FastQCFastQC Report
Thu 2 Feb 2017
SRR4062173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753139
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA7980.10595653657558565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.10383209473948368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG250.002352684335.198629
GTCCGGT300.00573544329.3419231
TACCGTC602.9438888E-729.3321827
CGATGCG407.028539E-427.50074644
TAGGACC8550.024.1861864
GTATCAA9650.022.5765551
TGTAGGA18000.022.1228222
ATACCGT803.613779E-621.9991366
GTAGGAC16950.021.9342423
GTCCTAC15200.021.7168851
TTAGGAC8200.021.194293
TCCTACA16450.020.9975322
GGACCTG11150.020.9139796
CTGTAGG18450.020.6347681
GACGTGA7600.020.5518267
CCTACAG16400.020.5235863
AGGACCT11850.020.4211395
CGTCGTA654.9310934E-420.30689610
GTCCTAT1201.4244506E-820.1725711
AGACCGC550.00448308819.9992166
Produced by FastQC (version 0.11.5)