##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062173_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	753139
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.279763762067827	32.0	32.0	32.0	32.0	32.0
2	30.870525892298765	32.0	32.0	32.0	32.0	32.0
3	30.883754526056943	32.0	32.0	32.0	32.0	32.0
4	30.882258122338637	32.0	32.0	32.0	32.0	32.0
5	30.85951995581161	32.0	32.0	32.0	32.0	32.0
6	34.50294036027878	36.0	36.0	36.0	32.0	36.0
7	34.403655898844704	36.0	36.0	36.0	32.0	36.0
8	34.38581988185448	36.0	36.0	36.0	32.0	36.0
9	34.417747587098795	36.0	36.0	36.0	32.0	36.0
10	34.172784837858615	36.0	36.0	36.0	32.0	36.0
11	34.49654711812826	36.0	36.0	36.0	32.0	36.0
12	34.27123811142432	36.0	36.0	36.0	32.0	36.0
13	34.32954872872073	36.0	36.0	36.0	32.0	36.0
14	34.29264982957993	36.0	36.0	36.0	32.0	36.0
15	34.234501200973526	36.0	36.0	36.0	32.0	36.0
16	34.23966625018755	36.0	36.0	36.0	32.0	36.0
17	34.21453941437105	36.0	36.0	36.0	32.0	36.0
18	34.221131822943704	36.0	36.0	36.0	32.0	36.0
19	34.1598788537043	36.0	36.0	36.0	32.0	36.0
20	34.15843157770345	36.0	36.0	36.0	32.0	36.0
21	34.13276964809949	36.0	36.0	36.0	32.0	36.0
22	34.055588676193906	36.0	36.0	36.0	32.0	36.0
23	34.05505623795873	36.0	36.0	36.0	32.0	36.0
24	34.02773458817031	36.0	36.0	36.0	32.0	36.0
25	33.65254089882478	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	7.0
5	60.0
6	174.0
7	37.0
8	134.0
9	95.0
10	59.0
11	10.0
12	33.0
13	45.0
14	148.0
15	209.0
16	306.0
17	335.0
18	542.0
19	692.0
20	1051.0
21	1439.0
22	2071.0
23	3073.0
24	4311.0
25	5972.0
26	8268.0
27	10494.0
28	14222.0
29	18832.0
30	24437.0
31	33642.0
32	48383.0
33	69557.0
34	156279.0
35	348222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53215248363496	17.761077104882293	11.401351692270921	26.305418719211822
2	14.745106474510647	20.06710589559948	41.69262472481803	23.495162905071844
3	19.513260588421183	24.27854804058773	28.710030353164246	27.49816101782684
4	12.052957267681194	16.35102116697209	37.688099221841554	33.90792234350516
5	13.05795047465223	37.93379175870373	35.29667258626026	13.711585180383787
6	32.23978082890678	36.97728305436495	17.734842304697775	13.048093812030496
7	28.438717052357344	30.924062578921486	21.66376457139819	18.973455797322984
8	25.105310521481485	37.72130495986303	19.752917390558807	17.420467128096675
9	26.795026712874154	14.824415527099044	19.20920853057258	39.171349229454215
10	15.048461078242372	28.290500565046866	34.02220301801503	22.63883533869574
11	35.48490892168595	20.9799228825954	24.972211142135354	18.5629570535833
12	24.820845000013296	23.475487341486048	31.651735975387624	20.05193168311303
13	30.247534822255933	21.440033396394732	25.0667063736328	23.245725407716535
14	21.12766862520937	21.948129636030096	26.62005157791189	30.304150160848643
15	22.776694950182545	31.9745102679533	22.617949174761286	22.630845607102877
16	22.30208845698789	26.8193238770057	28.169974608829744	22.70861305717666
17	21.683548325771042	28.077750908339173	27.790326204438465	22.448374561451324
18	21.753295953932415	26.35832648753267	30.5698896430347	21.318487915500224
19	24.652345364627013	25.356625939162598	26.439561987721937	23.551466708488448
20	24.633919434365986	26.67822920404556	27.35045120476323	21.337400156825222
21	23.556664243442345	25.64028275971139	25.89824318451546	24.9048098123308
22	23.968498704060607	28.00292417093108	26.210806140758958	21.81777098424935
23	22.179329077388388	27.881001865741062	27.23862349770636	22.70104555916419
24	23.963829385700276	26.166946203919544	27.84775807579137	22.021466334588816
25	23.05213229831577	27.039102878459857	27.774300253438426	22.13446456978594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	797.0
1	797.0
2	598.5
3	400.0
4	400.0
5	400.0
6	890.0
7	1380.0
8	1380.0
9	1380.0
10	1520.5
11	1661.0
12	1661.0
13	1661.0
14	2131.5
15	2602.0
16	2602.0
17	2602.0
18	4655.0
19	6708.0
20	6708.0
21	6708.0
22	11133.5
23	15559.0
24	15559.0
25	15559.0
26	24604.0
27	33649.0
28	33649.0
29	33649.0
30	45006.5
31	56364.0
32	56364.0
33	56364.0
34	61705.0
35	67046.0
36	67046.0
37	67046.0
38	71990.5
39	76935.0
40	76935.0
41	76935.0
42	81983.5
43	87032.0
44	87032.0
45	87032.0
46	91288.5
47	95545.0
48	95545.0
49	95545.0
50	93710.0
51	91875.0
52	91875.0
53	91875.0
54	83137.0
55	74399.0
56	74399.0
57	74399.0
58	65552.0
59	56705.0
60	56705.0
61	56705.0
62	48097.5
63	39490.0
64	39490.0
65	39490.0
66	31578.5
67	23667.0
68	23667.0
69	23667.0
70	17560.0
71	11453.0
72	11453.0
73	11453.0
74	8515.5
75	5578.0
76	5578.0
77	5578.0
78	4006.5
79	2435.0
80	2435.0
81	2435.0
82	1692.0
83	949.0
84	949.0
85	949.0
86	685.5
87	422.0
88	422.0
89	422.0
90	274.5
91	127.0
92	127.0
93	127.0
94	92.5
95	58.0
96	58.0
97	58.0
98	180.5
99	303.0
100	303.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001194998532807357
2	1.32777614756373E-4
3	6.63888073781865E-4
4	0.010489431565753465
5	0.03611551121373346
6	0.0643971431568409
7	0.10741709033790575
8	0.1115331963953533
9	0.11684430098560825
10	0.13131706099405288
11	0.13795594173187153
12	0.13237928191210385
13	0.1277320653956308
14	0.11671152337085187
15	0.13184817145307837
16	0.12069485181354306
17	0.12507651310050336
18	0.11325930538718618
19	0.09825543491971601
20	0.09413932886226846
21	0.09267877509994835
22	0.10476153804277828
23	0.08272045399322038
24	0.09360821840324296
25	0.09135099895238462
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	753139.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.43717206845538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.54756547630429	47.794135170653654
2	14.690905433833535	18.34517180827341
3	4.522060421904724	8.470339939992517
4	1.7805006991933345	4.446777140941573
5	0.8337331050302608	2.6027968668970987
6	0.45093612068340605	1.6893105695394948
7	0.2650980044970729	1.158637880525153
8	0.17170579363502342	0.8576659345872518
9	0.1270523325114932	0.7139509512046837
>10	0.537983838671246	5.998884048881769
>50	0.04103909811872546	1.737461152107775
>100	0.026717411246544587	3.1316026572970954
>500	0.0029923500538554496	1.2050784950504778
>1k	0.0017099143164888283	1.8481873840480691
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	2271	0.3015379631117231	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2002	0.26582078474225873	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1886	0.2504185814305195	No Hit
GTCCTACAGTGGACATTTCTAAATT	1774	0.2355474885778057	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1644	0.21828639865947722	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1642	0.21802084342996447	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1458	0.19358976231479183	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1172	0.15561536449446914	No Hit
CTGAAGGACCTGGAATATGGCGAGA	822	0.10914319932973861	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	773	0.10263709620667633	No Hit
ATTTAGAAATGTCCACTGTAGGACG	757	0.10051265437057434	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.32777614756373E-4
2	0.0	0.0	0.0	0.0	1.32777614756373E-4
3	0.0	0.0	0.0	0.0	1.32777614756373E-4
4	0.0	0.0	0.0	0.0	1.32777614756373E-4
5	0.0	0.0	0.0	0.0	1.32777614756373E-4
6	0.0	0.0	0.0	0.0	1.32777614756373E-4
7	0.0	0.0	0.0	0.0	1.32777614756373E-4
8	0.0	0.0	0.0	0.0	1.32777614756373E-4
9	0.0	0.0	0.0	0.0	2.65555229512746E-4
10	0.0	0.0	0.0	0.0	2.65555229512746E-4
11	0.0	0.0	0.0	0.0	2.65555229512746E-4
12	0.0	0.0	0.0	0.0	2.65555229512746E-4
13	0.0	0.0	0.0	0.0	2.65555229512746E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGGG	25	0.006038638	18.994883	3
TAGCACC	35	0.002169813	16.284575	4
TCCAACG	135	0.0	14.073089	18
CCAACGA	80	2.0000462E-6	13.059849	19
TAGGACC	605	0.0	12.718118	4
CTAGGAC	185	0.0	12.321005	3
TTAGACT	70	1.0901691E-4	12.213431	4
GTATCAA	825	0.0	11.969348	1
GTCTATA	65	8.051053E-4	11.68605	1
GTCCTAA	595	0.0	11.330068	1
GTCCTAC	1195	0.0	11.203206	1
GGGTAAA	85	5.343224E-5	11.170489	1
TAGAAAT	250	0.0	11.019229	4
GGCGAGG	510	0.0	10.987966	19
AGGACCT	850	0.0	10.952904	5
TGTAGGA	1275	0.0	10.874779	2
GTATAGG	70	0.0014965209	10.851333	1
GTTATGA	70	0.0014965209	10.851333	1
CTGTAGG	1230	0.0	10.807221	1
AAATGTC	255	0.0	10.805321	7
>>END_MODULE