FastQCFastQC Report
Thu 2 Feb 2017
SRR4062167_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062167_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1689222
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30440.18020129976995328No Hit
TATCAACGCAGAGTACTTTTTTTTT20520.12147604044939031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC307.716782E-419.0023126
GGTATCA7150.015.2729771
CGAGACT1155.316906E-912.3924454
GTATTAT1001.4510078E-712.344551
CGTCGTA2650.012.18980210
GTATCAA18600.012.1505491
GTTCAAA3150.012.0581681
CGAACGT951.0387812E-612.0011044
CGCGTAA1201.0000804E-811.87609310
CGCATCG2250.011.82331113
GCTCGTA1459.640644E-1111.79453859
CGTCTTA658.0218405E-411.69269115
AGGCCCG2700.011.61217910
GCATCGC2300.011.56593914
GCGTAAC1251.8284481E-811.40138711
GTTTTCG1501.7644197E-1011.40037315
ACCGTCG2850.011.3343768
ATCGCCA2450.011.244616
TAGACAG1701.0913936E-1111.17755
TCTACAC2550.011.1765053
Produced by FastQC (version 0.11.5)