FastQCFastQC Report
Thu 2 Feb 2017
SRR4062171_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062171_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169810
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39360.18139837128596512No Hit
TATCAACGCAGAGTACTTTTTTTTT23570.10862702264253553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10300.014.8429721
CAAATCG1155.324182E-912.39184613
GTATCAA26650.012.1859981
TTTGCGC1701.0913936E-1111.17463917
TAGAACC1451.2169039E-911.1384174
CGAACGA1201.2776763E-711.08228416
GTATACG600.00589623711.078451
CGGTCCA3300.010.94189310
GGGTCGA700.001489547810.8591216
GTAATAC1053.4725654E-610.8558643
ATTTGCG1605.456968E-1010.68648816
CTAGACA2050.010.6590024
GTCCTAA2500.010.6353111
TTGCGCG1701.4006218E-1010.61639718
CGCCGGT3250.010.5252377
CGAGCCG3250.010.52232315
AAGACGG3850.010.3650465
GTGTTAT1106.079501E-610.359071
GCGCCGA2700.010.20179719
AAATCGC1409.696123E-810.17831214
Produced by FastQC (version 0.11.5)