FastQCFastQC Report
Thu 2 Feb 2017
SRR4062171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169810
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28950.026.8325861
GGTATCA12800.024.9284041
GTAATAC1452.9649527E-1019.7243023
TATACCG851.4316577E-418.117385
ACCGTCG4800.017.8747378
ATACCGT5550.017.8375766
TCAACGC44050.017.679654
ATCAACG44950.017.277123
CAACGCA44950.017.2767225
TATCAAC45300.017.241162
AACGCAG45000.017.2086376
TAGGACG3100.017.0320074
CGTCTTA1702.4720066E-916.82328215
TACCGTC5550.016.6484057
CGTCGTA5600.016.49975810
CCGTCGT5400.015.8886569
TCGTTTA4900.015.714416530
CGCGATC1004.945632E-415.40012823
CGGATCG1004.945632E-415.40012826
GTAAACG3750.015.25346227
Produced by FastQC (version 0.11.5)