##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3496527
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29398285784723	32.0	32.0	32.0	32.0	32.0
2	31.462419137618557	32.0	32.0	32.0	32.0	32.0
3	31.576496906787792	32.0	32.0	32.0	32.0	32.0
4	31.650962226231915	32.0	32.0	32.0	32.0	32.0
5	31.622311510822023	32.0	32.0	32.0	32.0	32.0
6	35.26534301036428	36.0	36.0	36.0	36.0	36.0
7	35.27849177197831	36.0	36.0	36.0	36.0	36.0
8	35.22485311853734	36.0	36.0	36.0	36.0	36.0
9	35.31226528495275	36.0	36.0	36.0	36.0	36.0
10	35.19733838749136	36.0	36.0	36.0	36.0	36.0
11	35.31089707014989	36.0	36.0	36.0	36.0	36.0
12	35.24598637447959	36.0	36.0	36.0	36.0	36.0
13	35.26854676082868	36.0	36.0	36.0	36.0	36.0
14	35.236068819145395	36.0	36.0	36.0	36.0	36.0
15	35.210854656635	36.0	36.0	36.0	36.0	36.0
16	35.22935415628136	36.0	36.0	36.0	36.0	36.0
17	35.21052490085162	36.0	36.0	36.0	36.0	36.0
18	35.20045519454018	36.0	36.0	36.0	36.0	36.0
19	35.20064767124635	36.0	36.0	36.0	36.0	36.0
20	35.20003363337392	36.0	36.0	36.0	36.0	36.0
21	35.19272180652402	36.0	36.0	36.0	36.0	36.0
22	35.174200285025684	36.0	36.0	36.0	36.0	36.0
23	35.14263067323662	36.0	36.0	36.0	36.0	36.0
24	35.13562343433927	36.0	36.0	36.0	36.0	36.0
25	35.12353601159093	36.0	36.0	36.0	36.0	36.0
26	35.08164358519182	36.0	36.0	36.0	36.0	36.0
27	35.064509440367544	36.0	36.0	36.0	36.0	36.0
28	35.05460990291223	36.0	36.0	36.0	36.0	36.0
29	35.03523095917749	36.0	36.0	36.0	36.0	36.0
30	35.01767325120041	36.0	36.0	36.0	36.0	36.0
31	35.01414946888727	36.0	36.0	36.0	36.0	36.0
32	34.98863071842431	36.0	36.0	36.0	36.0	36.0
33	34.963559554952674	36.0	36.0	36.0	36.0	36.0
34	34.957327084847336	36.0	36.0	36.0	36.0	36.0
35	34.9484903162481	36.0	36.0	36.0	36.0	36.0
36	34.92247135514755	36.0	36.0	36.0	36.0	36.0
37	34.91639418199831	36.0	36.0	36.0	36.0	36.0
38	34.911384067676295	36.0	36.0	36.0	36.0	36.0
39	34.89430426248675	36.0	36.0	36.0	36.0	36.0
40	34.88275423012607	36.0	36.0	36.0	32.0	36.0
41	34.85668321737541	36.0	36.0	36.0	32.0	36.0
42	34.83834130266976	36.0	36.0	36.0	32.0	36.0
43	34.82543792740625	36.0	36.0	36.0	32.0	36.0
44	34.79803673759705	36.0	36.0	36.0	32.0	36.0
45	34.77561391632325	36.0	36.0	36.0	32.0	36.0
46	34.75804076444998	36.0	36.0	36.0	32.0	36.0
47	34.73117582103613	36.0	36.0	36.0	32.0	36.0
48	34.71047585218132	36.0	36.0	36.0	32.0	36.0
49	34.690657043403355	36.0	36.0	36.0	32.0	36.0
50	34.280281833945516	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	23.0
21	79.0
22	217.0
23	635.0
24	1573.0
25	3804.0
26	8035.0
27	15126.0
28	25893.0
29	41707.0
30	62726.0
31	92857.0
32	141798.0
33	239623.0
34	547855.0
35	2314570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.466292175012626	16.73036457863967	12.341030581577813	26.46231266476989
2	16.45080976725895	18.498244610489436	37.07056594329842	27.9803796789532
3	19.38513150217931	21.570246988434214	27.588945580176862	31.455675929209615
4	13.284557300589213	14.874278069146346	33.43347082218179	38.40769380808265
5	15.325778980113697	35.70236980867015	32.88019797930918	16.091653231906974
6	37.12335776047105	33.087517563887076	15.475639928946517	14.313484746695357
7	32.180875480155024	29.30044584240305	18.723436141062262	19.79524253637967
8	28.82300065178962	33.39439392288405	17.71025935163664	20.072346073689694
9	27.023758146297737	14.71957745500035	17.601179684870157	40.655484713831754
10	16.335356865643213	27.119575258413636	30.842441898043944	25.702625977899206
11	37.518743599005525	21.13551532706597	21.144038069776094	20.201703004152407
12	24.918962421557854	22.800917253296557	28.144993058824674	24.135127266320914
13	30.199051801973788	19.407343343838043	24.19217698018634	26.20142787400183
14	23.61454666301733	18.475704606313638	24.79117135374616	33.11857737692287
15	25.571660107300758	26.550545727231622	21.62946260675236	26.24833155871526
16	25.956340415260264	25.716672586862426	22.87903981860749	25.44794717926982
17	24.748500440580038	25.50548015216242	24.554221946520073	25.19179746073747
18	25.04262091226055	25.31517739194051	24.943865663117332	24.69833603268161
19	26.009666163024054	24.671280959649387	24.811563016673404	24.507489860653155
20	26.163972271843015	24.397614998692983	24.745446755886856	24.69296597357714
21	26.019740736948616	24.10472566198564	24.723168081690226	25.15236551937552
22	26.06120301659332	23.827758229809177	24.71681185359072	25.394226900006778
23	25.436933687894786	24.095602320703463	24.875290502630325	25.592173488771426
24	25.296653626967753	24.21005336190284	24.864421641972463	25.628871369156947
25	25.57771727317447	24.009586674750896	24.977606305698238	25.4350897463764
26	25.3117395984794	24.34380244518245	24.98881741440317	25.35564054193498
27	25.42199924438962	24.478458669227695	24.654891690602433	25.44465039578025
28	25.622842466830832	24.171063958792725	24.76823767971697	25.43785589465947
29	25.333697124760725	24.702782117820945	24.69377313200109	25.26974762541724
30	25.570686276416478	24.479335288054425	24.60237289260207	25.347605542927027
31	25.412810923788136	24.326729807614207	24.769170225447272	25.491289043150385
32	25.503102527553235	24.499814672026687	24.577520860836994	25.41956193958308
33	25.41506257219676	24.42922550729711	24.565360825803705	25.590351094702417
34	25.590246715004472	24.405148685658588	24.61864765491474	25.3859569444222
35	25.423592600642923	24.45359957900999	24.958615995332504	25.164191825014587
36	25.530784894695298	24.45480077334035	24.75301156578541	25.261402766178943
37	25.645642004060655	24.211943379643706	24.645522169289638	25.496892447006005
38	25.465755019194763	24.43567574338765	24.722331616486873	25.376237620930713
39	25.625717323031243	24.42417532697632	24.474339676711644	25.475767673280792
40	25.6773012283056	24.443304264463794	24.538341507165402	25.341053000065205
41	25.356364015964562	24.393342475822216	24.77220759646446	25.478085911748767
42	25.568862069932035	24.27526180039504	24.63309047164971	25.522785658023217
43	25.550553570968916	24.231021138946172	24.626314570170948	25.59211071991397
44	25.34277389807405	24.405267015997307	24.7649511597783	25.487007926150344
45	25.308661924922415	24.415444979683755	24.766313843446934	25.509579251946896
46	25.65728679059974	24.39975666796417	24.48984786599692	25.453108675439168
47	25.518984604850974	24.581158653061024	24.653059310033974	25.246797432054024
48	25.465987376133057	24.690817079201338	24.46172383939677	25.381471705268833
49	25.492507141639564	24.49419344948341	24.33648820099325	25.676811207883777
50	25.307166415556694	24.792705569836603	24.55715411745998	25.342973897146727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	90.0
1	95.0
2	100.0
3	295.5
4	491.0
5	542.5
6	594.0
7	478.5
8	363.0
9	488.5
10	614.0
11	1102.0
12	1590.0
13	2933.5
14	4277.0
15	5557.5
16	6838.0
17	6412.5
18	5987.0
19	5403.0
20	4819.0
21	5584.0
22	6349.0
23	8047.0
24	9745.0
25	12486.0
26	15227.0
27	18371.5
28	21516.0
29	25780.5
30	30045.0
31	36605.0
32	43165.0
33	50276.0
34	57387.0
35	67140.0
36	76893.0
37	87329.0
38	97765.0
39	110972.0
40	124179.0
41	139340.5
42	154502.0
43	168579.0
44	182656.0
45	200657.0
46	218658.0
47	231531.0
48	244404.0
49	259337.0
50	274270.0
51	285686.5
52	297103.0
53	300612.5
54	304122.0
55	297557.5
56	290993.0
57	279015.0
58	267037.0
59	248566.5
60	230096.0
61	202479.5
62	174863.0
63	151181.5
64	127500.0
65	107172.5
66	86845.0
67	70680.0
68	54515.0
69	43834.5
70	33154.0
71	25957.5
72	18761.0
73	15568.0
74	12375.0
75	9826.0
76	7277.0
77	5844.5
78	4412.0
79	3491.5
80	2571.0
81	1932.0
82	1293.0
83	938.0
84	583.0
85	419.5
86	256.0
87	179.5
88	103.0
89	71.0
90	39.0
91	33.0
92	27.0
93	28.0
94	29.0
95	24.0
96	19.0
97	21.5
98	24.0
99	15.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032088984297847546
2	0.004347170778318028
3	2.0019865426464602E-4
4	2.859980775209229E-5
5	0.0
6	1.7159884651255373E-4
7	0.0
8	0.0
9	0.0
10	2.859980775209229E-5
11	0.0
12	2.859980775209229E-5
13	0.0
14	0.0
15	0.0
16	6.005959627939381E-4
17	0.0
18	1.1439923100836916E-4
19	0.0
20	2.5739826976883064E-4
21	2.859980775209229E-5
22	0.0
23	2.859980775209229E-5
24	7.149951938023073E-4
25	2.0019865426464602E-4
26	5.433963472897535E-4
27	4.5759692403347663E-4
28	0.0016301890418692604
29	5.147965395376613E-4
30	0.0010009932713232303
31	3.145978852730152E-4
32	6.577955782981227E-4
33	1.7159884651255373E-4
34	6.577955782981227E-4
35	2.0019865426464602E-4
36	2.0019865426464602E-4
37	0.0013155911565962454
38	0.0
39	5.719961550418458E-4
40	8.579942325627687E-5
41	9.151938480669533E-4
42	0.0046045690480868585
43	0.003918173662036644
44	0.0036035757767636286
45	0.0018303876961339065
46	0.0020019865426464606
47	0.0010295930790753225
48	0.003832374238780367
49	0.00374657481552409
50	0.0014585901953567068
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3496527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.18422526188761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.0976812041089	26.617267974169017
2	16.76653500722726	14.145665792123985
3	7.27521782940254	9.206982832344533
4	3.8608461718898077	6.514672184659845
5	2.3472244882550624	4.950792327638521
6	1.4784695528061922	3.742085559505119
7	1.0133950519915083	2.9924499606744464
8	0.7191089790016624	2.4268044126441706
9	0.5325891654515051	2.0220175194702326
>10	2.72494029778287	20.275305849015034
>50	0.13801690780686876	3.934631989354857
>100	0.0456343133630248	2.975528440143223
>500	1.3641236657002212E-4	0.03366353242499674
>1k	2.0461854617606818E-4	0.16213162583203194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0019865426464602E-4
2	0.0	0.0	0.0	0.0	2.0019865426464602E-4
3	0.0	0.0	0.0	0.0	2.0019865426464602E-4
4	0.0	0.0	0.0	0.0	2.0019865426464602E-4
5	2.859980775209229E-5	0.0	0.0	0.0	2.2879846201673832E-4
6	2.859980775209229E-5	0.0	0.0	0.0	3.1459788527301517E-4
7	2.859980775209229E-5	0.0	0.0	0.0	3.1459788527301517E-4
8	2.859980775209229E-5	0.0	0.0	0.0	3.1459788527301517E-4
9	2.859980775209229E-5	0.0	0.0	0.0	3.1459788527301517E-4
10	2.859980775209229E-5	0.0	2.859980775209229E-5	0.0	3.1459788527301517E-4
11	2.859980775209229E-5	0.0	2.859980775209229E-5	0.0	3.1459788527301517E-4
12	2.859980775209229E-5	0.0	2.859980775209229E-5	0.0	0.0011153925023315993
13	2.859980775209229E-5	0.0	2.859980775209229E-5	0.0	0.0012869913488441531
14	2.859980775209229E-5	0.0	2.859980775209229E-5	0.0	0.0012869913488441531
15	2.859980775209229E-5	0.0	2.859980775209229E-5	8.579942325627687E-5	0.0017445882728776298
16	2.859980775209229E-5	0.0	2.859980775209229E-5	1.1439923100836916E-4	0.0018303876961339065
17	2.859980775209229E-5	0.0	2.859980775209229E-5	2.2879846201673832E-4	0.0018303876961339065
18	2.859980775209229E-5	0.0	2.859980775209229E-5	3.1459788527301517E-4	0.0019161871193901835
19	2.859980775209229E-5	0.0	2.859980775209229E-5	4.2899711628138434E-4	0.0019161871193901835
20	2.859980775209229E-5	0.0	2.859980775209229E-5	6.005959627939381E-4	0.0021163857736548296
21	2.859980775209229E-5	0.0	2.859980775209229E-5	7.721948093064919E-4	0.0022307850046631985
22	2.859980775209229E-5	0.0	2.859980775209229E-5	9.151938480669533E-4	0.0024023838511757524
23	2.859980775209229E-5	0.0	2.859980775209229E-5	0.0013727907721004298	0.0024023838511757524
24	2.859980775209229E-5	0.0	2.859980775209229E-5	0.001973386734894368	0.0024023838511757524
25	2.859980775209229E-5	0.0	2.859980775209229E-5	0.002659782120944583	0.0024023838511757524
26	2.859980775209229E-5	0.0	2.859980775209229E-5	0.003546376161259444	0.002545382889936214
27	2.859980775209229E-5	0.0	2.859980775209229E-5	0.0044901698170784895	0.002774181351952952
28	2.859980775209229E-5	0.0	2.859980775209229E-5	0.005462563280649628	0.0028027811597050446
29	2.859980775209229E-5	0.0	2.859980775209229E-5	0.007207151553527257	0.0028027811597050446
30	2.859980775209229E-5	0.0	2.859980775209229E-5	0.00986693367447184	0.0028027811597050446
31	2.859980775209229E-5	0.0	2.859980775209229E-5	0.01435710349155033	0.002859980775209229
32	2.859980775209229E-5	0.0	2.859980775209229E-5	0.02013426465747297	0.0030029798139696905
33	2.859980775209229E-5	0.0	2.859980775209229E-5	0.026226023708668632	0.003031579621721783
34	2.859980775209229E-5	0.0	2.859980775209229E-5	0.03223198333660801	0.0033175776992427058
35	2.859980775209229E-5	0.0	2.859980775209229E-5	0.03943913489013527	0.0033175776992427058
36	2.859980775209229E-5	0.0	2.859980775209229E-5	0.049334668372359204	0.0033175776992427058
37	2.859980775209229E-5	0.0	2.859980775209229E-5	0.0645211662887202	0.0033175776992427058
38	2.859980775209229E-5	0.0	2.859980775209229E-5	0.08248184555703417	0.003346177506994798
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3840	0.0	26.878658	1
GGTATCA	1610	0.0	26.791447	1
TATCAAC	5600	0.0	18.38547	2
CGCTATA	60	0.0074007395	18.339382	1
TCAACGC	5625	0.0	18.186165	4
ATCAACG	5645	0.0	18.12173	3
CAACGCA	5830	0.0	17.65989	5
AACGCAG	6055	0.0	17.039991	6
CTAATAC	325	0.0	16.92261	3
GTACCGT	340	0.0	16.823065	6
TATGCGA	175	3.6343408E-9	16.342638	12
ATACCGT	220	1.8189894E-11	15.9995575	6
GTATTAC	350	0.0	15.719472	1
TACCGTA	205	1.8153514E-9	15.023975	7
ACGCAGA	7235	0.0	14.352057	7
AGAGTAC	7480	0.0	13.8527565	11
TCGATCG	160	6.346596E-6	13.7508	41
AATACCG	130	2.3330332E-4	13.538088	5
CGCAGAG	7700	0.0	13.513911	8
ACCGTAT	245	1.537046E-9	13.469016	8
>>END_MODULE