54 34931.5 55 28715.0 56 28715.0 57 28715.0 58 23213.0 59 17711.0 60 17711.0 61 17711.0 62 14034.5 63 10358.0 64 10358.0 65 10358.0 66 8342.0 67 6326.0 68 6326.0 69 6326.0 70 5013.0 71 3700.0 72 3700.0 73 3700.0 74 2837.5 75 1975.0 76 1975.0 77 1975.0 78 1433.0 79 891.0 80 891.0 81 891.0 82 611.5 83 332.0 84 332.0 85 332.0 86 241.0 87 150.0 88 150.0 89 150.0 90 101.0 91 52.0 92 52.0 93 52.0 94 36.0 95 20.0 96 20.0 97 20.0 98 96.0 99 172.0 100 172.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.602988450005641E-4 2 2.4007471125014104E-4 3 7.202241337504232E-4 4 0.008882764316255218 5 0.030969637751268195 6 0.06169920079128624 7 0.10203175228130994 8 0.10515272352756178 9 0.11547593611131785 10 0.13252124061007786 11 0.13948340723633196 12 0.13084071763132685 13 0.13060064292007673 14 0.11307518899881643 15 0.13348153945507843 16 0.12123772918132122 17 0.12531899927257362 18 0.11307518899881643 19 0.09386921209880515 20 0.08954786729630261 21 0.08930779258505246 22 0.09867070632380798 23 0.07730405702254542 24 0.08930779258505246 25 0.08954786729630261 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 416537.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.488529267893988 #Duplication Level Percentage of deduplicated Percentage of total 1 60.273065933543904 14.75998739179294 2 16.009959344999125 7.841207159956079 3 7.394785985497528 5.432622991070055 4 4.19347925430969 4.1076855781387644 5 2.5990368767444587 3.182329531224623 6 1.714704339731006 2.5194352445572545 7 1.1907098810241759 2.041111363972177 8 0.8623413178489194 1.6893976480846027 9 0.7026484706399788 1.5486144874477263 >10 4.085142308570217 19.368190910651144 >50 0.5199997905475562 8.972969532986179 >100 0.4052205661464641 19.312953083132733 >500 0.04391552933607001 7.441464109863184 >1k 0.004990401060917046 1.7820309671225276 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1968 0.47246703174027754 No Hit CCATAGGGTCTTCTCGTCTTATTAT 1908 0.4580625490652691 No Hit TATCAACGCAGAGTACTTTTTTTTT 1242 0.29817279137267516 No Hit GTATTAGAGGCACTGCCTGCCCAGT 1122 0.26936382602265824 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1051 0.2523185215238982 No Hit GTCAGGATACCGCGGCCGTTAAACT 972 0.23335261933513707 No Hit GTTATATAATTTAAGCTCCATAGGG 903 0.21678746425887735 No Hit CTTCTACACCATTGGGATGTCCTGA 862 0.20694440109762158 No Hit CTCTAATACTTGTAATGCTAGAGGT 861 0.20670432638637146 No Hit ACCTATAACTTCTCTGTTAACCCAA 858 0.20598410225262104 No Hit ATCTATAACTTTATAGATGCAACAC 856 0.20550395283012074 No Hit CCATTGGGATGTCCTGATCCAACAT 855 0.2052638781188706 No Hit CCTATAACTTCTCTGTTAACCCAAC 821 0.19710133793636578 No Hit CTGTTAGTATGAGTAACAAGAATTC 806 0.19350021726761368 No Hit GATATACACTGTTCTACAAATCCCG 802 0.19253991842261312 No Hit ATAAATAATCCACCTATAACTTCTC 798 0.19157961957761255 No Hit GCCTAAAGGAAAGATCCAAAAAGAT 776 0.18629797593010944 No Hit GATTAAACCTTGTACCTTTTGCATA 770 0.1848575276626086 No Hit GACTATAGGCAATAATCACACTATA 769 0.18461745295135845 No Hit CACTATAAATAATCCACCTATAACT 750 0.1800560334376058 No Hit GTTATAGATTAACCCAATTTTAAGT 737 0.17693506219135396 No Hit GGTATCAACGCAGAGTACTTTTTTT 731 0.1754946139238531 No Hit GTATCAACGCAGAGTACATGGGAGA 718 0.17237364267760127 No Hit CTATAGAACTAGTACCGCAAGGGAA 716 0.17189349325510098 No Hit GTCCTACAGTGGACATTTCTAAATT 715 0.17165341854385086 No Hit GTTCATGCTAGTCCCTAATTAAGGA 700 0.16805229787509873 No Hit GTCCTACAGTGTGCATTTCTCATTT 695 0.16685192431884802 No Hit GTACATGGGAGAAATCGTAAATAGA 664 0.15940960827009365 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 661 0.15868938413634323 No Hit GATTAAAGATAAGAGACAGTTGGAC 646 0.1550882634675911 No Hit CTGTTAACCCAACACCGGAATGCCT 645 0.15484818875634096 No Hit GTCCTAAAGTGTGTATTTCTCATTT 642 0.15412796462259054 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 641 0.1538878899113404 No Hit CCTCTAGCATTACAAGTATTAGAGG 634 0.15220736693258943 No Hit GTACATGGGGTGGTATCAACGCAAA 618 0.14836617155258716 No Hit CTGTAGGACGTGGAATATGGCAAGA 608 0.14596542444008576 No Hit TTGTAGAACAGTGTATATCAATGAG 603 0.14476505088383507 No Hit GTATCCTGACCGTGCAAAGGTAGCA 595 0.1428444531938339 No Hit GGTCAGGATACCGCGGCCGTTAAAC 588 0.14116393021508294 No Hit ATCGTAAATAGATAGAAACCGACCT 587 0.1409238555038328 No Hit ATCCTGACCGTGCAAAGGTAGCATA 568 0.1363624359900801 No Hit TACTAACAGTGTTGCATCTATAAAG 560 0.134441838300079 No Hit CTATTAAAGGTTTTTTCCGTTCCAG 560 0.134441838300079 No Hit CTTTAGGACGTGAAATATGGCGAGG 553 0.132761315321328 No Hit GATAGAAACCGACCTGGATTGCTCC 538 0.1291601946525759 No Hit GATATATTTTGATCAACGGACCAAG 525 0.12603922340632406 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 523 0.12555907398382377 No Hit ACCTTTGCACGGTCAGGATACCGCG 514 0.12339840158257248 No Hit CTTTATTGGTGGCTGCTTTTAGGCC 502 0.1205175050475708 No Hit GTGTATATCAATGAGTTACAATGAG 490 0.1176366085125691 No Hit GTCCTGATCCAACATCGAGGTCGTA 489 0.11739653380131897 No Hit GTGTATATCAATGAGTTACAATGAA 483 0.11595608553381812 No Hit CTGTAGGACCTGGAATATGGCGAGA 476 0.11427556255506714 No Hit GTATCAACGCAGAGTACATGGGGTG 474 0.11379541313256684 No Hit ATTTCAGCCTCTTCACTGAAAGGTC 473 0.11355533842131672 No Hit TGCTAGAGGTGATGTTTTTGGTAAA 471 0.11307518899881643 No Hit GACCTGGATTGCTCCGGTCTGAACT 469 0.11259503957631616 No Hit ACCCTATGGAGCTTAAATTATATAA 466 0.11187481544256572 No Hit GTTAGTATGAGTAACAAGAATTCCA 466 0.11187481544256572 No Hit GGATTGCTCCGGTCTGAACTCAGAT 466 0.11187481544256572 No Hit AAATATATCTGGGTCAATAAGATAT 461 0.110674441886315 No Hit AATCTAAACTTACTTTTTGATTTTG 445 0.10683324650631278 No Hit GTTCTACAGTGTGGTTTTTATCATT 444 0.10659317179506263 No Hit CTGTAGGACATGGAATATGGCAAGA 442 0.10611302237256234 No Hit CTTAAATAGGATTGCGCTGTTATCC 440 0.10563287295006206 No Hit GCTATCACCAAGCTCGTTAGGCTTT 434 0.10419242468256121 No Hit TCTAGGAGCTATAGAACTAGTACCG 428 0.10275197641506037 No Hit GTTATCCCTAGGGTAACTTGGTCCG 426 0.10227182699256009 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.4007471125014104E-4 0.0 8 0.0 0.0 0.0 2.4007471125014104E-4 0.0 9 0.0 0.0 0.0 2.4007471125014104E-4 0.0 10 0.0 0.0 0.0 4.801494225002821E-4 0.0 11 0.0 0.0 0.0 4.801494225002821E-4 0.0 12 0.0 0.0 0.0 4.801494225002821E-4 0.0 13 0.0 0.0 0.0 4.801494225002821E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGCC 30 7.707098E-4 19.000963 4 AAGGTCG 25 0.006031424 18.996391 18 CTGTAGA 130 0.0 17.522486 1 GGTTTAC 40 2.7488993E-4 16.629843 8 TCTATAC 40 2.756481E-4 16.623842 3 GTGTAGG 35 0.0021798378 16.27088 1 ATAGGAC 45 6.75116E-4 14.77675 3 AAGCCAT 40 0.00526032 14.254151 6 ACCTACT 40 0.00526032 14.254151 8 AGTGATC 40 0.00526032 14.254151 8 TAAGCCA 40 0.005264522 14.252436 5 TGCCATA 40 0.0052729356 14.249007 15 AACGGAC 80 1.2817691E-7 14.249007 15 GACAATT 40 0.005277146 14.247293 18 GTTTAAA 80 1.2950295E-7 14.237019 1 GTCCTAT 40 0.0053024706 14.237019 1 TAGAGTG 95 4.8494257E-9 14.002395 5 TAGGGTC 285 0.0 14.000709 4 TGTAGAA 285 0.0 13.995658 2 TGAACTT 75 9.605192E-7 13.935716 10 >>END_MODULE