FastQCFastQC Report
Thu 2 Feb 2017
SRR4062169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4727753
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.027.8611811
GTATCAA62200.027.562591
CGCAATA10750.018.62339436
TCTAGCG11150.017.75722928
ATACGAA11500.017.60106340
TCAACGC97400.017.391594
ATCAACG98600.017.1799283
CAACGCA99150.017.1290055
TACGAAT11800.016.96712541
TATCAAC101750.016.9300352
AACGCAG100900.016.8537246
CTAGCGG11750.016.6632529
CAATACG12050.016.24994338
CGAATGC12500.016.1933243
TATACCG2900.015.9304795
TAGCGGC12750.015.70141330
ACCGTCG12900.015.6893758
ATACCGT13700.015.57616
AATACGA12800.015.46968339
GTCCTAT12600.015.3703961
Produced by FastQC (version 0.11.5)