##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062173_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	753139
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23485306165263	32.0	32.0	32.0	32.0	32.0
2	31.4854628428484	32.0	32.0	32.0	32.0	32.0
3	31.604119558275432	32.0	32.0	32.0	32.0	32.0
4	31.680940702845025	32.0	32.0	32.0	32.0	32.0
5	31.670592015550913	32.0	32.0	32.0	32.0	32.0
6	35.323136897704146	36.0	36.0	36.0	36.0	36.0
7	35.337073236148974	36.0	36.0	36.0	36.0	36.0
8	35.2936483172429	36.0	36.0	36.0	36.0	36.0
9	35.36088291802709	36.0	36.0	36.0	36.0	36.0
10	35.26051100792815	36.0	36.0	36.0	36.0	36.0
11	35.35967596950895	36.0	36.0	36.0	36.0	36.0
12	35.30813037168438	36.0	36.0	36.0	36.0	36.0
13	35.31763193779634	36.0	36.0	36.0	36.0	36.0
14	35.293022934677396	36.0	36.0	36.0	36.0	36.0
15	35.26677412801621	36.0	36.0	36.0	36.0	36.0
16	35.28431272314938	36.0	36.0	36.0	36.0	36.0
17	35.27507272893848	36.0	36.0	36.0	36.0	36.0
18	35.26038354141799	36.0	36.0	36.0	36.0	36.0
19	35.23441356774779	36.0	36.0	36.0	36.0	36.0
20	35.218231959837425	36.0	36.0	36.0	36.0	36.0
21	35.204458937858746	36.0	36.0	36.0	36.0	36.0
22	35.16858906523231	36.0	36.0	36.0	36.0	36.0
23	35.15550250352192	36.0	36.0	36.0	36.0	36.0
24	35.13853883546065	36.0	36.0	36.0	36.0	36.0
25	35.10998899273574	36.0	36.0	36.0	36.0	36.0
26	35.083469319740445	36.0	36.0	36.0	36.0	36.0
27	35.03849355829402	36.0	36.0	36.0	36.0	36.0
28	34.99515760038984	36.0	36.0	36.0	36.0	36.0
29	34.96023443215661	36.0	36.0	36.0	36.0	36.0
30	34.912314990991035	36.0	36.0	36.0	36.0	36.0
31	34.91441951618493	36.0	36.0	36.0	36.0	36.0
32	34.8830693935648	36.0	36.0	36.0	36.0	36.0
33	34.88548727392951	36.0	36.0	36.0	36.0	36.0
34	34.86430393327128	36.0	36.0	36.0	36.0	36.0
35	34.84719553760992	36.0	36.0	36.0	36.0	36.0
36	34.7864696955011	36.0	36.0	36.0	32.0	36.0
37	34.7121553922981	36.0	36.0	36.0	32.0	36.0
38	34.694510575073124	36.0	36.0	36.0	32.0	36.0
39	34.56340463048654	36.0	36.0	36.0	32.0	36.0
40	34.609661695915364	36.0	36.0	36.0	32.0	36.0
41	34.594556914460675	36.0	36.0	36.0	32.0	36.0
42	34.575455526801825	36.0	36.0	36.0	32.0	36.0
43	34.58172794132292	36.0	36.0	36.0	32.0	36.0
44	34.489350571408465	36.0	36.0	36.0	32.0	36.0
45	34.46072504544314	36.0	36.0	36.0	32.0	36.0
46	34.34108312011461	36.0	36.0	36.0	32.0	36.0
47	34.33797081282472	36.0	36.0	36.0	32.0	36.0
48	34.30684375659739	36.0	36.0	36.0	32.0	36.0
49	34.248137462009005	36.0	36.0	36.0	32.0	36.0
50	33.90256645851563	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	6.0
21	22.0
22	48.0
23	131.0
24	390.0
25	865.0
26	1954.0
27	3944.0
28	6702.0
29	10635.0
30	15510.0
31	22286.0
32	32905.0
33	55595.0
34	123191.0
35	478951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16802278166947	18.32335838360655	11.792621990580248	26.715996844143735
2	14.393080316240384	20.451256011833784	41.22624147189343	23.9294222000324
3	19.032020777123922	24.63817955853923	29.00339380935183	27.326405854985026
4	11.868194317383644	16.693996725704018	37.75531475597466	33.682494200937676
5	12.755281561570971	38.34073125943551	35.19297234640618	13.711014832587345
6	32.577781436553295	36.927195088270906	17.60213294809968	12.892890527076121
7	28.61477097853119	30.749835023813667	21.72719776827385	18.908196229381296
8	25.19760628516117	37.611118266349244	19.76408073410088	17.427194714388712
9	26.801825426647667	14.538219372519547	19.200705314689586	39.45924988614319
10	14.975323280297529	28.342444090665868	33.97181662349181	22.710416005544793
11	35.72501224873496	20.76734839120003	25.207697383882653	18.299941976182353
12	24.90363664609056	23.299417504604065	31.68551887500183	20.111426974303548
13	30.373676041208856	21.221447833666826	25.249920665375182	23.15495545974913
14	21.313462720692993	21.646203423272464	26.717511641277376	30.322822214757174
15	22.925515741450116	31.716190504010548	22.550950090222386	22.807343664316946
16	22.51330103287981	26.656903060133814	28.087452880418834	22.742343026567543
17	21.82160265236563	27.995230628077948	27.681078791564374	22.502087927992044
18	21.826706163686023	26.369173204875075	30.525389139027826	21.278731492411076
19	24.92302217784499	25.063500894257235	26.580219587619286	23.433257340278487
20	24.761331920577636	26.444688755579985	27.43467996569022	21.35929935815216
21	23.70120256685685	25.369818851500188	26.155198442784133	24.773780138858832
22	24.132597037200306	27.72303651782739	26.31785102086069	21.826515424111616
23	22.267071549873265	27.71055542204029	27.402378578190746	22.619994449895703
24	24.279872000849785	25.96988567710754	27.80821372140268	21.942028600639997
25	23.312195108997287	26.79495656838455	27.819735559751336	22.07311276286683
26	21.526817901173096	27.795016829652052	28.451738400154024	22.226426869020827
27	22.231575704786387	27.548809245607696	27.544958679441695	22.67465637016422
28	21.961190985813758	27.768070065487223	26.543570514284497	23.72716843441452
29	22.866968518051333	26.512023156692738	26.30037310955612	24.320635215699813
30	22.224582769568237	26.773254231391768	27.37900018722091	23.623162811819086
31	24.283790889246507	25.704922908600352	27.021690752749848	22.989595449403293
32	22.686153368148084	27.467273202864316	27.222295250879995	22.624278178107602
33	21.99852085344366	27.610779977613635	28.34982214391273	22.040877025029975
34	22.59962397030003	27.440342463206978	28.08139874550544	21.878634820987557
35	22.166189994396778	27.769943888105498	28.01438780143878	22.04947831605894
36	21.89681293044957	27.297025640753276	28.417273351329175	22.38888807746798
37	22.3857330835304	27.000625392530075	26.62114092311124	23.99250060082828
38	22.828189749833697	28.30061914201761	26.49471080371618	22.376480304432516
39	23.898068333306778	26.364568229739277	26.912413760137667	22.82494967681628
40	24.58101968032419	26.266368182192373	27.436007743600776	21.716604393882662
41	24.178295911728387	25.726103063216176	28.101257419037886	21.99434360601755
42	23.080641027854092	28.05552494147547	27.328532750500262	21.535301280170177
43	21.838186086531618	27.9803004068452	28.017081124719827	22.164432381903357
44	21.833534058422288	27.97532342853501	27.164278989726505	23.026863523316198
45	22.0622953518819	28.106867147686565	27.205685134679374	22.625152365752164
46	22.588136567696292	26.345965654921322	27.260161381769993	23.805736395612392
47	24.091989430775563	26.588503976737083	26.82126591690678	22.49824067558058
48	22.32475999521982	27.117286983309214	26.60926026742441	23.948692754046554
49	23.5424556110878	26.644368198258967	26.639189713138478	23.173986477514752
50	22.49635189628365	28.765248011302152	25.926299674293052	22.81210041812115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	358.0
1	209.5
2	61.0
3	205.5
4	350.0
5	312.0
6	274.0
7	217.0
8	160.0
9	226.0
10	292.0
11	423.0
12	554.0
13	986.5
14	1419.0
15	1833.5
16	2248.0
17	2254.5
18	2261.0
19	2227.5
20	2194.0
21	2594.5
22	2995.0
23	3597.5
24	4200.0
25	5484.5
26	6769.0
27	9574.5
28	12380.0
29	15607.5
30	18835.0
31	22768.5
32	26702.0
33	32578.0
34	38454.0
35	43824.5
36	49195.0
37	49239.0
38	49283.0
39	47247.5
40	45212.0
41	43572.0
42	41932.0
43	41962.0
44	41992.0
45	43129.0
46	44266.0
47	45283.5
48	46301.0
49	46793.0
50	47285.0
51	46733.5
52	46182.0
53	45220.5
54	44259.0
55	42834.0
56	41409.0
57	38770.5
58	36132.0
59	32965.0
60	29798.0
61	26016.0
62	22234.0
63	19064.0
64	15894.0
65	13425.5
66	10957.0
67	9105.5
68	7254.0
69	6007.0
70	4760.0
71	3916.0
72	3072.0
73	2603.0
74	2134.0
75	1695.0
76	1256.0
77	1016.5
78	777.0
79	635.0
80	493.0
81	388.0
82	283.0
83	212.0
84	141.0
85	106.5
86	72.0
87	52.0
88	32.0
89	20.0
90	8.0
91	7.0
92	6.0
93	4.5
94	3.0
95	4.0
96	5.0
97	4.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033592736533362366
2	0.005443882205011292
3	3.98332844269119E-4
4	0.0
5	0.0
6	3.98332844269119E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.65555229512746E-4
17	0.0
18	2.65555229512746E-4
19	0.0
20	1.32777614756373E-4
21	0.0
22	0.0
23	0.0
24	0.001194998532807357
25	1.32777614756373E-4
26	5.31110459025492E-4
27	3.98332844269119E-4
28	0.001991664221345595
29	0.001194998532807357
30	0.002389997065614714
31	9.29443303294611E-4
32	0.00132777614756373
33	2.65555229512746E-4
34	9.29443303294611E-4
35	1.32777614756373E-4
36	2.65555229512746E-4
37	0.001593331377076476
38	0.0
39	9.29443303294611E-4
40	1.32777614756373E-4
41	0.001194998532807357
42	0.004779994131229428
43	0.0035849955984220707
44	0.004779994131229428
45	0.002257219450858341
46	0.002389997065614714
47	0.001194998532807357
48	0.0038505508279348165
49	0.003053885139396579
50	0.001062220918050984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	753139.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.76451322163905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.22986321321595	52.33444086817347
2	14.555877403703748	19.72743893551679
3	4.384780414124531	8.913975310407771
4	1.6970346857024996	4.599949175874684
5	0.790060371071618	2.6769028230687852
6	0.4017898138859754	1.6336254693237642
7	0.23796190903822692	1.128776105188718
8	0.1546005792579161	0.8381146397756889
9	0.10886507487452099	0.6639469925138283
>10	0.40625648548279	4.643270982412125
>50	0.021283551046194744	0.9764606045027926
>100	0.010641202109202564	1.382501044487897
>500	9.852964868346005E-4	0.48059704875361337
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	798	0.10595653657558565	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	782	0.10383209473948368	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.32777614756373E-4
11	0.0	0.0	0.0	0.0	1.32777614756373E-4
12	0.0	0.0	0.0	0.0	1.32777614756373E-4
13	0.0	0.0	0.0	0.0	1.32777614756373E-4
14	0.0	0.0	0.0	0.0	3.9833284426911897E-4
15	0.0	0.0	0.0	0.0	3.9833284426911897E-4
16	0.0	0.0	0.0	0.0	5.31110459025492E-4
17	0.0	0.0	0.0	1.32777614756373E-4	5.31110459025492E-4
18	0.0	0.0	0.0	1.32777614756373E-4	5.31110459025492E-4
19	0.0	0.0	0.0	1.32777614756373E-4	7.966656885382379E-4
20	0.0	0.0	0.0	3.9833284426911897E-4	7.966656885382379E-4
21	0.0	0.0	0.0	5.31110459025492E-4	0.001062220918050984
22	0.0	0.0	0.0	7.966656885382379E-4	0.00132777614756373
23	0.0	0.0	0.0	0.001194998532807357	0.00132777614756373
24	0.0	0.0	0.0	0.001858886606589222	0.00132777614756373
25	0.0	0.0	0.0	0.002522774680371087	0.00132777614756373
26	0.0	0.0	0.0	0.0035849955984220707	0.00132777614756373
27	0.0	0.0	0.0	0.004912771745985801	0.00132777614756373
28	0.0	0.0	0.0	0.006904435967331396	0.00132777614756373
29	0.0	0.0	0.0	0.008763322573920618	0.00132777614756373
30	0.0	0.0	0.0	0.013676094319906419	0.00132777614756373
31	0.0	0.0	0.0	0.02124441836101968	0.00132777614756373
32	0.0	0.0	0.0	0.027219411025056463	0.00132777614756373
33	0.0	0.0	0.0	0.03372551414811874	0.001460553762320103
34	0.0	0.0	0.0	0.04222328149252661	0.001460553762320103
35	0.0	0.0	0.0	0.05098660406644723	0.001460553762320103
36	0.0	0.0	0.0	0.05988270425512422	0.001460553762320103
37	0.0	0.0	0.0	0.07674546132918358	0.001460553762320103
38	0.0	0.0	0.0	0.10104376482959984	0.001460553762320103
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	25	0.0023526843	35.19862	9
GTCCGGT	30	0.005735443	29.341923	1
TACCGTC	60	2.9438888E-7	29.332182	7
CGATGCG	40	7.028539E-4	27.500746	44
TAGGACC	855	0.0	24.186186	4
GTATCAA	965	0.0	22.576555	1
TGTAGGA	1800	0.0	22.122822	2
ATACCGT	80	3.613779E-6	21.999136	6
GTAGGAC	1695	0.0	21.934242	3
GTCCTAC	1520	0.0	21.716885	1
TTAGGAC	820	0.0	21.19429	3
TCCTACA	1645	0.0	20.997532	2
GGACCTG	1115	0.0	20.913979	6
CTGTAGG	1845	0.0	20.634768	1
GACGTGA	760	0.0	20.551826	7
CCTACAG	1640	0.0	20.523586	3
AGGACCT	1185	0.0	20.421139	5
CGTCGTA	65	4.9310934E-4	20.306896	10
GTCCTAT	120	1.4244506E-8	20.172571	1
AGACCGC	55	0.004483088	19.999216	6
>>END_MODULE