FastQCFastQC Report
Thu 2 Feb 2017
SRR4062173_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062173_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753139
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT22710.3015379631117231No Hit
GTCCTACAGTGTGCATTTCTCATTT20020.26582078474225873No Hit
GTATCAACGCAGAGTACTTTTTTTT18860.2504185814305195No Hit
GTCCTACAGTGGACATTTCTAAATT17740.2355474885778057No Hit
CTTTAGGACGTGAAATATGGCGAGG16440.21828639865947722No Hit
CTGTAGGACGTGGAATATGGCAAGA16420.21802084342996447No Hit
CTGTAGGACCTGGAATATGGCGAGA14580.19358976231479183No Hit
TATCAACGCAGAGTACTTTTTTTTT11720.15561536449446914No Hit
CTGAAGGACCTGGAATATGGCGAGA8220.10914319932973861No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7730.10263709620667633No Hit
ATTTAGAAATGTCCACTGTAGGACG7570.10051265437057434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG250.00603863818.9948833
TAGCACC350.00216981316.2845754
TCCAACG1350.014.07308918
CCAACGA802.0000462E-613.05984919
TAGGACC6050.012.7181184
CTAGGAC1850.012.3210053
TTAGACT701.0901691E-412.2134314
GTATCAA8250.011.9693481
GTCTATA658.051053E-411.686051
GTCCTAA5950.011.3300681
GTCCTAC11950.011.2032061
GGGTAAA855.343224E-511.1704891
TAGAAAT2500.011.0192294
GGCGAGG5100.010.98796619
AGGACCT8500.010.9529045
TGTAGGA12750.010.8747792
GTATAGG700.001496520910.8513331
GTTATGA700.001496520910.8513331
CTGTAGG12300.010.8072211
AAATGTC2550.010.8053217
Produced by FastQC (version 0.11.5)