FastQCFastQC Report
Thu 2 Feb 2017
SRR4062170_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062170_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074425
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34510.16635935259168205No Hit
TATCAACGCAGAGTACTTTTTTTTT22640.10913867698277835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA250.00603083719.001048
GGTATCA10150.014.3137231
CGCGTAA1450.013.75937310
TCGCGTA1600.013.063539
GCGTAAC1600.012.47033511
AAGACGG4000.012.3509735
CGTCTTA701.0911734E-412.21436415
GTATCAA23550.012.0964381
CGAACGA951.0399326E-612.00036516
TCTAGAC1701.8189894E-1211.7350853
CAAGACG4550.011.2753414
CCGCTAA600.005896593411.0783211
CGGACCA4050.011.0255599
CCGACCA3800.011.0008669
AGGACTG3350.010.7769695
CGAGCCG2850.010.66673515
GGACCGT1252.2377571E-710.6410956
TTGCGCG2500.010.63878318
CGTCGTA2700.010.55613110
CGCATCG3000.010.45082413
Produced by FastQC (version 0.11.5)