##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062170_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2074425
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.924635983465297	32.0	32.0	32.0	32.0	32.0
2	31.299939019246295	32.0	32.0	32.0	32.0	32.0
3	31.47727972811743	32.0	32.0	32.0	32.0	32.0
4	31.59254106559649	32.0	32.0	32.0	32.0	32.0
5	31.518555744362896	32.0	32.0	32.0	32.0	32.0
6	35.1928259638213	36.0	36.0	36.0	36.0	36.0
7	35.199199296190514	36.0	36.0	36.0	36.0	36.0
8	35.13005242416573	36.0	36.0	36.0	36.0	36.0
9	35.25907420128469	36.0	36.0	36.0	36.0	36.0
10	35.09981271918724	36.0	36.0	36.0	36.0	36.0
11	35.277015076466974	36.0	36.0	36.0	36.0	36.0
12	35.17993468069465	36.0	36.0	36.0	36.0	36.0
13	35.227652482013085	36.0	36.0	36.0	36.0	36.0
14	35.17999879484677	36.0	36.0	36.0	36.0	36.0
15	35.144121865095144	36.0	36.0	36.0	36.0	36.0
16	35.1653947479422	36.0	36.0	36.0	36.0	36.0
17	35.1380208973571	36.0	36.0	36.0	36.0	36.0
18	35.14698289887559	36.0	36.0	36.0	36.0	36.0
19	35.14302710389626	36.0	36.0	36.0	36.0	36.0
20	35.14888607686467	36.0	36.0	36.0	36.0	36.0
21	35.13370596671367	36.0	36.0	36.0	36.0	36.0
22	35.120850838184076	36.0	36.0	36.0	36.0	36.0
23	35.08324379044795	36.0	36.0	36.0	36.0	36.0
24	35.066368752786914	36.0	36.0	36.0	36.0	36.0
25	35.05163792376201	36.0	36.0	36.0	36.0	36.0
26	35.00846162189522	36.0	36.0	36.0	36.0	36.0
27	34.994418694336986	36.0	36.0	36.0	36.0	36.0
28	34.96976559769575	36.0	36.0	36.0	36.0	36.0
29	34.951692637718885	36.0	36.0	36.0	36.0	36.0
30	34.937044241175265	36.0	36.0	36.0	32.0	36.0
31	34.93422080817576	36.0	36.0	36.0	32.0	36.0
32	34.90772382708462	36.0	36.0	36.0	32.0	36.0
33	34.8867903153886	36.0	36.0	36.0	32.0	36.0
34	34.87302457307447	36.0	36.0	36.0	32.0	36.0
35	34.86852742326187	36.0	36.0	36.0	32.0	36.0
36	34.84267977873387	36.0	36.0	36.0	32.0	36.0
37	34.834985116357544	36.0	36.0	36.0	32.0	36.0
38	34.815023440230426	36.0	36.0	36.0	32.0	36.0
39	34.80016100847223	36.0	36.0	36.0	32.0	36.0
40	34.788734227557036	36.0	36.0	36.0	32.0	36.0
41	34.76168094773251	36.0	36.0	36.0	32.0	36.0
42	34.72930185473083	36.0	36.0	36.0	32.0	36.0
43	34.72577268399677	36.0	36.0	36.0	32.0	36.0
44	34.6827834219121	36.0	36.0	36.0	32.0	36.0
45	34.66209768972125	36.0	36.0	36.0	32.0	36.0
46	34.648279402726054	36.0	36.0	36.0	32.0	36.0
47	34.61948539956855	36.0	36.0	36.0	32.0	36.0
48	34.594847246827435	36.0	36.0	36.0	32.0	36.0
49	34.57966520843125	36.0	36.0	36.0	32.0	36.0
50	34.1397842775709	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	24.0
21	47.0
22	140.0
23	321.0
24	926.0
25	2168.0
26	4580.0
27	8512.0
28	14960.0
29	24824.0
30	38770.0
31	60589.0
32	94802.0
33	168698.0
34	384848.0
35	1270209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38373999421352	18.231362715787444	12.417880219886198	25.967017070112835
2	16.42403433972961	19.407259782908426	36.91588424610911	27.252821631252854
3	18.758794631171426	22.77370193774739	28.409834271668355	30.057669159412832
4	12.946189908046806	15.635899104571147	34.84729503356352	36.57061595381853
5	15.100859274256722	36.25481760005785	32.719331863046385	15.924991262639043
6	35.58163940685136	34.46619562759922	16.029340235178875	13.922824730370548
7	31.384696964219	29.950853851067162	19.55283994359883	19.11160924111501
8	28.638008122732806	33.03874567651277	18.471383636429373	19.851862564325053
9	27.051785434518	14.39083119418634	18.325222652060212	40.23216071923545
10	16.6680485763759	26.38713204243684	30.606905820603696	26.337913560583566
11	37.51550429637128	21.124745411379056	21.505091772418865	19.854658519830796
12	24.599069428429292	23.371933606630083	28.092376486195686	23.936620478744945
13	29.63004205984791	19.12120226086747	25.033346590018922	26.2154090892657
14	23.74764091254203	19.100618243609674	24.721597551128628	32.43014329271967
15	25.51063547730094	26.77855309302578	21.72987695385468	25.9809344758186
16	26.147627518185896	25.772243674104928	22.981088598685893	25.099040209023286
17	24.505103823951217	25.972739433819008	24.553020716584115	24.96913602564566
18	25.20356243296335	25.0012895139617	25.340419634356508	24.454728418718442
19	25.846101931860638	24.982248092845005	24.983742482856695	24.187907492437663
20	26.142886273216742	24.192267810890662	24.562732986923088	25.10211292896951
21	26.776155694303576	24.0535686050682	24.404220159427386	24.766055541200835
22	26.23170276100606	23.93280065560336	24.8034033527363	25.03209323065428
23	24.90917960841178	24.001313135598124	25.25978295661832	25.829724299371776
24	25.041361197989982	24.63661921859152	24.927690213304228	25.39432937011427
25	25.163190271589297	24.219793590398083	25.111754182737435	25.505261955275188
26	25.04624195255048	24.908178932373705	25.207444026388163	24.838135088687654
27	25.450560108907656	24.624256056397506	24.69743320777201	25.22775062692283
28	25.182330269789503	24.42933963878625	25.179003998756265	25.209326092667983
29	25.00179568697681	24.705181942861	25.16140695087531	25.131615419286884
30	25.047748700589374	24.72539073911421	25.21526685762933	25.011593702667085
31	25.451537374669364	24.528818513960335	24.62720779324079	25.392436318129512
32	25.432991002734273	24.467462524247882	24.502219428386315	25.59732704463153
33	24.999590246517336	24.37527055781429	24.914313315831237	25.71082587983714
34	25.504517190197305	24.405264342759793	25.109850564666854	24.98036790237605
35	25.872931167714913	24.323726306016287	25.052942534753104	24.7503999915157
36	25.00085566044694	24.783445597590845	24.788459044716575	25.42723969724564
37	25.88009005056908	24.467626627586906	24.657176326534547	24.995106995309467
38	25.210166672692434	24.30895308338455	25.09085650240428	25.390023741518736
39	25.579972695824544	24.429523989494832	24.552402420353182	25.43810089432744
40	25.75999483229545	24.563709794824774	24.82542124987105	24.850874123008722
41	25.052147382700323	24.85035496857177	25.051279665678273	25.04621798304963
42	25.906742495496115	24.79119673377457	24.672603709840473	24.62945706088884
43	25.508752426676644	24.11977984312111	24.914249792343487	25.45721793785876
44	25.180719697061	24.60805700112566	24.769698386719664	25.441524915093684
45	25.362431672717438	24.801351336368118	24.71404860510868	25.12216838580576
46	25.158782306625447	24.570414017482804	24.854691592335257	25.41611208355649
47	25.290228779626666	24.62921772967386	24.992021781741016	25.088531708958456
48	25.877237807276682	25.106442879511288	24.16672772991276	24.849591583299265
49	25.270121672259606	25.02643005520302	24.37909256087349	25.324355711663888
50	25.34642563942617	25.127904515958598	24.3392423540505	25.18642749056474
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	94.0
1	77.0
2	60.0
3	153.5
4	247.0
5	276.0
6	305.0
7	242.5
8	180.0
9	260.5
10	341.0
11	614.5
12	888.0
13	1573.0
14	2258.0
15	2913.5
16	3569.0
17	3598.5
18	3628.0
19	3581.5
20	3535.0
21	4221.5
22	4908.0
23	6035.0
24	7162.0
25	9161.0
26	11160.0
27	13857.0
28	16554.0
29	19426.0
30	22298.0
31	25393.0
32	28488.0
33	32670.0
34	36852.0
35	42994.0
36	49136.0
37	58302.5
38	67469.0
39	75428.0
40	83387.0
41	92225.0
42	101063.0
43	107286.0
44	113509.0
45	123744.0
46	133979.0
47	144054.0
48	154129.0
49	159729.0
50	165329.0
51	163597.0
52	161865.0
53	160813.5
54	159762.0
55	160495.5
56	161229.0
57	154765.0
58	148301.0
59	135655.5
60	123010.0
61	107917.0
62	92824.0
63	79912.5
64	67001.0
65	56825.5
66	46650.0
67	40237.5
68	33825.0
69	29937.0
70	26049.0
71	20589.5
72	15130.0
73	13237.5
74	11345.0
75	8547.5
76	5750.0
77	5028.0
78	4306.0
79	3694.5
80	3083.0
81	2365.0
82	1647.0
83	1363.5
84	1080.0
85	832.5
86	585.0
87	404.5
88	224.0
89	150.0
90	76.0
91	64.0
92	52.0
93	42.0
94	32.0
95	31.5
96	31.0
97	28.0
98	25.0
99	20.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0301288308808465
2	0.004483170035069959
3	2.892367764561264E-4
4	0.0
5	0.0
6	1.928245176374176E-4
7	0.0
8	0.0
9	0.0
10	4.82061294093544E-5
11	0.0
12	4.82061294093544E-5
13	0.0
14	0.0
15	0.0
16	7.23091941140316E-4
17	0.0
18	0.0
19	0.0
20	2.892367764561264E-4
21	4.82061294093544E-5
22	0.0
23	4.82061294093544E-5
24	8.195041999590248E-4
25	2.892367764561264E-4
26	4.8206129409354405E-4
27	3.374429058654808E-4
28	0.001446183882280632
29	4.8206129409354405E-4
30	0.0011087409764151512
31	5.784735529122528E-4
32	8.195041999590248E-4
33	3.374429058654808E-4
34	7.712980705496704E-4
35	2.892367764561264E-4
36	1.928245176374176E-4
37	0.0016872145293274038
38	0.0
39	8.195041999590248E-4
40	1.446183882280632E-4
41	7.712980705496704E-4
42	0.0050134374585728576
43	0.004145727129204479
44	0.0038564903527483524
45	0.0016390083999180496
46	0.0021210696940115936
47	0.0013015654940525688
48	0.0035190474468828714
49	0.00395290261156706
50	0.0012533593646432144
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2074425.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.94263865566905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.69916682242409	42.14054607915327
2	13.688086035972033	15.041191299242415
3	4.128255128064584	6.804516893389955
4	1.766757286595603	3.882812287587701
5	0.95999080487568	2.637221395252469
6	0.5876131087821522	1.9371008823091285
7	0.4062907131612585	1.5625878689661175
8	0.2733501578630693	1.201486315995257
9	0.2117226647044247	1.0469341675863524
>10	1.1394805734340703	11.209951613460408
>50	0.076852611672983	2.9335811417090345
>100	0.054588127145919506	5.977381867743056
>500	0.006082827033537847	2.2965319132370707
>1k	0.0017631382705906727	1.3281562743677715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.64122588187088E-5
2	0.0	0.0	0.0	0.0	9.64122588187088E-5
3	0.0	0.0	0.0	0.0	9.64122588187088E-5
4	0.0	0.0	0.0	0.0	9.64122588187088E-5
5	0.0	0.0	0.0	0.0	9.64122588187088E-5
6	0.0	0.0	0.0	0.0	1.446183882280632E-4
7	0.0	0.0	0.0	0.0	1.446183882280632E-4
8	0.0	0.0	0.0	0.0	1.446183882280632E-4
9	0.0	0.0	0.0	0.0	1.446183882280632E-4
10	0.0	0.0	0.0	0.0	1.446183882280632E-4
11	0.0	0.0	0.0	0.0	1.446183882280632E-4
12	0.0	0.0	0.0	0.0	2.892367764561264E-4
13	0.0	0.0	0.0	0.0	3.374429058654808E-4
14	0.0	0.0	0.0	0.0	3.374429058654808E-4
15	0.0	0.0	0.0	0.0	4.338551646841896E-4
16	0.0	0.0	0.0	9.64122588187088E-5	4.338551646841896E-4
17	0.0	0.0	0.0	9.64122588187088E-5	4.338551646841896E-4
18	0.0	0.0	0.0	1.446183882280632E-4	4.338551646841896E-4
19	0.0	0.0	0.0	1.928245176374176E-4	4.338551646841896E-4
20	0.0	0.0	0.0	2.41030647046772E-4	4.338551646841896E-4
21	0.0	0.0	0.0	3.374429058654808E-4	4.338551646841896E-4
22	0.0	0.0	0.0	4.82061294093544E-4	4.338551646841896E-4
23	0.0	0.0	0.0	0.0010123287175964424	5.302674235028984E-4
24	0.0	0.0	0.0	0.0013015654940525688	5.302674235028984E-4
25	0.0	0.0	0.0	0.0016390083999180496	5.784735529122528E-4
26	0.0	0.0	0.0	0.002072863564602239	6.266796823216072E-4
27	0.0	0.0	0.0	0.0022174819528303024	6.266796823216072E-4
28	0.0	0.0	0.0	0.002940573893970618	6.266796823216072E-4
29	0.0	0.0	0.0	0.003904696482157706	6.748858117309616E-4
30	0.0	0.0	0.0	0.005736529399713174	6.748858117309616E-4
31	0.0	0.0	0.0	0.008628897164274438	6.748858117309616E-4
32	0.0	0.0	0.0	0.012919242681706978	6.748858117309616E-4
33	0.0	0.0	0.0	0.017788061752051772	6.748858117309616E-4
34	0.0	0.0	0.0	0.023042529857671402	7.23091941140316E-4
35	0.0	0.0	0.0	0.029212914422068765	7.23091941140316E-4
36	0.0	0.0	0.0	0.036636658351109345	7.23091941140316E-4
37	0.0	0.0	0.0	0.048157923279945045	7.23091941140316E-4
38	0.0	0.0	0.0	0.0644998011497162	7.23091941140316E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2500	0.0	25.615227	1
GGTATCA	1235	0.0	24.411747	1
ATACGAA	455	0.0	18.377039	40
TACGAAT	460	0.0	18.177288	41
ACCGTCG	535	0.0	17.681265	8
CGCAATA	485	0.0	17.237822	36
TCTAGCG	490	0.0	17.061104	28
CGAATGC	505	0.0	16.992027	43
TCAACGC	3785	0.0	16.796957	4
CCGTCGT	525	0.0	16.760979	9
CTAGCGG	505	0.0	16.553938	29
ATACCGT	600	0.0	16.499086	6
CGTCGTA	525	0.0	16.341953	10
CAACGCA	3905	0.0	16.280788	5
ATCAACG	3925	0.0	16.253878	3
TACCGTC	555	0.0	16.251354	7
TATACCG	95	3.349171E-4	16.20963	5
AACGCAG	3950	0.0	16.095312	6
CGTCTTA	165	3.1161107E-8	15.999114	15
TATCAAC	4075	0.0	15.817911	2
>>END_MODULE