##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062172_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1819
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1412864211105	32.0	32.0	32.0	32.0	32.0
2	30.63111599780099	32.0	32.0	32.0	32.0	32.0
3	30.55140186915888	32.0	32.0	32.0	32.0	32.0
4	30.74546454095657	32.0	32.0	32.0	32.0	32.0
5	30.578889499725122	32.0	32.0	32.0	32.0	32.0
6	34.229246838922485	36.0	36.0	36.0	32.0	36.0
7	34.277075316107755	36.0	36.0	36.0	32.0	36.0
8	34.384826827927434	36.0	36.0	36.0	32.0	36.0
9	34.391423859263334	36.0	36.0	36.0	32.0	36.0
10	33.88894997251237	36.0	36.0	36.0	32.0	36.0
11	34.30291368884002	36.0	36.0	36.0	32.0	36.0
12	34.105002748763056	36.0	36.0	36.0	32.0	36.0
13	34.12424409015943	36.0	36.0	36.0	32.0	36.0
14	34.07916437603079	36.0	36.0	36.0	32.0	36.0
15	34.032985156679494	36.0	36.0	36.0	32.0	36.0
16	33.91313908741066	36.0	36.0	36.0	32.0	36.0
17	33.99395272127543	36.0	36.0	36.0	32.0	36.0
18	33.74546454095657	36.0	36.0	36.0	32.0	36.0
19	33.8609125893348	36.0	36.0	36.0	32.0	36.0
20	33.87300714678395	36.0	36.0	36.0	32.0	36.0
21	33.82792743265531	36.0	36.0	36.0	32.0	36.0
22	33.74766355140187	36.0	36.0	36.0	27.0	36.0
23	33.97910940076965	36.0	36.0	36.0	32.0	36.0
24	33.65530511269929	36.0	36.0	36.0	32.0	36.0
25	33.573391973611876	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	1.0
18	0.0
19	0.0
20	3.0
21	3.0
22	5.0
23	11.0
24	5.0
25	16.0
26	22.0
27	29.0
28	51.0
29	67.0
30	67.0
31	103.0
32	130.0
33	177.0
34	384.0
35	742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.06926882902694	18.58163826278175	11.76470588235294	23.58438702583837
2	18.63661352391424	18.471687740516767	34.96426608026388	27.92743265530511
3	19.406267179769106	22.539857064321055	27.54260582737768	30.51126992853216
4	14.183617372182516	15.063221550302364	34.139637163276525	36.61352391423859
5	17.37218251786696	33.80978559648158	31.94062671797691	16.877405167674546
6	36.08360836083608	31.62816281628163	16.666666666666664	15.62156215621562
7	31.167400881057265	31.277533039647576	17.566079295154186	19.98898678414097
8	29.49917446340121	31.31535498073748	18.436984039625756	20.748486516235552
9	25.055066079295152	15.638766519823788	18.55726872246696	40.7488986784141
10	17.63085399449036	25.6198347107438	29.80716253443526	26.942148760330582
11	39.46064942212438	19.592735277930657	20.36323610346725	20.58337919647771
12	25.66079295154185	22.136563876651984	27.75330396475771	24.44933920704846
13	30.198019801980198	19.251925192519252	24.147414741474147	26.4026402640264
14	23.762376237623762	19.746974697469746	23.707370737073706	32.78327832783278
15	27.242707760044027	24.986241056686847	22.619702806824435	25.151348376444687
16	27.007700770077008	25.577557755775576	20.462046204620464	26.952695269526956
17	26.08695652173913	25.536598789212988	21.574023115024765	26.802421574023118
18	26.292629262926294	25.90759075907591	24.257425742574256	23.54235423542354
19	28.16281628162816	24.257425742574256	24.09240924092409	23.487348734873486
20	27.997799779978	23.817381738173818	24.367436743674368	23.817381738173818
21	29.003852504127682	22.894881673087507	25.09631260319207	23.004953219592736
22	27.793065492570168	22.729774353329667	24.76609796367639	24.711062190423775
23	25.027502750275026	22.827282728272827	23.762376237623762	28.38283828382838
24	25.13751375137514	26.237623762376238	22.33223322332233	26.292629262926294
25	27.722772277227726	22.937293729372936	23.26732673267327	26.072607260726073
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	3.0
9	3.0
10	3.5
11	4.0
12	4.0
13	4.0
14	2.5
15	1.0
16	1.0
17	1.0
18	4.0
19	7.0
20	7.0
21	7.0
22	7.5
23	8.0
24	8.0
25	8.0
26	18.5
27	29.0
28	29.0
29	29.0
30	38.5
31	48.0
32	48.0
33	48.0
34	68.5
35	89.0
36	89.0
37	89.0
38	113.5
39	138.0
40	138.0
41	138.0
42	156.0
43	174.0
44	174.0
45	174.0
46	203.0
47	232.0
48	232.0
49	232.0
50	251.5
51	271.0
52	271.0
53	271.0
54	248.0
55	225.0
56	225.0
57	225.0
58	223.5
59	222.0
60	222.0
61	222.0
62	191.5
63	161.0
64	161.0
65	161.0
66	133.5
67	106.0
68	106.0
69	106.0
70	79.5
71	53.0
72	53.0
73	53.0
74	37.0
75	21.0
76	21.0
77	21.0
78	17.0
79	13.0
80	13.0
81	13.0
82	8.0
83	3.0
84	3.0
85	3.0
86	4.5
87	6.0
88	6.0
89	6.0
90	5.0
91	4.0
92	4.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.054975261132490384
7	0.16492578339747113
8	0.10995052226498077
9	0.16492578339747113
10	0.21990104452996154
11	0.10995052226498077
12	0.16492578339747113
13	0.054975261132490384
14	0.054975261132490384
15	0.10995052226498077
16	0.054975261132490384
17	0.10995052226498077
18	0.054975261132490384
19	0.054975261132490384
20	0.054975261132490384
21	0.10995052226498077
22	0.10995052226498077
23	0.054975261132490384
24	0.054975261132490384
25	0.054975261132490384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1819.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.49202858713579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.43062751871042	92.08356239692138
2	2.993667242371906	5.7174271577789995
3	0.28785261945883706	0.8246289169873556
4	0.17271157167530224	0.6597031335898845
5	0.05757052389176742	0.2748763056624519
6	0.0	0.0
7	0.0	0.0
8	0.05757052389176742	0.4398020890599231
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8	0.4398020890599231	No Hit
ACCATAAACGATGCCGACTGGCGAT	5	0.2748763056624519	No Hit
GTTATTGCTCAATCTCGGGTGGCTG	4	0.21990104452996154	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4	0.21990104452996154	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4	0.21990104452996154	No Hit
AGTGGAGCCTGCGGCTTAATTTGAC	3	0.16492578339747113	No Hit
GTGGTGCATGGCCGTTCTTAGTTGG	3	0.16492578339747113	No Hit
GCCCACATAGCATGCAAGTTTGCTG	3	0.16492578339747113	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	3	0.16492578339747113	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	3	0.16492578339747113	No Hit
CTTTATTGCTTCGGGCGTGTGGGGC	2	0.10995052226498077	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2	0.10995052226498077	No Hit
CTGCAGGAAATGGAGATGCTGAACG	2	0.10995052226498077	No Hit
GTATCAACGCAGAGTACATGGGCTC	2	0.10995052226498077	No Hit
ATATATGTGTGTGCACCACATGTGT	2	0.10995052226498077	No Hit
AGATAACCTCGGGCCGATCGCACGC	2	0.10995052226498077	No Hit
CTCCCAGACTGTCCCTTTCAAAGGT	2	0.10995052226498077	No Hit
AAACTGCACAGTCTTAACAGCATCC	2	0.10995052226498077	No Hit
CTACCATGGTGACCACGGGTGACGG	2	0.10995052226498077	No Hit
TCAGTGGACTCTTTATTGCTTCGGG	2	0.10995052226498077	No Hit
TCTTGGACCGGCGCAAGACGGACCA	2	0.10995052226498077	No Hit
TATCAACGCAGAGTACATGGGGGGT	2	0.10995052226498077	No Hit
CCCATGACCCGCCGGGCAGCTTCCG	2	0.10995052226498077	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2	0.10995052226498077	No Hit
GCCATCACCCCCACGGCTGGAGACT	2	0.10995052226498077	No Hit
GTACGTAGCCATCCAGGCTGTGCTG	2	0.10995052226498077	No Hit
GCATATAGCCTAGTCCATGCTCTCG	2	0.10995052226498077	No Hit
CAATACGAATGCCCCCGGCCGTCCC	2	0.10995052226498077	No Hit
ACCATACTCCCCCCGGAACCCAAAG	2	0.10995052226498077	No Hit
GCTCCGGCCGGGGGTCGGGCGCCGG	2	0.10995052226498077	No Hit
GATCGAGAGTGACCGATTAGCGCTG	2	0.10995052226498077	No Hit
GTATCAACGCAGAGTACATGGGGAA	2	0.10995052226498077	No Hit
ACTCAGACCGGTGCCCCATTTAGCT	2	0.10995052226498077	No Hit
GGCTACTGCTGCATCATCTTCCTCC	2	0.10995052226498077	No Hit
TCTAGGCACTTGGTTAACTTTTCCT	2	0.10995052226498077	No Hit
CATCATCTCCCACCCAGCTTTTTCC	2	0.10995052226498077	No Hit
GCCTACCATGGTGACCACGGGTGAC	2	0.10995052226498077	No Hit
TTGTCACACTGTGCTGACAGGAGAC	2	0.10995052226498077	No Hit
AGACACACCCGCAGTGGTATCCTGC	2	0.10995052226498077	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2	0.10995052226498077	No Hit
TTATTGATCAGCTGGATCTTGTCTG	2	0.10995052226498077	No Hit
GTTGTTGAACAAACAGTAGCGATTC	2	0.10995052226498077	No Hit
TTGCAGGGCTGAGGCAGTGTGGAAG	2	0.10995052226498077	No Hit
CCCCTTGCCTCTCGGCGCCCCCTCG	2	0.10995052226498077	No Hit
TCCATATTCTCCTGGCGCAGCTTGG	2	0.10995052226498077	No Hit
CCTCGAAAGAGTCCTGTATTGTTAT	2	0.10995052226498077	No Hit
GAACAGCTCTGTCCGCTTCTTGAAT	2	0.10995052226498077	No Hit
ATATAAGGCTTTGAAGACTTCATAA	2	0.10995052226498077	No Hit
CTCCTGAGCTACAGAAGGAATGGTT	2	0.10995052226498077	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2	0.10995052226498077	No Hit
GTTGTCGGCTGCGCCGGCGGCCAAA	2	0.10995052226498077	No Hit
GTACTGGGAACGCCGCAGGTGAGAG	2	0.10995052226498077	No Hit
GTATCAACGCAGAGTACATGGGAAT	2	0.10995052226498077	No Hit
ACAATACAGGACTCTTTCGAGGCCC	2	0.10995052226498077	No Hit
ATGTCATAGCGTGTCGTTCGGCGGC	2	0.10995052226498077	No Hit
GGACAGGATTGACAGATTGATAGCT	2	0.10995052226498077	No Hit
GTCCTGCACCGTCACTAGCCATTCC	2	0.10995052226498077	No Hit
GCCCAGCTTCTCCCCGCTCTTGGAC	2	0.10995052226498077	No Hit
GGCTACGCCAGTACGCAGAGAAGAA	2	0.10995052226498077	No Hit
CAGCTGCTCCAGACCAGGGAAATGG	2	0.10995052226498077	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2	0.10995052226498077	No Hit
CTGTCCACCTTCCAGCAGATGTGGA	2	0.10995052226498077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE