FastQCFastQC Report
Thu 2 Feb 2017
SRR4062168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496527
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38400.026.8786581
GGTATCA16100.026.7914471
TATCAAC56000.018.385472
CGCTATA600.007400739518.3393821
TCAACGC56250.018.1861654
ATCAACG56450.018.121733
CAACGCA58300.017.659895
AACGCAG60550.017.0399916
CTAATAC3250.016.922613
GTACCGT3400.016.8230656
TATGCGA1753.6343408E-916.34263812
ATACCGT2201.8189894E-1115.99955756
GTATTAC3500.015.7194721
TACCGTA2051.8153514E-915.0239757
ACGCAGA72350.014.3520577
AGAGTAC74800.013.852756511
TCGATCG1606.346596E-613.750841
AATACCG1302.3330332E-413.5380885
CGCAGAG77000.013.5139118
ACCGTAT2451.537046E-913.4690168
Produced by FastQC (version 0.11.5)