FastQCFastQC Report
Thu 2 Feb 2017
SRR4062170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074425
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25000.025.6152271
GGTATCA12350.024.4117471
ATACGAA4550.018.37703940
TACGAAT4600.018.17728841
ACCGTCG5350.017.6812658
CGCAATA4850.017.23782236
TCTAGCG4900.017.06110428
CGAATGC5050.016.99202743
TCAACGC37850.016.7969574
CCGTCGT5250.016.7609799
CTAGCGG5050.016.55393829
ATACCGT6000.016.4990866
CGTCGTA5250.016.34195310
CAACGCA39050.016.2807885
ATCAACG39250.016.2538783
TACCGTC5550.016.2513547
TATACCG953.349171E-416.209635
AACGCAG39500.016.0953126
CGTCTTA1653.1161107E-815.99911415
TATCAAC40750.015.8179112
Produced by FastQC (version 0.11.5)