FastQCFastQC Report
Thu 2 Feb 2017
SRR4062168_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062168_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496527
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63530.18169457864904232No Hit
TATCAACGCAGAGTACTTTTTTTTT36890.10550469079746846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA400.00527731114.25143910
GGTATCA10950.013.9628491
GTATCAA32800.012.7970751
CCGTAAA951.3633651E-510.998592
GTATAAC1855.456968E-1210.7797781
CGTAAAC1002.4022227E-510.44940953
TAGACTG3250.010.2319495
GTTTAAG2450.010.07788851
TCTTATA2750.010.0168972
TAATACT3750.09.8804314
GTCTAGA3550.09.6302241
CCGTCTA1106.8315516E-59.5012329
CTAGGTT1901.0531949E-99.5004154
ACGTGCG800.00451887039.49973513
GTATTAG1606.681512E-89.4964711
GTCTTAT3400.09.496471
GTGTAGC5550.09.4109171
TAGGACT3750.09.3737434
GTATTAC2151.07320375E-109.2756231
AAGACGG2700.09.1492035
Produced by FastQC (version 0.11.5)