##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1819
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.007696536558548	32.0	32.0	32.0	32.0	32.0
2	31.22429906542056	32.0	32.0	32.0	32.0	32.0
3	31.468389224848817	32.0	32.0	32.0	32.0	32.0
4	31.540956569543706	32.0	32.0	32.0	32.0	32.0
5	31.544255085211656	32.0	32.0	32.0	32.0	32.0
6	35.066520065970316	36.0	36.0	36.0	36.0	36.0
7	35.15503023639362	36.0	36.0	36.0	36.0	36.0
8	35.18966465090709	36.0	36.0	36.0	36.0	36.0
9	35.21990104452996	36.0	36.0	36.0	36.0	36.0
10	35.09070918086861	36.0	36.0	36.0	36.0	36.0
11	35.09180868609126	36.0	36.0	36.0	36.0	36.0
12	35.21440351841671	36.0	36.0	36.0	36.0	36.0
13	35.14238592633315	36.0	36.0	36.0	36.0	36.0
14	35.091258933479935	36.0	36.0	36.0	36.0	36.0
15	35.150082462891696	36.0	36.0	36.0	36.0	36.0
16	35.135788894997255	36.0	36.0	36.0	36.0	36.0
17	35.13413963716328	36.0	36.0	36.0	36.0	36.0
18	35.322704782847715	36.0	36.0	36.0	36.0	36.0
19	35.11874656404618	36.0	36.0	36.0	36.0	36.0
20	35.13798790544255	36.0	36.0	36.0	36.0	36.0
21	35.1588785046729	36.0	36.0	36.0	36.0	36.0
22	35.156679494227596	36.0	36.0	36.0	36.0	36.0
23	35.05937328202309	36.0	36.0	36.0	36.0	36.0
24	34.9758108851017	36.0	36.0	36.0	32.0	36.0
25	35.032985156679494	36.0	36.0	36.0	32.0	36.0
26	34.94282572842221	36.0	36.0	36.0	32.0	36.0
27	34.92798240791644	36.0	36.0	36.0	36.0	36.0
28	34.92468389224849	36.0	36.0	36.0	32.0	36.0
29	34.90434304562947	36.0	36.0	36.0	32.0	36.0
30	34.866959868059375	36.0	36.0	36.0	32.0	36.0
31	34.835623969213856	36.0	36.0	36.0	32.0	36.0
32	34.91094007696537	36.0	36.0	36.0	32.0	36.0
33	34.842221000549756	36.0	36.0	36.0	32.0	36.0
34	34.815832875206155	36.0	36.0	36.0	32.0	36.0
35	34.87300714678395	36.0	36.0	36.0	36.0	36.0
36	34.78614623419461	36.0	36.0	36.0	32.0	36.0
37	34.922484881803186	36.0	36.0	36.0	32.0	36.0
38	34.76305662451897	36.0	36.0	36.0	32.0	36.0
39	34.88235294117647	36.0	36.0	36.0	32.0	36.0
40	34.76195711929632	36.0	36.0	36.0	32.0	36.0
41	34.719076415612975	36.0	36.0	36.0	32.0	36.0
42	34.51291918636613	36.0	36.0	36.0	32.0	36.0
43	34.73117097306212	36.0	36.0	36.0	32.0	36.0
44	34.76635514018692	36.0	36.0	36.0	32.0	36.0
45	34.677295217152285	36.0	36.0	36.0	32.0	36.0
46	34.59868059373282	36.0	36.0	36.0	32.0	36.0
47	34.570643210555254	36.0	36.0	36.0	32.0	36.0
48	34.41341396371633	36.0	36.0	36.0	32.0	36.0
49	34.415612974161625	36.0	36.0	36.0	32.0	36.0
50	34.12699285321605	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	7.0
27	10.0
28	12.0
29	33.0
30	38.0
31	48.0
32	84.0
33	149.0
34	316.0
35	1121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.71947194719472	16.5016501650165	13.366336633663368	25.412541254125415
2	16.987355689939527	18.471687740516767	35.67894447498626	28.86201209455745
3	17.811984606926885	23.254535459043428	29.136888400219902	29.796591533809785
4	16.052776250687188	14.183617372182516	32.71028037383177	37.053326003298515
5	16.492578339747112	33.974711379879054	30.34634414513469	19.18636613523914
6	35.843870258383724	34.41451346893898	15.557998900494777	14.183617372182516
7	32.105552501374376	28.36723474436503	18.58163826278175	20.945574491478837
8	28.092358438702586	31.006047278724573	19.29631665750412	21.60527762506872
9	27.32270478284772	15.503023639362286	17.26223199560198	39.912039582188015
10	17.647058823529413	26.717976910390323	28.916987355689937	26.717976910390323
11	37.65805387575591	19.736118746564046	20.39582188015393	22.210005497526115
12	25.89334799340297	22.869708631115998	27.872457394172624	23.364485981308412
13	31.335898845519516	18.031885651456843	23.694337548103352	26.937877954920285
14	23.914238592633318	18.91148982957669	23.309510720175922	33.86476085761407
15	23.859263331500824	28.147333699835077	21.330401319406267	26.663001649257833
16	26.38812534359538	25.673446948873007	23.08960967564596	24.848818031885653
17	25.728422210005498	25.233644859813083	22.704782847718526	26.333150082462893
18	25.233644859813083	26.8829026937878	25.89334799340297	21.99010445299615
19	26.333150082462893	24.903793293018143	24.024189114898295	24.738867509620672
20	29.081913139087412	22.374931280923587	24.244090159428257	24.299065420560748
21	27.212754260582738	25.06871907641561	24.354040681693238	23.364485981308412
22	26.553051126992855	23.804288070368333	24.024189114898295	25.618471687740517
23	24.518966465090706	23.639362286970865	25.178669598680592	26.663001649257833
24	26.333150082462893	22.594832325453545	25.398570643210554	25.673446948873007
25	25.56349642660803	24.95876855415063	25.838372732270475	23.639362286970865
26	24.079164376030786	24.79384277075316	26.00329851566795	25.123694337548102
27	25.178669598680592	24.134139637163276	24.95876855415063	25.728422210005498
28	26.333150082462893	22.92468389224849	24.848818031885653	25.89334799340297
29	25.398570643210554	21.49532710280374	26.498075865860365	26.608026388125346
30	24.299065420560748	25.89334799340297	25.123694337548102	24.683892248488178
31	26.663001649257833	23.859263331500824	23.254535459043428	26.223199560197912
32	27.102803738317753	22.539857064321055	24.518966465090706	25.838372732270475
33	25.89334799340297	23.859263331500824	24.46399120395822	25.783397471137988
34	26.38812534359538	22.97965915338098	24.738867509620672	25.89334799340297
35	25.508521165475535	22.814733369983507	25.56349642660803	26.113249037932928
36	25.89334799340297	23.58438702583837	25.178669598680592	25.343595382078064
37	26.8829026937878	23.309510720175922	22.704782847718526	27.102803738317753
38	23.58438702583837	25.508521165475535	25.618471687740517	25.288620120945577
39	27.59758108851017	22.97965915338098	23.914238592633318	25.508521165475535
40	26.717976910390323	21.275426058273776	24.5739417262232	27.432655305112704
41	27.102803738317753	22.210005497526115	25.398570643210554	25.288620120945577
42	27.26772952171523	23.914238592633318	24.299065420560748	24.518966465090706
43	24.903793293018143	23.08960967564596	24.518966465090706	27.48763056624519
44	26.278174821330403	22.319956019791093	24.244090159428257	27.15777899945025
45	26.113249037932928	23.4194612424409	23.08960967564596	27.377680043980206
46	25.89334799340297	22.97965915338098	23.969213853765805	27.15777899945025
47	26.38812534359538	23.694337548103352	24.134139637163276	25.783397471137988
48	25.728422210005498	22.264980758658602	23.08960967564596	28.916987355689937
49	25.453545904343045	22.97965915338098	24.5739417262232	26.992853216052776
50	26.608026388125346	23.914238592633318	23.639362286970865	25.838372732270475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	2.5
14	4.0
15	4.0
16	4.0
17	3.5
18	3.0
19	2.5
20	2.0
21	3.0
22	4.0
23	4.0
24	4.0
25	5.0
26	6.0
27	6.0
28	6.0
29	4.5
30	3.0
31	7.0
32	11.0
33	19.5
34	28.0
35	29.5
36	31.0
37	37.0
38	43.0
39	55.0
40	67.0
41	68.5
42	70.0
43	88.0
44	106.0
45	113.0
46	120.0
47	117.0
48	114.0
49	134.5
50	155.0
51	157.5
52	160.0
53	151.5
54	143.0
55	144.5
56	146.0
57	147.0
58	148.0
59	142.0
60	136.0
61	113.0
62	90.0
63	81.0
64	72.0
65	64.0
66	56.0
67	42.5
68	29.0
69	23.0
70	17.0
71	16.0
72	15.0
73	14.5
74	14.0
75	9.0
76	4.0
77	2.5
78	1.0
79	1.0
80	1.0
81	1.5
82	2.0
83	1.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.054975261132490384
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1819.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.4815832875206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.75213675213675	93.34799340296867
2	2.849002849002849	5.497526113249037
3	0.39886039886039887	1.154480483782298
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3	0.16492578339747113	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3	0.16492578339747113	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA	3	0.16492578339747113	No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTT	3	0.16492578339747113	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.16492578339747113	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3	0.16492578339747113	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG	3	0.16492578339747113	No Hit
GTCTACAGGGTCTCAAAAAAAAAAAAGCAAACAACAAAACTGGAATGTTC	2	0.10995052226498077	No Hit
GAACCGCTCGTTGCCAATAGTGATGACCTGGCCGTCAGGCAGCTCATAGC	2	0.10995052226498077	No Hit
ATTTGACCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG	2	0.10995052226498077	No Hit
CTTCTGGAAACAAGGTTAAAGCTGAGCCGGCCAAAATTGATGCTTTCCGA	2	0.10995052226498077	No Hit
GTCGTCATCATGGTCATCGTTGCTAGTCTGGTTCTCCTGGGAGGTGTGAT	2	0.10995052226498077	No Hit
TGGCTATTCCATGCAAGGGCTCTGCAAATTCGGCTTTTATGAAGTCTTCA	2	0.10995052226498077	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG	2	0.10995052226498077	No Hit
GTACAGGGACTTGCCATATTTCCTCTTGAATTCAGATCTGATTTTCAGCA	2	0.10995052226498077	No Hit
ATGGGAAGCTGCACGTGAACGGTCAAATCACCGTTCATTCTTTGGATGCT	2	0.10995052226498077	No Hit
CTCTGGAAGAGATCAGTCCTGTCCGTCCATAAAGCTGCCTTGTGTGCCCC	2	0.10995052226498077	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT	2	0.10995052226498077	No Hit
CAGATGTTCATGACACTGGCCACAACGGCAGACCCTGCAGAGCCCACGAT	2	0.10995052226498077	No Hit
GCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAG	2	0.10995052226498077	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	2	0.10995052226498077	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	2	0.10995052226498077	No Hit
CTTCATGATGGAGTTGAAAGTGGTCTCATGGATACCACAGGACTCCATGC	2	0.10995052226498077	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC	2	0.10995052226498077	No Hit
GCAGATGCACCCAACAGTGGTGATCAGTGCTTACCGCATGGCACTGGATG	2	0.10995052226498077	No Hit
TCTTAATGGACAAAGCCTTCATAGCAGCGCAGTGGCGGCAACGTACAAGT	2	0.10995052226498077	No Hit
GCGGCTCAGGGCCCGCAAGGAATGCTTTGACCGGGATGGCAAGGAACGGG	2	0.10995052226498077	No Hit
TATTTTACCAGTATCCTCAGTATCTAAGTGCGTATCCCAGTTACAAAGAA	2	0.10995052226498077	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGTCGCTAGAGGTGAAA	2	0.10995052226498077	No Hit
ATCCTGCTTCTTCTCATTTTGGAGAGTGAACTTGGGCAGACATGGGTGCC	2	0.10995052226498077	No Hit
ATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCG	2	0.10995052226498077	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG	2	0.10995052226498077	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2	0.10995052226498077	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	2	0.10995052226498077	No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCA	2	0.10995052226498077	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2	0.10995052226498077	No Hit
GATTAAGACTGGGGTGGCAGGTGTTAGGGGTAATATTCATTTAGCCTTCT	2	0.10995052226498077	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA	2	0.10995052226498077	No Hit
GGAGAGAGGCGGACACTTCACGTAGTCCAGTGTCTTGAGTGTGAGGAGTG	2	0.10995052226498077	No Hit
GTCGTGTTCAGCAGGATGTCTCCTGGGTGCTCTTGATTGCCGCTGTGGAA	2	0.10995052226498077	No Hit
CTCATGAACTGAAGGTCATTGCTCTGAACACCTGTCTCAGCACCTAGCTC	2	0.10995052226498077	No Hit
GATAAGTGGTCCCCAGCACTGCAGATCCGCACAGTTCTGCTATCAATCCA	2	0.10995052226498077	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC	2	0.10995052226498077	No Hit
TATCAACGCAGAGTACATGGGCTCTAGCTGCCATCTTGCGTCCCCGCGTG	2	0.10995052226498077	No Hit
GCATCAGACACATGATCCGAAAGAACAAGTACCGCCCTGATCTGCGTATG	2	0.10995052226498077	No Hit
GTCCCGTCCACGCCCACGGTTACATTCAGGTGGTCTAGGCCTCTGTTCTC	2	0.10995052226498077	No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG	2	0.10995052226498077	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT	2	0.10995052226498077	No Hit
CCTACGTGCTGTGGACTTCGCGGAGCGACACGGCTACATTAAAGGCATCG	2	0.10995052226498077	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.10995052226498077	No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC	2	0.10995052226498077	No Hit
AGGCTGTCCTGGCCCGAAGCAAGCTTGAGAGTCTGTGCCGGGAAGCTGCA	2	0.10995052226498077	No Hit
CGAAGAGAGTCTCCAGCAGGCGCTTGATGTAGTGGAACGAGTGGCACATG	2	0.10995052226498077	No Hit
ATAGCACAGAAGCCCATCATGTCCATCCCAGACTGGTTTCTAGTAGGCTG	2	0.10995052226498077	No Hit
TGTCGACACTCTGCACATGCTCCTCAAATTTGGTGATCTCCTCCTCGAGC	2	0.10995052226498077	No Hit
GCTTAATTTGACCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAG	2	0.10995052226498077	No Hit
CAGTAAGAGTGACCTTGGCCGGAAGATCAAGGAGGGGGTAGAAGAGGCCG	2	0.10995052226498077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.05497526113249038	0.0
32	0.0	0.0	0.0	0.05497526113249038	0.0
33	0.0	0.0	0.0	0.05497526113249038	0.0
34	0.0	0.0	0.0	0.05497526113249038	0.0
35	0.0	0.0	0.0	0.05497526113249038	0.0
36	0.0	0.0	0.0	0.05497526113249038	0.0
37	0.0	0.0	0.0	0.05497526113249038	0.0
38	0.0	0.0	0.0	0.10995052226498075	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE