##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062169_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4727753
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24630474561594	32.0	32.0	32.0	32.0	32.0
2	30.76267330378723	32.0	32.0	32.0	32.0	32.0
3	30.77961813994936	32.0	32.0	32.0	32.0	32.0
4	30.778392187578326	32.0	32.0	32.0	32.0	32.0
5	30.694907919258895	32.0	32.0	32.0	32.0	32.0
6	34.34451313340608	36.0	36.0	36.0	32.0	36.0
7	34.231999641267215	36.0	36.0	36.0	32.0	36.0
8	34.220734458843346	36.0	36.0	36.0	32.0	36.0
9	34.30505337313519	36.0	36.0	36.0	32.0	36.0
10	34.00412775371302	36.0	36.0	36.0	32.0	36.0
11	34.36882912453337	36.0	36.0	36.0	32.0	36.0
12	34.13062061406338	36.0	36.0	36.0	32.0	36.0
13	34.22419276133927	36.0	36.0	36.0	32.0	36.0
14	34.1409303743237	36.0	36.0	36.0	32.0	36.0
15	34.06363509261165	36.0	36.0	36.0	32.0	36.0
16	34.08085532387162	36.0	36.0	36.0	32.0	36.0
17	34.04522105956043	36.0	36.0	36.0	32.0	36.0
18	34.050186843517416	36.0	36.0	36.0	32.0	36.0
19	34.031201714641185	36.0	36.0	36.0	32.0	36.0
20	34.04925066939834	36.0	36.0	36.0	32.0	36.0
21	34.03257319068911	36.0	36.0	36.0	32.0	36.0
22	33.9698296421154	36.0	36.0	36.0	32.0	36.0
23	33.93807713727853	36.0	36.0	36.0	32.0	36.0
24	33.93909770666953	36.0	36.0	36.0	32.0	36.0
25	33.553085577863314	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	72.0
5	383.0
6	990.0
7	234.0
8	790.0
9	613.0
10	371.0
11	90.0
12	187.0
13	222.0
14	697.0
15	1032.0
16	1559.0
17	2218.0
18	3028.0
19	4079.0
20	6413.0
21	9433.0
22	14101.0
23	21083.0
24	30412.0
25	42276.0
26	58415.0
27	75276.0
28	98884.0
29	129966.0
30	170111.0
31	229773.0
32	325617.0
33	459388.0
34	994518.0
35	2045514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.619499795990066	17.675382441947065	12.258680786537079	25.44643697552579
2	16.59934074320374	19.376899953212394	37.7588653202328	26.264893983351058
3	19.000343717115527	23.120993712620763	28.181503788820205	29.697158781443505
4	12.866065881334569	15.717296521005734	35.47023146827007	35.94640612938962
5	15.045169977749321	36.36765078029719	33.10512070294879	15.482058539004703
6	34.56085537176789	35.195933098204016	16.540614835496964	13.702596694531127
7	30.83516030251815	30.390928351611496	19.926830889916445	18.847080455953915
8	28.1508491085419	33.094291280227	18.847626307542455	19.907233303688646
9	26.92186823011807	15.013101938460066	18.686642375739115	39.37838745568275
10	16.253852822141255	26.794293131963965	30.78191242304681	26.169941622847976
11	36.727713531112386	21.75832168886793	21.994430567108235	19.519534212911452
12	24.661462370729204	23.810755262540336	28.24940209476512	23.27838027196534
13	28.892923220808793	19.86383706193707	25.20583705515984	26.0374026620943
14	23.550229221852746	19.82025211968538	24.99459477316153	31.63492388530035
15	25.12822902359134	27.216280643490805	22.162012184854817	25.493478148063037
16	25.674577047915264	26.345887545538467	23.30889579588095	24.670639610665322
17	23.907549250818306	26.399124768739707	24.905420470178434	24.787905510263553
18	24.592947270970107	25.6006584670271	25.75064309376167	24.05575116824112
19	25.213720363548518	25.448247987396442	25.52857639805628	23.80945525099876
20	25.612440474010327	24.691379457449436	24.965866437789902	24.730313630750334
21	26.395080531401184	24.553413118455904	24.753458128053786	24.29804822208913
22	25.699931313271613	24.46566637560207	25.159690291457014	24.674712019669304
23	24.392745482862153	24.68568493914806	25.43338125335664	25.488188324633143
24	24.43486189935519	25.26564004807551	25.364530614897774	24.934967437671528
25	24.621906415619048	24.84358215805601	25.266482991875204	25.268028434449736
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	444.0
1	444.0
2	979.5
3	1515.0
4	1515.0
5	1515.0
6	3340.5
7	5166.0
8	5166.0
9	5166.0
10	6223.0
11	7280.0
12	7280.0
13	7280.0
14	10785.5
15	14291.0
16	14291.0
17	14291.0
18	24329.5
19	34368.0
20	34368.0
21	34368.0
22	53784.0
23	73200.0
24	73200.0
25	73200.0
26	104146.5
27	135093.0
28	135093.0
29	135093.0
30	168439.0
31	201785.0
32	201785.0
33	201785.0
34	247780.5
35	293776.0
36	293776.0
37	293776.0
38	343540.5
39	393305.0
40	393305.0
41	393305.0
42	446833.0
43	500361.0
44	500361.0
45	500361.0
46	551538.0
47	602715.0
48	602715.0
49	602715.0
50	621258.5
51	639802.0
52	639802.0
53	639802.0
54	599492.0
55	559182.0
56	559182.0
57	559182.0
58	512274.5
59	465367.0
60	465367.0
61	465367.0
62	405797.5
63	346228.0
64	346228.0
65	346228.0
66	282898.5
67	219569.0
68	219569.0
69	219569.0
70	167045.0
71	114521.0
72	114521.0
73	114521.0
74	87138.0
75	59755.0
76	59755.0
77	59755.0
78	47602.0
79	35449.0
80	35449.0
81	35449.0
82	24428.0
83	13407.0
84	13407.0
85	13407.0
86	9962.5
87	6518.0
88	6518.0
89	6518.0
90	4346.0
91	2174.0
92	2174.0
93	2174.0
94	1387.5
95	601.0
96	601.0
97	601.0
98	1241.0
99	1881.0
100	1881.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.883712833559622E-4
2	1.0575848611380501E-4
3	5.92247522237308E-4
4	0.010491241822489457
5	0.03394847404253141
6	0.0629686026321595
7	0.10565272762769122
8	0.10899469578888744
9	0.11741307128354632
10	0.13143664654223688
11	0.1401511457980144
12	0.13259998988948873
13	0.12811583007826338
14	0.1157420872029482
15	0.13363642305340404
16	0.11942248251970862
17	0.12270099558923657
18	0.11144829266672772
19	0.09818617850805658
20	0.09298286099125737
21	0.09129072521343648
22	0.10281840019984125
23	0.08270313614099552
24	0.09200988291901037
25	0.08856215627170032
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4727753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.12459942085281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.39471977092512	28.38975573430849
2	16.48181494350409	13.885797044474712
3	6.534689062743284	8.258134773236666
4	3.202152413215796	5.395575507649302
5	1.7744148403614497	3.737325717832128
6	1.1090641489861235	2.8031329804841683
7	0.7153836377659545	2.109467442118631
8	0.4919510377812148	1.6578592320965198
9	0.34327502567817925	1.3014290653088636
>10	1.7331729095093389	13.132477327429978
>50	0.12263039847004054	3.569831067704428
>100	0.07819072095201038	6.638927466012332
>500	0.010861947254914999	3.1394881308999825
>1k	0.007578101499252423	5.675492208182161
>5k	1.0104135332335416E-4	0.30530630226137917
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8913	0.1885250773464688	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5433	0.11491717101126053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.48061880559327E-4
2	0.0	0.0	0.0	0.0	1.48061880559327E-4
3	0.0	0.0	0.0	0.0	1.48061880559327E-4
4	0.0	0.0	0.0	0.0	1.48061880559327E-4
5	0.0	0.0	0.0	0.0	2.1151697222761002E-4
6	0.0	2.1151697222761003E-5	0.0	0.0	2.5382036667313205E-4
7	0.0	2.1151697222761003E-5	0.0	0.0	2.7497206389589303E-4
8	0.0	2.1151697222761003E-5	0.0	0.0	2.7497206389589303E-4
9	0.0	2.1151697222761003E-5	0.0	0.0	2.7497206389589303E-4
10	0.0	4.2303394445522006E-5	0.0	2.1151697222761003E-5	2.96123761118654E-4
11	0.0	4.2303394445522006E-5	0.0	2.1151697222761003E-5	2.96123761118654E-4
12	0.0	4.2303394445522006E-5	0.0	2.1151697222761003E-5	5.710958250145471E-4
13	0.0	4.2303394445522006E-5	0.0	2.1151697222761003E-5	7.191577055738741E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2140	0.0	14.288784	1
CGGTCCA	580	0.0	13.43245	10
GTATCAA	6280	0.0	12.671778	1
CGAATTT	95	1.0404619E-6	12.000756	15
CGCATCG	675	0.0	11.6827135	13
CGTCTTA	375	0.0	11.654068	15
TATACCG	110	4.97379E-7	11.227624	5
ATCGCCA	730	0.0	10.931733	16
CGCCGGT	610	0.0	10.902673	7
AAGACGG	1005	0.0	10.776456	5
GCCGGTC	645	0.0	10.752957	8
GGGTAGG	1140	0.0	10.745781	1
GCATCGC	755	0.0	10.696379	14
CGTCGTA	740	0.0	10.656529	10
CAAGACG	985	0.0	10.608794	4
ATTTGCG	525	0.0	10.495455	16
ACGCCCC	465	0.0	10.419018	17
TTGCGCG	540	0.0	10.379074	18
CCGGTCC	765	0.0	10.3081665	9
CCGTCGT	745	0.0	10.20231	9
>>END_MODULE