FastQCFastQC Report
Thu 2 Feb 2017
SRR4062169_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062169_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4727753
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89130.1885250773464688No Hit
TATCAACGCAGAGTACTTTTTTTTT54330.11491717101126053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.014.2887841
CGGTCCA5800.013.4324510
GTATCAA62800.012.6717781
CGAATTT951.0404619E-612.00075615
CGCATCG6750.011.682713513
CGTCTTA3750.011.65406815
TATACCG1104.97379E-711.2276245
ATCGCCA7300.010.93173316
CGCCGGT6100.010.9026737
AAGACGG10050.010.7764565
GCCGGTC6450.010.7529578
GGGTAGG11400.010.7457811
GCATCGC7550.010.69637914
CGTCGTA7400.010.65652910
CAAGACG9850.010.6087944
ATTTGCG5250.010.49545516
ACGCCCC4650.010.41901817
TTGCGCG5400.010.37907418
CCGGTCC7650.010.30816659
CCGTCGT7450.010.202319
Produced by FastQC (version 0.11.5)