FastQCFastQC Report
Thu 2 Feb 2017
SRR4062174_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062174_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281826
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTAT16390.5815645114361344No Hit
GTATCAACGCAGAGTACTTTTTTTT11010.39066658150773886No Hit
GTATTAGAGGCACTGCCTGCCCAGT8350.29628210314165476No Hit
GTCAGGATACCGCGGCCGTTAAACT7820.27747617324164553No Hit
TATCAACGCAGAGTACTTTTTTTTT7230.2565412701454089No Hit
CCATTGGGATGTCCTGATCCAACAT7050.25015435055672647No Hit
GTTATATAATTTAAGCTCCATAGGG6870.2437674309680441No Hit
ACCTATAACTTCTCTGTTAACCCAA6800.24128362890577876No Hit
CTGTTAGTATGAGTAACAAGAATTC6770.24021914230766503No Hit
CCTATAACTTCTCTGTTAACCCAAC6590.23383222271898263No Hit
ATAAATAATCCACCTATAACTTCTC6540.23205807838879308No Hit
CACTATAAATAATCCACCTATAACT6390.22673564539822444No Hit
GACTATAGGCAATAATCACACTATA6370.22602598766614862No Hit
GATTAAACCTTGTACCTTTTGCATA6280.22283252787180743No Hit
GTTCATGCTAGTCCCTAATTAAGGA6190.21963906807746622No Hit
ATCTATAACTTTATAGATGCAACAC6170.21892941034539043No Hit
CTTCTACACCATTGGGATGTCCTGA6140.2178649237472767No Hit
CTCTAATACTTGTAATGCTAGAGGT5950.21112317529255642No Hit
CTGTTAACCCAACACCGGAATGCCT5810.20615557116802566No Hit
GCCTAAAGGAAAGATCCAAAAAGAT5800.20580074230198775No Hit
CTATAGAACTAGTACCGCAAGGGAA5600.19870416498122956No Hit
ATCCTGACCGTGCAAAGGTAGCATA5460.19373656085669883No Hit
GTTATAGATTAACCCAATTTTAAGT5390.19125275879443346No Hit
GTACATGGGAGAAATCGTAAATAGA5340.18947861446424388No Hit
GTATCAACGCAGAGTACATGGGAGA5310.18841412786613018No Hit
GATTAAAGATAAGAGACAGTTGGAC5180.18380135260763733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA5080.18025306394725824No Hit
CCTCTAGCATTACAAGTATTAGAGG5000.17741443301895496No Hit
GGTCAGGATACCGCGGCCGTTAAAC4930.1749306309566896No Hit
GTATCCTGACCGTGCAAAGGTAGCA4760.1688985402340451No Hit
ATCGTAAATAGATAGAAACCGACCT4600.16322127837743855No Hit
TACTAACAGTGTTGCATCTATAAAG4530.16073747631517318No Hit
GTATAGGGGTCCTAGGAAGATAATA4520.16038264744913527No Hit
CTATTAAAGGTTTTTTCCGTTCCAG4510.16002781858309736No Hit
CATTTACACCTACTACCCAACTATC4390.15576987219064245No Hit
GATATATTTTGATCAACGGACCAAG4360.15470538559252872No Hit
CTTTATTGGTGGCTGCTTTTAGGCC4330.153640898994415No Hit
GTTAGTATGAGTAACAAGAATTCCA4320.15328607012837708No Hit
GGTATCAACGCAGAGTACTTTTTTT4310.15293124126233917No Hit
ACCTTTGCACGGTCAGGATACCGCG4250.15080226806611172No Hit
GATAGAAACCGACCTGGATTGCTCC4000.14193154641516395No Hit
GACCTGGATTGCTCCGGTCTGAACT3970.14086705981705025No Hit
GCTATCACCAAGCTCGTTAGGCTTT3950.1401574020849744No Hit
TGCTAGAGGTGATGTTTTTGGTAAA3830.13589945569251952No Hit
TATCAACGCAGAGTACATGGGAGAA3760.13341565363025412No Hit
GTCCTGATCCAACATCGAGGTCGTA3760.13341565363025412No Hit
GGATACCGCGGCCGTTAAACTTTAG3700.13128668043402666No Hit
GTTTAAAATTGAACTTAAATTCATT3600.12773839177364757No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3600.12773839177364757No Hit
ACCTTGTACCTTTTGCATAATGAAC3460.12277078764911682No Hit
ACCCTATGGAGCTTAAATTATATAA3450.12241595878307893No Hit
ATTTCAGCCTCTTCACTGAAAGGTC3440.12206112991704102No Hit
AGCATGAACGGCTAAACGAGGGTCC3360.11922249898873774No Hit
CTTAAATAGGATTGCGCTGTTATCC3290.11673869692647237No Hit
CTATAAATAATCCACCTATAACTTC3290.11673869692647237No Hit
CAGTTGGACCCTCGTTTAGCCGTTC3290.11673869692647237No Hit
AATCTAAACTTACTTTTTGATTTTG3280.11638386806043445No Hit
GTATCAACGCAGAGTACATGGGGTG3280.11638386806043445No Hit
TCTAGGAGCTATAGAACTAGTACCG3270.11602903919439654No Hit
ACTAAAAGTATTGGAGAAAGAAATT3260.11567421032835865No Hit
AAATATATCTGGGTCAATAAGATAT3260.11567421032835865No Hit
GTATTGGAATTAGTGAAATTGGAGT3250.11531938146232072No Hit
GATATAGGCTTACTAGGAGGGTGAA3050.10822280414156252No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3050.10822280414156252No Hit
ATGCAACACTGTTAGTATGAGTAAC3050.10822280414156252No Hit
GGATTGCTCCGGTCTGAACTCAGAT3040.10786797527552461No Hit
GTTATCCCTAGGGTAACTTGGTCCG3010.10680348867741088No Hit
GTATAAGTTTGAAATTTCGGTTGGG2990.10609383094533507No Hit
CATTTATCCTACTAAAAGTATTGGA2960.10502934434722133No Hit
GTTATACGCGTATGCCTGGAGAATT2950.10467451548118344No Hit
GTGTAAATGTATGTGGTAAAAGGCC2880.10219071341891804No Hit
TCCTAACACTCCTCGTCCCCATTCT2880.10219071341891804No Hit
GGGCAGGCAGTGCCTCTAATACTTG2840.10077139795476643No Hit
ATTATAACCTAGACTTACAAGTCAA2840.10077139795476643No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2830.10041656908872851No Hit
TTCCAGAAGAGCTGTCCCTCTTTTG2820.1000617402226906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAA250.006013258419.003739
AGTTCCC307.700312E-419.0003578
ACGGAAG307.7095197E-418.99698319
GTATAAA250.006031818.9936081
AACTTGC453.5217407E-516.8892047
TCCAACG453.5271125E-516.88620618
CTAACAC402.7543915E-416.6223583
GTCCTAA402.7581272E-416.6194081
CCCAATT753.2359821E-916.469913
GATCGTA350.00216143116.28891212
TTCCCCT350.00216143116.28891210
GTTCCCC350.00216143116.2889129
CGTAACG350.00216143116.28891215
TCCCCTA350.00216143116.28891211
AGAACAG350.002163998316.2860185
TAGATAC350.002163998316.2860185
TAACGGA350.002166568316.28312717
TATGAAC551.1196884E-515.54850614
ACTCTTA551.1234672E-515.54298419
TAACTTT1200.015.0419486
Produced by FastQC (version 0.11.5)