##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062171_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2169810
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.270191860116785	32.0	32.0	32.0	32.0	32.0
2	30.822427770173427	32.0	32.0	32.0	32.0	32.0
3	30.84290283481042	32.0	32.0	32.0	32.0	32.0
4	30.853078380134665	32.0	32.0	32.0	32.0	32.0
5	30.76001078435439	32.0	32.0	32.0	32.0	32.0
6	34.419334872638615	36.0	36.0	36.0	32.0	36.0
7	34.3536166761145	36.0	36.0	36.0	32.0	36.0
8	34.318137993649216	36.0	36.0	36.0	32.0	36.0
9	34.450306708882344	36.0	36.0	36.0	32.0	36.0
10	34.14057728556878	36.0	36.0	36.0	32.0	36.0
11	34.43040450546361	36.0	36.0	36.0	32.0	36.0
12	34.26289306436969	36.0	36.0	36.0	32.0	36.0
13	34.34039247676064	36.0	36.0	36.0	32.0	36.0
14	34.24941538660067	36.0	36.0	36.0	32.0	36.0
15	34.20188956636756	36.0	36.0	36.0	32.0	36.0
16	34.20468243763279	36.0	36.0	36.0	32.0	36.0
17	34.1360672132583	36.0	36.0	36.0	32.0	36.0
18	34.1468981155032	36.0	36.0	36.0	32.0	36.0
19	34.14539429719653	36.0	36.0	36.0	32.0	36.0
20	34.12733741664017	36.0	36.0	36.0	32.0	36.0
21	34.115834105290325	36.0	36.0	36.0	32.0	36.0
22	34.07650808135274	36.0	36.0	36.0	32.0	36.0
23	34.050589222097784	36.0	36.0	36.0	32.0	36.0
24	34.01879980274771	36.0	36.0	36.0	32.0	36.0
25	33.667969545720595	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	35.0
5	160.0
6	440.0
7	77.0
8	399.0
9	283.0
10	175.0
11	37.0
12	90.0
13	95.0
14	284.0
15	508.0
16	767.0
17	977.0
18	1363.0
19	1905.0
20	2846.0
21	4115.0
22	6235.0
23	8956.0
24	12635.0
25	17995.0
26	24429.0
27	31330.0
28	42021.0
29	55803.0
30	73133.0
31	100206.0
32	143788.0
33	203922.0
34	449923.0
35	984874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.051048883690214	17.494007465262655	12.080009844302403	25.374933806744732
2	16.667565364108423	19.262727053604305	37.83069185416031	26.239015728126976
3	19.445311142583645	22.83876849081917	28.14835332218327	29.567567044413913
4	13.128645860220209	15.616496065605205	34.93190399634216	36.32295407783243
5	15.183047904707855	36.19608709142184	33.01837601892418	15.602488984946131
6	34.812387104152634	34.658961734043324	16.515385654750954	14.013265507053093
7	30.981544851119114	30.066653717627595	19.700378920871376	19.251422510381914
8	28.252712468010248	33.151925428986786	18.75781721815685	19.83754488484612
9	27.04982175864191	14.879392936603661	18.519890775836693	39.55089452891774
10	16.829122645418945	26.61718381537888	30.386682455400365	26.16701108380181
11	36.80574368213886	21.513240752748366	21.90344156656281	19.777573998549958
12	24.742555391834962	23.392837283009325	28.25556964075661	23.609037684399098
13	29.364777955847277	19.616311248049787	24.920710877226337	26.098199918876603
14	23.703227271007528	19.609918508477204	24.82247840903743	31.864375811477835
15	25.15517115667003	27.003473065056056	21.93564077265776	25.90571500561616
16	25.80405970325555	26.06028279129515	23.28340220703453	24.852255298414764
17	24.195225225266796	26.090433423146266	24.765342760384055	24.948998591202887
18	24.716645981646465	25.334705157721356	25.65905242614525	24.289596434486928
19	25.663093019393653	24.999815474323253	25.070350414260144	24.266741092022954
20	25.880131689353725	24.52988681302815	24.76846651650107	24.821514981117055
21	26.56106925338351	24.30974728675662	24.40265283631786	24.726530623542008
22	25.996432891581495	24.266948147751734	24.82849553096492	24.908123429701853
23	24.670512190149545	24.456957411734535	25.193144845488735	25.67938555262719
24	24.739578287965386	24.925109441238476	25.225917161401036	25.10939510939511
25	25.04731552710397	24.50702133361748	25.117291305428818	25.328371833849733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	511.0
1	511.0
2	583.0
3	655.0
4	655.0
5	655.0
6	1355.5
7	2056.0
8	2056.0
9	2056.0
10	2601.5
11	3147.0
12	3147.0
13	3147.0
14	4345.0
15	5543.0
16	5543.0
17	5543.0
18	9446.5
19	13350.0
20	13350.0
21	13350.0
22	20990.0
23	28630.0
24	28630.0
25	28630.0
26	42431.0
27	56232.0
28	56232.0
29	56232.0
30	71824.0
31	87416.0
32	87416.0
33	87416.0
34	108231.0
35	129046.0
36	129046.0
37	129046.0
38	151954.0
39	174862.0
40	174862.0
41	174862.0
42	201274.5
43	227687.0
44	227687.0
45	227687.0
46	252492.5
47	277298.0
48	277298.0
49	277298.0
50	287875.5
51	298453.0
52	298453.0
53	298453.0
54	281182.5
55	263912.0
56	263912.0
57	263912.0
58	242998.0
59	222084.0
60	222084.0
61	222084.0
62	193916.0
63	165748.0
64	165748.0
65	165748.0
66	135237.5
67	104727.0
68	104727.0
69	104727.0
70	79587.0
71	54447.0
72	54447.0
73	54447.0
74	41055.5
75	27664.0
76	27664.0
77	27664.0
78	21524.5
79	15385.0
80	15385.0
81	15385.0
82	10687.5
83	5990.0
84	5990.0
85	5990.0
86	4429.5
87	2869.0
88	2869.0
89	2869.0
90	1895.0
91	921.0
92	921.0
93	921.0
94	601.5
95	282.0
96	282.0
97	282.0
98	588.5
99	895.0
100	895.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001244348583516529
2	1.382609537240588E-4
3	7.834787377696664E-4
4	0.010415658513879097
5	0.03378175969324503
6	0.06124960249975804
7	0.10779745692019117
8	0.10687571722869744
9	0.11632354906650813
10	0.13199312382190145
11	0.137200953078841
12	0.1308409492075343
13	0.12632442471921504
14	0.11374267793032569
15	0.13125573206870647
16	0.11789050654204747
17	0.12503398915112385
18	0.1096870232877533
19	0.09632179776109429
20	0.0912983164424535
21	0.09295744788714219
22	0.102681801632401
23	0.08056004903655159
24	0.09125222945787881
25	0.08834874942967356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2169810.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.85043410444567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.59828857828349	39.95402368915439
2	13.979540143579412	14.77649530337393
3	4.485706951898833	7.112146790195493
4	1.987936063816071	4.202531357782494
5	1.0572858201385158	2.793900728339771
6	0.6190941296764656	1.9631636102949108
7	0.4129259713680451	1.5276321787860763
8	0.30186978323516117	1.2763159269594546
9	0.22557060205224613	1.0729353815696134
>10	1.1790850678933702	11.288800206548002
>50	0.0846615818540947	3.0955995527122955
>100	0.058406806874154	6.3947872275823405
>500	0.007090076779550781	2.550550813209356
>1k	0.0025384225507033465	1.9911172334919152
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3936	0.18139837128596512	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2357	0.10862702264253553	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	4.6086984574686265E-5
5	0.0	0.0	0.0	4.6086984574686265E-5	4.6086984574686265E-5
6	0.0	0.0	0.0	4.6086984574686265E-5	4.6086984574686265E-5
7	0.0	0.0	0.0	4.6086984574686265E-5	4.6086984574686265E-5
8	0.0	0.0	0.0	4.6086984574686265E-5	4.6086984574686265E-5
9	0.0	0.0	0.0	4.6086984574686265E-5	9.217396914937253E-5
10	0.0	0.0	0.0	4.6086984574686265E-5	9.217396914937253E-5
11	0.0	0.0	0.0	4.6086984574686265E-5	9.217396914937253E-5
12	0.0	0.0	0.0	4.6086984574686265E-5	1.382609537240588E-4
13	0.0	0.0	0.0	4.6086984574686265E-5	1.8434793829874506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1030	0.0	14.842972	1
CAAATCG	115	5.324182E-9	12.391846	13
GTATCAA	2665	0.0	12.185998	1
TTTGCGC	170	1.0913936E-11	11.174639	17
TAGAACC	145	1.2169039E-9	11.138417	4
CGAACGA	120	1.2776763E-7	11.082284	16
GTATACG	60	0.005896237	11.07845	1
CGGTCCA	330	0.0	10.941893	10
GGGTCGA	70	0.0014895478	10.859121	6
GTAATAC	105	3.4725654E-6	10.855864	3
ATTTGCG	160	5.456968E-10	10.686488	16
CTAGACA	205	0.0	10.659002	4
GTCCTAA	250	0.0	10.635311	1
TTGCGCG	170	1.4006218E-10	10.616397	18
CGCCGGT	325	0.0	10.525237	7
CGAGCCG	325	0.0	10.522323	15
AAGACGG	385	0.0	10.365046	5
GTGTTAT	110	6.079501E-6	10.35907	1
GCGCCGA	270	0.0	10.201797	19
AAATCGC	140	9.696123E-8	10.178312	14
>>END_MODULE