##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062175_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	416537
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17742001310808	32.0	32.0	32.0	32.0	32.0
2	31.464194057190596	32.0	32.0	32.0	32.0	32.0
3	31.60379510103544	32.0	32.0	32.0	32.0	32.0
4	31.68970103496208	32.0	32.0	32.0	32.0	32.0
5	31.67473717820986	32.0	32.0	32.0	32.0	32.0
6	35.302633379507704	36.0	36.0	36.0	36.0	36.0
7	35.32270362536821	36.0	36.0	36.0	36.0	36.0
8	35.27788647827204	36.0	36.0	36.0	36.0	36.0
9	35.34292511829681	36.0	36.0	36.0	36.0	36.0
10	35.248100408847236	36.0	36.0	36.0	36.0	36.0
11	35.362486405769474	36.0	36.0	36.0	36.0	36.0
12	35.28261114858944	36.0	36.0	36.0	36.0	36.0
13	35.325553312190756	36.0	36.0	36.0	36.0	36.0
14	35.28886509481751	36.0	36.0	36.0	36.0	36.0
15	35.2782249836149	36.0	36.0	36.0	36.0	36.0
16	35.284159630477006	36.0	36.0	36.0	36.0	36.0
17	35.287585496606546	36.0	36.0	36.0	36.0	36.0
18	35.277504759481154	36.0	36.0	36.0	36.0	36.0
19	35.230219644353326	36.0	36.0	36.0	36.0	36.0
20	35.20700441977544	36.0	36.0	36.0	36.0	36.0
21	35.185911455645	36.0	36.0	36.0	36.0	36.0
22	35.17250088227456	36.0	36.0	36.0	36.0	36.0
23	35.16994408659975	36.0	36.0	36.0	36.0	36.0
24	35.13441783082895	36.0	36.0	36.0	36.0	36.0
25	35.14388157594644	36.0	36.0	36.0	36.0	36.0
26	35.111173797285716	36.0	36.0	36.0	36.0	36.0
27	35.09641400403806	36.0	36.0	36.0	36.0	36.0
28	35.05088383504947	36.0	36.0	36.0	36.0	36.0
29	35.017242165761985	36.0	36.0	36.0	36.0	36.0
30	34.973860665439084	36.0	36.0	36.0	36.0	36.0
31	34.972614677687694	36.0	36.0	36.0	36.0	36.0
32	34.951272035857556	36.0	36.0	36.0	36.0	36.0
33	34.93419792239345	36.0	36.0	36.0	36.0	36.0
34	34.91088666793106	36.0	36.0	36.0	36.0	36.0
35	34.88648787502671	36.0	36.0	36.0	36.0	36.0
36	34.82116834758977	36.0	36.0	36.0	32.0	36.0
37	34.77863911249181	36.0	36.0	36.0	32.0	36.0
38	34.7606047001827	36.0	36.0	36.0	32.0	36.0
39	34.66878572611797	36.0	36.0	36.0	32.0	36.0
40	34.68354551936563	36.0	36.0	36.0	32.0	36.0
41	34.65959806691843	36.0	36.0	36.0	32.0	36.0
42	34.642747222935775	36.0	36.0	36.0	32.0	36.0
43	34.655711257343285	36.0	36.0	36.0	32.0	36.0
44	34.53291304253884	36.0	36.0	36.0	32.0	36.0
45	34.49023256037279	36.0	36.0	36.0	32.0	36.0
46	34.352225612610646	36.0	36.0	36.0	32.0	36.0
47	34.34531386167375	36.0	36.0	36.0	32.0	36.0
48	34.26186869353743	36.0	36.0	36.0	32.0	36.0
49	34.196563570583166	36.0	36.0	36.0	32.0	36.0
50	33.83402674912433	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	9.0
22	29.0
23	60.0
24	165.0
25	412.0
26	929.0
27	2026.0
28	3521.0
29	5723.0
30	8484.0
31	12319.0
32	18354.0
33	30410.0
34	66918.0
35	267173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.24625179215128	23.119171755936012	12.937591108528117	24.69698534338459
2	13.350603702645536	22.447078482544363	42.165790743626246	22.036527071183855
3	16.80599035857645	27.954366489331058	32.08078053277508	23.158862619317418
4	9.741031408974472	19.025680791862428	42.6327072985113	28.6005805006518
5	10.490544657497413	40.959626635809066	36.65196609184778	11.89786261484574
6	26.94587489646728	43.13971214903909	18.748244445244698	11.166168509248923
7	24.840770447763344	35.120529508783136	22.51780754170698	17.520892501746545
8	23.583499184946355	38.29503741564375	20.250061819238148	17.87140158017175
9	25.258500445338587	15.666795506761705	22.751160161042115	36.32354388685759
10	14.393439238290956	28.180929905386552	34.8057915623342	22.61983929398829
11	31.586149609758557	24.760105344783295	26.852596528039523	16.80114851741862
12	22.07631014771797	28.070975687633993	31.93425794107126	17.918456223576776
13	25.59316459282129	23.258918175336166	29.066325440477076	22.081591791365472
14	19.734861488895344	25.082045532569737	28.746545925091887	26.436547053443032
15	22.616718322742038	31.402252380940947	25.03883208454471	20.942197211772303
16	21.382990982685605	29.305071399076184	28.16302228880374	21.148915329434473
17	18.09539128576813	29.695801333374945	29.77094471799624	22.437862662860685
18	18.098119005605774	29.170417852041247	32.67360485913549	20.0578582832175
19	21.85712193634659	27.890679579485134	29.474452449602317	20.777746034565958
20	21.232978662108437	29.819031248199433	29.530460752491983	19.417529337200147
21	21.83935640771408	26.82834898220326	29.08625164151084	22.246042968571818
22	20.86945457426351	29.798313235078755	28.978938245582025	20.353293945075706
23	20.947238780708556	29.069686486434577	29.917390291858826	20.065684440998037
24	21.554114559950833	29.380145150564303	29.391428770349528	19.674311519135337
25	21.034676474542415	29.186913015921796	30.473236406937215	19.305174102598574
26	18.690040621128748	29.318275666695477	32.40159219459729	19.590091517578482
27	19.033934723372585	30.649045098251047	29.953785396185197	20.36323478219117
28	18.56459651784771	29.207920792079207	31.027503817307046	21.199978872766035
29	19.913956190640818	28.358445449569302	29.409265074472067	22.318333285317816
30	20.68076402293221	28.233124945982542	30.353730325641248	20.732380705444
31	20.995885089812596	27.748995280097184	30.30412883462094	20.950990795469277
32	18.976258669822894	29.4993649932418	30.453435638643946	21.070940698291363
33	20.216259819079262	29.709316841761574	30.364482301649797	19.709941037509363
34	19.748160418692308	29.284934039156376	31.82325614894307	19.143649393208253
35	20.73309213827343	29.75533986176498	29.474932599024818	20.036635400936774
36	19.33753784177636	28.77727548813191	30.58863918451422	21.29654748557751
37	20.96655703070607	28.316567834249636	28.303843660720716	22.413031474323578
38	20.176598957595605	30.187714416726486	29.333528594098485	20.302158031579427
39	20.39545391252575	29.404322336231857	28.238751218387936	21.96147253285446
40	21.35613728465247	29.289185088443737	30.557262757600785	18.797414869303015
41	21.286908840355988	28.305560423783948	30.89359066388497	19.51394007197509
42	20.052290280682126	31.065180387063318	29.097212385449957	19.785316946804603
43	18.407216049323434	31.321005675652312	30.159465662783663	20.112312612240586
44	19.607894920064055	29.835470480479977	29.756722950343438	20.79991164911253
45	19.362056630318396	30.05550673907511	30.240849310727302	20.341587319879192
46	21.86412885599253	29.317187121123002	27.73481671056138	21.08386731232309
47	21.972516049110034	29.949776008681162	27.58838414151066	20.48932380069814
48	20.422156493681097	30.4743018476549	27.57509699227903	21.52844466638497
49	20.451926401106288	29.322398494218877	29.156263204394424	21.069411900280414
50	19.288073905486254	30.081482335090705	28.5709141194434	22.05952963997964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	459.0
1	258.5
2	58.0
3	135.5
4	213.0
5	213.0
6	213.0
7	162.0
8	111.0
9	176.0
10	241.0
11	373.0
12	505.0
13	916.0
14	1327.0
15	1879.0
16	2431.0
17	2964.0
18	3497.0
19	3539.5
20	3582.0
21	4314.0
22	5046.0
23	4658.5
24	4271.0
25	6212.5
26	8154.0
27	11748.0
28	15342.0
29	17241.5
30	19141.0
31	19189.0
32	19237.0
33	22072.0
34	24907.0
35	28139.0
36	31371.0
37	36061.0
38	40751.0
39	38855.5
40	36960.0
41	36535.5
42	36111.0
43	29943.5
44	23776.0
45	22618.0
46	21460.0
47	20481.5
48	19503.0
49	17388.0
50	15273.0
51	15545.5
52	15818.0
53	14950.5
54	14083.0
55	12947.5
56	11812.0
57	11525.0
58	11238.0
59	9862.0
60	8486.0
61	7360.5
62	6235.0
63	5186.0
64	4137.0
65	3822.5
66	3508.0
67	2994.0
68	2480.0
69	2085.5
70	1691.0
71	1400.5
72	1110.0
73	942.5
74	775.0
75	625.5
76	476.0
77	415.0
78	354.0
79	284.5
80	215.0
81	152.0
82	89.0
83	67.5
84	46.0
85	34.5
86	23.0
87	14.5
88	6.0
89	5.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	3.5
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033130310152519465
2	0.005761793070003385
3	2.4007471125014104E-4
4	0.0
5	0.0
6	4.801494225002821E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0012003735562507052
17	0.0
18	4.801494225002821E-4
19	0.0
20	2.4007471125014104E-4
21	0.0
22	0.0
23	0.0
24	9.602988450005641E-4
25	2.4007471125014104E-4
26	0.0012003735562507052
27	4.801494225002821E-4
28	0.0031209712462518338
29	0.0012003735562507052
30	0.0012003735562507052
31	7.202241337504232E-4
32	0.0014404482675008463
33	2.4007471125014104E-4
34	4.801494225002821E-4
35	0.0
36	0.0
37	0.0016805229787509874
38	0.0
39	7.202241337504232E-4
40	2.4007471125014104E-4
41	9.602988450005641E-4
42	0.0038411953800022566
43	0.005041568936252961
44	0.0038411953800022566
45	0.0026408218237515516
46	0.0024007471125014105
47	7.202241337504232E-4
48	0.0021606724012512694
49	0.0021606724012512694
50	0.0012003735562507052
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	416537.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.39257161353786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.4310711419485	18.937948726766734
2	14.941375829582201	8.78330918151557
3	6.692963557324455	5.901702319963745
4	3.7005449325489748	4.350741277562415
5	2.305051803698067	3.3875700106555025
6	1.5226744042932465	2.685318988337421
7	1.026558258790741	2.112123099588291
8	0.7218892778362362	1.6974545836677364
9	0.6034687529045192	1.5963748682650616
>10	3.320281880267305	18.623532291842267
>50	0.4042152164614639	8.330568371471198
>100	0.30132407045309123	17.304866844713914
>500	0.026947681097430926	5.572753729653633
>1k	0.0016331927937836925	0.7157357059965171
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1884	0.4523007559952657	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	1098	0.2636020329526549	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	906	0.2175076883926278	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	881	0.21150582061137427	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	873	0.20958522292137313	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	865	0.207664625231372	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	853	0.2047837286963703	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	853	0.2047837286963703	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	806	0.19350021726761368	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	800	0.19205976900011282	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	792	0.1901391713101117	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	791	0.18989909659886156	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	772	0.18533767708510887	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	770	0.1848575276626086	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	766	0.18389722881760803	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	762	0.18293692997260746	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	721	0.1730938668113517	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	683	0.16397102778384634	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	678	0.16277065422759562	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC	672	0.16133020596009476	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	664	0.15940960827009365	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	651	0.15628863702384183	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA	639	0.15340774048884012	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	636	0.1526875163550897	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	628	0.1507669186650886	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC	618	0.14836617155258716	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT	609	0.1462054991513359	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	603	0.14476505088383507	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	578	0.13876318310258154	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT	575	0.13804295896883112	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	573	0.13756280954633082	No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT	560	0.134441838300079	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA	560	0.134441838300079	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA	558	0.1339616888775787	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	522	0.12531899927257362	No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA	491	0.11787668322381925	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	482	0.115716010822568	No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAATTTATTAAACCT	478	0.11475571197756743	No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA	462	0.11091451659756515	No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT	462	0.11091451659756515	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT	453	0.1087538441963139	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	441	0.10587294766131221	No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA	436	0.1046725741050615	No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA	423	0.10155160285880967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	2.4007471125014104E-4	0.0
19	0.0	0.0	0.0	2.4007471125014104E-4	0.0
20	0.0	0.0	0.0	2.4007471125014104E-4	0.0
21	0.0	0.0	0.0	9.602988450005641E-4	0.0
22	0.0	0.0	0.0	0.0019205976900011283	0.0
23	0.0	0.0	0.0	0.0036011206687521155	0.0
24	0.0	0.0	0.0	0.0038411953800022566	0.0
25	0.0	0.0	0.0	0.004321344802502539	0.0
26	0.0	0.0	0.0	0.005281643647503103	0.0
27	0.0	0.0	0.0	0.005761793070003385	0.0
28	0.0	0.0	0.0	0.00696216662625409	0.0
29	0.0	0.0	0.0	0.009602988450005642	0.0
30	0.0	0.0	0.0	0.015124706808758886	0.0
31	0.0	0.0	0.0	0.02496776997001467	0.0
32	0.0	0.0	0.0	0.038171879088772426	0.0
33	0.0	0.0	0.0	0.04489397100377637	0.0
34	0.0	0.0	0.0	0.054256884742531875	0.0
35	0.0	0.0	0.0	0.06482017203753808	0.0
36	0.0	0.0	0.0	0.08090517769129753	0.0
37	0.0	0.0	0.0	0.1017916775700598	0.0
38	0.0	0.0	0.0	0.12411862571632291	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTCGG	30	1.299963E-4	36.665268	40
CCCGTGT	25	0.002351469	35.198654	44
AGGGGTC	35	3.2149896E-4	31.42737	5
CCTAGAG	65	1.7418643E-8	30.460375	3
ACGATTA	65	1.7418643E-8	30.460375	40
ACATATA	45	4.0609484E-5	29.335733	2
CGCTCTA	30	0.005741819	29.332214	15
TAGGACC	385	0.0	28.57034	4
TTAGGAC	245	0.0	27.83567	3
GAGAGTT	40	7.0259103E-4	27.498951	39
TAGGGCG	40	7.0259103E-4	27.498951	31
GAGTTAA	40	7.0259103E-4	27.498951	41
CTCTAGG	65	5.880338E-7	27.095406	1
TCCTACA	475	0.0	26.865358	2
GTCCTAC	460	0.0	26.80089	1
TACCCTT	50	8.32391E-5	26.40216	27
AGAGTTA	50	8.3307124E-5	26.398993	40
CTAGGAC	125	0.0	26.398989	3
CTGTAGG	645	0.0	25.940174	1
AGGACCT	450	0.0	25.910122	5
>>END_MODULE