FastQCFastQC Report
Thu 2 Feb 2017
SRR4062172_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062172_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80.4398020890599231No Hit
ACCATAAACGATGCCGACTGGCGAT50.2748763056624519No Hit
GTTATTGCTCAATCTCGGGTGGCTG40.21990104452996154No Hit
TATCAACGCAGAGTACTTTTTTTTT40.21990104452996154No Hit
GGTATCAACGCAGAGTACTTTTTTT40.21990104452996154No Hit
AGTGGAGCCTGCGGCTTAATTTGAC30.16492578339747113No Hit
GTGGTGCATGGCCGTTCTTAGTTGG30.16492578339747113No Hit
GCCCACATAGCATGCAAGTTTGCTG30.16492578339747113No Hit
GGGTAGGCACACGCTGAGCCAGTCA30.16492578339747113No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC30.16492578339747113No Hit
CTTTATTGCTTCGGGCGTGTGGGGC20.10995052226498077No Hit
GTATCTGATCGTCTTCGAACCTCCG20.10995052226498077No Hit
CTGCAGGAAATGGAGATGCTGAACG20.10995052226498077No Hit
GTATCAACGCAGAGTACATGGGCTC20.10995052226498077No Hit
ATATATGTGTGTGCACCACATGTGT20.10995052226498077No Hit
AGATAACCTCGGGCCGATCGCACGC20.10995052226498077No Hit
CTCCCAGACTGTCCCTTTCAAAGGT20.10995052226498077No Hit
AAACTGCACAGTCTTAACAGCATCC20.10995052226498077No Hit
CTACCATGGTGACCACGGGTGACGG20.10995052226498077No Hit
TCAGTGGACTCTTTATTGCTTCGGG20.10995052226498077No Hit
TCTTGGACCGGCGCAAGACGGACCA20.10995052226498077No Hit
TATCAACGCAGAGTACATGGGGGGT20.10995052226498077No Hit
CCCATGACCCGCCGGGCAGCTTCCG20.10995052226498077No Hit
ATCAGATACCGTCGTAGTTCCGACC20.10995052226498077No Hit
GCCATCACCCCCACGGCTGGAGACT20.10995052226498077No Hit
GTACGTAGCCATCCAGGCTGTGCTG20.10995052226498077No Hit
GCATATAGCCTAGTCCATGCTCTCG20.10995052226498077No Hit
CAATACGAATGCCCCCGGCCGTCCC20.10995052226498077No Hit
ACCATACTCCCCCCGGAACCCAAAG20.10995052226498077No Hit
GCTCCGGCCGGGGGTCGGGCGCCGG20.10995052226498077No Hit
GATCGAGAGTGACCGATTAGCGCTG20.10995052226498077No Hit
GTATCAACGCAGAGTACATGGGGAA20.10995052226498077No Hit
ACTCAGACCGGTGCCCCATTTAGCT20.10995052226498077No Hit
GGCTACTGCTGCATCATCTTCCTCC20.10995052226498077No Hit
TCTAGGCACTTGGTTAACTTTTCCT20.10995052226498077No Hit
CATCATCTCCCACCCAGCTTTTTCC20.10995052226498077No Hit
GCCTACCATGGTGACCACGGGTGAC20.10995052226498077No Hit
TTGTCACACTGTGCTGACAGGAGAC20.10995052226498077No Hit
AGACACACCCGCAGTGGTATCCTGC20.10995052226498077No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20.10995052226498077No Hit
TTATTGATCAGCTGGATCTTGTCTG20.10995052226498077No Hit
GTTGTTGAACAAACAGTAGCGATTC20.10995052226498077No Hit
TTGCAGGGCTGAGGCAGTGTGGAAG20.10995052226498077No Hit
CCCCTTGCCTCTCGGCGCCCCCTCG20.10995052226498077No Hit
TCCATATTCTCCTGGCGCAGCTTGG20.10995052226498077No Hit
CCTCGAAAGAGTCCTGTATTGTTAT20.10995052226498077No Hit
GAACAGCTCTGTCCGCTTCTTGAAT20.10995052226498077No Hit
ATATAAGGCTTTGAAGACTTCATAA20.10995052226498077No Hit
CTCCTGAGCTACAGAAGGAATGGTT20.10995052226498077No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20.10995052226498077No Hit
GTTGTCGGCTGCGCCGGCGGCCAAA20.10995052226498077No Hit
GTACTGGGAACGCCGCAGGTGAGAG20.10995052226498077No Hit
GTATCAACGCAGAGTACATGGGAAT20.10995052226498077No Hit
ACAATACAGGACTCTTTCGAGGCCC20.10995052226498077No Hit
ATGTCATAGCGTGTCGTTCGGCGGC20.10995052226498077No Hit
GGACAGGATTGACAGATTGATAGCT20.10995052226498077No Hit
GTCCTGCACCGTCACTAGCCATTCC20.10995052226498077No Hit
GCCCAGCTTCTCCCCGCTCTTGGAC20.10995052226498077No Hit
GGCTACGCCAGTACGCAGAGAAGAA20.10995052226498077No Hit
CAGCTGCTCCAGACCAGGGAAATGG20.10995052226498077No Hit
GTCCTATTCCATTATTCCTAGCTGC20.10995052226498077No Hit
CTGTCCACCTTCCAGCAGATGTGGA20.10995052226498077No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)