##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4727753
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2718433048427	32.0	32.0	32.0	32.0	32.0
2	31.46037980410567	32.0	32.0	32.0	32.0	32.0
3	31.581395855494144	32.0	32.0	32.0	32.0	32.0
4	31.6538784915371	32.0	32.0	32.0	32.0	32.0
5	31.626533154333572	32.0	32.0	32.0	32.0	32.0
6	35.271268190195215	36.0	36.0	36.0	36.0	36.0
7	35.27559011648874	36.0	36.0	36.0	36.0	36.0
8	35.22811238235162	36.0	36.0	36.0	36.0	36.0
9	35.31049528179666	36.0	36.0	36.0	36.0	36.0
10	35.19358435180518	36.0	36.0	36.0	36.0	36.0
11	35.31717794901722	36.0	36.0	36.0	36.0	36.0
12	35.23706335758234	36.0	36.0	36.0	36.0	36.0
13	35.27142238606797	36.0	36.0	36.0	36.0	36.0
14	35.2363668321928	36.0	36.0	36.0	36.0	36.0
15	35.2167135212013	36.0	36.0	36.0	36.0	36.0
16	35.2283539347339	36.0	36.0	36.0	36.0	36.0
17	35.20790806964746	36.0	36.0	36.0	36.0	36.0
18	35.204190235826616	36.0	36.0	36.0	36.0	36.0
19	35.198104469501686	36.0	36.0	36.0	36.0	36.0
20	35.200199333594625	36.0	36.0	36.0	36.0	36.0
21	35.1932186389602	36.0	36.0	36.0	36.0	36.0
22	35.17608428359096	36.0	36.0	36.0	36.0	36.0
23	35.14664598594724	36.0	36.0	36.0	36.0	36.0
24	35.13119276747326	36.0	36.0	36.0	36.0	36.0
25	35.11741899375877	36.0	36.0	36.0	36.0	36.0
26	35.074518063866705	36.0	36.0	36.0	36.0	36.0
27	35.06250072708959	36.0	36.0	36.0	36.0	36.0
28	35.047292656786425	36.0	36.0	36.0	36.0	36.0
29	35.0274673824965	36.0	36.0	36.0	36.0	36.0
30	35.00749848818244	36.0	36.0	36.0	36.0	36.0
31	35.01124847258306	36.0	36.0	36.0	36.0	36.0
32	34.9878957297473	36.0	36.0	36.0	36.0	36.0
33	34.96583112527241	36.0	36.0	36.0	36.0	36.0
34	34.95411498866375	36.0	36.0	36.0	36.0	36.0
35	34.94558472069078	36.0	36.0	36.0	36.0	36.0
36	34.92085838663737	36.0	36.0	36.0	36.0	36.0
37	34.910184182633905	36.0	36.0	36.0	36.0	36.0
38	34.89906346630207	36.0	36.0	36.0	32.0	36.0
39	34.890002713762755	36.0	36.0	36.0	32.0	36.0
40	34.87487734659573	36.0	36.0	36.0	32.0	36.0
41	34.84558118835734	36.0	36.0	36.0	32.0	36.0
42	34.812970770681126	36.0	36.0	36.0	32.0	36.0
43	34.809800554301376	36.0	36.0	36.0	32.0	36.0
44	34.76653179639461	36.0	36.0	36.0	32.0	36.0
45	34.754351380031906	36.0	36.0	36.0	32.0	36.0
46	34.73333886097687	36.0	36.0	36.0	32.0	36.0
47	34.71140560854173	36.0	36.0	36.0	32.0	36.0
48	34.684806291699246	36.0	36.0	36.0	32.0	36.0
49	34.672560939626074	36.0	36.0	36.0	32.0	36.0
50	34.25805028308374	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	8.0
19	14.0
20	60.0
21	143.0
22	351.0
23	916.0
24	2162.0
25	5152.0
26	10761.0
27	20539.0
28	34994.0
29	55830.0
30	84110.0
31	124988.0
32	191354.0
33	327625.0
34	760263.0
35	3108483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.43529706490786	18.16262161137974	12.641934161803198	25.760147161909202
2	16.029652718990864	19.8852423022105	37.43879991352771	26.646305065270926
3	18.39469141591784	23.430904286382983	28.497491084004427	29.67691321369475
4	12.575011913703541	16.068788761839937	35.6946826068812	35.66151671757532
5	14.72135171218795	36.66053126306117	33.00431156287386	15.61380546187702
6	34.72378568881297	35.27428104156276	16.435583836850657	13.566349432773617
7	30.873969092716987	30.364424706620674	19.92240288356858	18.839203317093762
8	28.267403140561697	33.12956493285499	18.75155068380264	19.85148124278066
9	27.00361038319895	14.721560115344435	18.64879573869447	39.62603376276214
10	16.236863724547078	26.732262379466924	30.887285024497924	26.14358887148807
11	36.97041702474728	21.602609104155825	21.972340771609684	19.45463309948722
12	24.68162980664591	23.619200757400293	28.367208848350938	23.331960587602858
13	28.998300038094204	19.682902215915256	25.28387164050237	26.034926105488164
14	23.664032363788888	19.607898297563345	25.069097306902457	31.658972031745314
15	25.139997795993153	27.178619526020075	22.157820004556076	25.5235626734307
16	25.84472886565011	26.192824040289945	23.354379374456663	24.608067719603277
17	24.047533786134768	26.326502251703925	24.89639369907861	24.729570263082696
18	24.72107773865417	25.41948592470289	25.792517414728483	24.066918921914457
19	25.355448983904193	25.238733918629	25.542303077170065	23.863514020296744
20	25.71242798622006	24.50335160069048	25.050674307243142	24.733546105846322
21	26.592469317341518	24.42657736700233	24.749711913822892	24.23124140183326
22	25.840605463102662	24.365126519934524	25.177119024619092	24.617148992343722
23	24.517566801818962	24.495907463862853	25.504293477260763	25.482232257057426
24	24.553810332216653	25.110783302994395	25.387069986280846	24.948336378508102
25	24.807905004908264	24.562396528234746	25.473466280604747	25.156232186252243
26	24.560249066518775	25.376454211775496	25.671204359467847	24.392092362237886
27	25.041119116835826	24.978975101782506	25.15195459637272	24.82795118500895
28	24.705183341356996	24.787676164481383	25.66753615512373	24.839604339037887
29	24.507433471870367	25.192454656424417	25.586469805744272	24.713642065960943
30	24.596643358655008	25.10054567737137	25.690240205495186	24.612570758478434
31	25.08720374996748	24.818131188367502	24.967801610966468	25.12686345069855
32	24.903468247584087	24.878149483926602	24.92364711185246	25.29473515663685
33	24.539458418795075	24.716561729482677	25.36547519760461	25.378504654117634
34	25.11877302248549	24.863173858830322	25.444257955481664	24.57379516320252
35	25.530950914431916	24.65120784881753	25.44617475057871	24.37166648617184
36	24.56925562413886	25.127809319705413	25.126032574508024	25.1769024816477
37	25.485143639406456	24.8355004323481	25.073143822160247	24.60621210608519
38	24.723034388640862	24.699175274173587	25.474004246837765	25.103786090347786
39	25.315553664111384	24.84355044564803	24.794753160190044	25.04614273005054
40	25.35923544164464	24.905404242061074	25.326598345216723	24.408761971077567
41	24.585458839684524	25.391453809328308	25.484903114560854	24.538184236426318
42	25.53922297570998	25.19498161500684	25.033796954286803	24.231998454996376
43	25.06210412848617	24.464478807600734	25.361160044200542	25.112257019712565
44	24.741213024879553	25.033902431646755	25.000481223097776	25.224403320375917
45	24.902864063838866	25.243392440181207	25.070748635739555	24.782994860240375
46	24.837038589761136	25.106199630133595	25.000037016253096	25.056724763852166
47	24.910759989000994	25.0237747742031	25.32544366182287	24.74002157497303
48	25.522042990282557	25.40354684637806	24.57159899991116	24.502811163428223
49	24.841483112261432	25.486274984283686	24.713891189811598	24.95835071364328
50	24.9685680925951	25.53487122647895	24.735092053607687	24.761468627318262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	115.0
1	116.5
2	118.0
3	337.5
4	557.0
5	576.0
6	595.0
7	503.0
8	411.0
9	598.5
10	786.0
11	1376.5
12	1967.0
13	3603.0
14	5239.0
15	6875.5
16	8512.0
17	8895.0
18	9278.0
19	9329.5
20	9381.0
21	11372.5
22	13364.0
23	16339.5
24	19315.0
25	25045.5
26	30776.0
27	38863.0
28	46950.0
29	54803.5
30	62657.0
31	70882.0
32	79107.0
33	90268.0
34	101429.0
35	115708.0
36	129987.0
37	152719.5
38	175452.0
39	193518.0
40	211584.0
41	230968.0
42	250352.0
43	259124.0
44	267896.0
45	289779.5
46	311663.0
47	329218.5
48	346774.0
49	356850.5
50	366927.0
51	359415.0
52	351903.0
53	349391.0
54	346879.0
55	344548.5
56	342218.0
57	327825.0
58	313432.0
59	287820.0
60	262208.0
61	230166.5
62	198125.0
63	171293.5
64	144462.0
65	121659.0
66	98856.0
67	85609.0
68	72362.0
69	64322.0
70	56282.0
71	43802.0
72	31322.0
73	27655.5
74	23989.0
75	17704.5
76	11420.0
77	10164.5
78	8909.0
79	7518.5
80	6128.0
81	4785.0
82	3442.0
83	2909.0
84	2376.0
85	1847.5
86	1319.0
87	904.0
88	489.0
89	326.0
90	163.0
91	124.0
92	85.0
93	71.5
94	58.0
95	63.0
96	68.0
97	53.0
98	38.0
99	33.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03236209675082433
2	0.004801435269566748
3	2.96123761118654E-4
4	8.460678889104401E-5
5	2.1151697222761003E-5
6	2.3266866945037106E-4
7	0.0
8	0.0
9	0.0
10	1.0575848611380501E-4
11	0.0
12	4.2303394445522006E-5
13	0.0
14	0.0
15	0.0
16	4.8648903612350306E-4
17	0.0
18	4.2303394445522006E-5
19	0.0
20	2.5382036667313205E-4
21	2.1151697222761003E-5
22	0.0
23	0.0
24	0.0011633433472518552
25	2.96123761118654E-4
26	4.2303394445522005E-4
27	5.28792430569025E-4
28	0.0014594671083705093
29	3.3842715556417605E-4
30	0.0010787365583608112
31	6.768543111283521E-4
32	7.191577055738741E-4
33	8.460678889104401E-5
34	8.037644944649181E-4
35	1.9036527500484904E-4
36	1.48061880559327E-4
37	0.0017132874750436411
38	0.0
39	6.55702613905591E-4
40	8.460678889104401E-5
41	0.001184495044474616
42	0.005414834489026817
43	0.0044207047195570495
44	0.004568766600116377
45	0.0021151697222761
46	0.0021151697222761
47	0.0011210399528063333
48	0.0036592436195376533
49	0.0038284571973197417
50	0.0015440738972615532
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4727753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.49332146665086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.1584636288393	28.112928627199974
2	17.08497540343254	14.519947041357636
3	6.86243975318574	8.748235754329375
4	3.3747110162778564	5.736107202869721
5	1.8697043520980665	3.972497404064966
6	1.1066783347086366	2.8215862942171164
7	0.7455103091985427	2.217544645783317
8	0.47139608972422636	1.6024948463019
9	0.3563169458367322	1.3626981471109934
>10	1.7610852890441933	13.289423126376498
>50	0.11833653634333963	3.456471802846471
>100	0.07363313188603224	6.178061502473448
>500	0.010129762294058	2.922523563323477
>1k	0.006619447130799828	5.059480041745227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5382036667313205E-4
2	0.0	0.0	0.0	0.0	2.5382036667313205E-4
3	0.0	0.0	0.0	0.0	2.5382036667313205E-4
4	0.0	0.0	0.0	0.0	2.5382036667313205E-4
5	0.0	0.0	0.0	0.0	2.96123761118654E-4
6	0.0	0.0	0.0	0.0	3.3842715556417605E-4
7	0.0	0.0	0.0	0.0	3.3842715556417605E-4
8	0.0	0.0	0.0	0.0	3.3842715556417605E-4
9	0.0	0.0	0.0	0.0	3.3842715556417605E-4
10	0.0	0.0	0.0	4.2303394445522006E-5	3.807305500096981E-4
11	0.0	0.0	0.0	4.2303394445522006E-5	3.807305500096981E-4
12	0.0	0.0	0.0	4.2303394445522006E-5	8.883712833559622E-4
13	0.0	0.0	0.0	4.2303394445522006E-5	0.0010787365583608112
14	0.0	0.0	0.0	4.2303394445522006E-5	0.0010787365583608112
15	0.0	6.345509166828301E-5	0.0	8.460678889104401E-5	0.0012056467416973772
16	0.0	6.345509166828301E-5	0.0	1.6921357778208802E-4	0.0012267984389201382
17	0.0	6.345509166828301E-5	0.0	1.9036527500484904E-4	0.0012267984389201382
18	0.0	6.345509166828301E-5	0.0	3.3842715556417605E-4	0.0013537086222567042
19	0.0	6.345509166828301E-5	0.0	4.2303394445522005E-4	0.0013537086222567042
20	0.0	6.345509166828301E-5	0.0	6.345509166828301E-4	0.0015017705028160311
21	0.0	6.345509166828301E-5	0.0	8.249161916876792E-4	0.0015652255944843142
22	0.0	6.345509166828301E-5	0.0	0.0010152814666925282	0.0016075289889298362
23	0.0	6.345509166828301E-5	0.0	0.001311405227811182	0.0016075289889298362
24	0.0	6.345509166828301E-5	0.0	0.0016075289889298362	0.0016075289889298362
25	0.0	6.345509166828301E-5	0.0	0.0018401976583802074	0.0016075289889298362
26	0.0	6.345509166828301E-5	0.0	0.002220928208389905	0.0016286806861525972
27	0.0	6.345509166828301E-5	0.0	0.0026862655472906474	0.0016498323833753583
28	0.0	6.345509166828301E-5	0.0	0.0030881477945231064	0.0016498323833753583
29	0.0	6.345509166828301E-5	0.0	0.003913063986210785	0.0016498323833753583
30	0.0	6.345509166828301E-5	0.0	0.005435986186249578	0.0016498323833753583
31	0.0	6.345509166828301E-5	0.0	0.009391353566905885	0.0016498323833753583
32	0.0	6.345509166828301E-5	0.0	0.014108182047581588	0.0017344391722664023
33	0.0	6.345509166828301E-5	0.0	0.018676948647697967	0.0017555908694891632
34	0.0	6.345509166828301E-5	0.0	0.024197541622838586	0.0018190459611574462
35	0.0	8.460678889104401E-5	0.0	0.030035410056320624	0.0018190459611574462
36	0.0	8.460678889104401E-5	0.0	0.038728757614875396	0.0018190459611574462
37	0.0	1.0575848611380501E-4	0.0	0.05038334278461671	0.0018190459611574462
38	0.0	1.0575848611380501E-4	0.0	0.06550680629889083	0.0019248044472712513
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2425	0.0	27.861181	1
GTATCAA	6220	0.0	27.56259	1
CGCAATA	1075	0.0	18.623394	36
TCTAGCG	1115	0.0	17.757229	28
ATACGAA	1150	0.0	17.601063	40
TCAACGC	9740	0.0	17.39159	4
ATCAACG	9860	0.0	17.179928	3
CAACGCA	9915	0.0	17.129005	5
TACGAAT	1180	0.0	16.967125	41
TATCAAC	10175	0.0	16.930035	2
AACGCAG	10090	0.0	16.853724	6
CTAGCGG	1175	0.0	16.66325	29
CAATACG	1205	0.0	16.249943	38
CGAATGC	1250	0.0	16.19332	43
TATACCG	290	0.0	15.930479	5
TAGCGGC	1275	0.0	15.701413	30
ACCGTCG	1290	0.0	15.689375	8
ATACCGT	1370	0.0	15.5761	6
AATACGA	1280	0.0	15.469683	39
GTCCTAT	1260	0.0	15.370396	1
>>END_MODULE