##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062167_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1689222
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280295307543945	32.0	32.0	32.0	32.0	32.0
2	30.84051060192207	32.0	32.0	32.0	32.0	32.0
3	30.867363792325698	32.0	32.0	32.0	32.0	32.0
4	30.878200733828947	32.0	32.0	32.0	32.0	32.0
5	30.797638794664053	32.0	32.0	32.0	32.0	32.0
6	34.44604320805673	36.0	36.0	36.0	32.0	36.0
7	34.38885475088532	36.0	36.0	36.0	32.0	36.0
8	34.350021489182595	36.0	36.0	36.0	32.0	36.0
9	34.47143241089685	36.0	36.0	36.0	32.0	36.0
10	34.177977198970886	36.0	36.0	36.0	32.0	36.0
11	34.45708142565039	36.0	36.0	36.0	32.0	36.0
12	34.28711146314694	36.0	36.0	36.0	32.0	36.0
13	34.373709909058725	36.0	36.0	36.0	32.0	36.0
14	34.28578659288122	36.0	36.0	36.0	32.0	36.0
15	34.243997532591926	36.0	36.0	36.0	32.0	36.0
16	34.24147625356525	36.0	36.0	36.0	32.0	36.0
17	34.173272074363226	36.0	36.0	36.0	32.0	36.0
18	34.182053631790254	36.0	36.0	36.0	32.0	36.0
19	34.1851438117666	36.0	36.0	36.0	32.0	36.0
20	34.1696058895752	36.0	36.0	36.0	32.0	36.0
21	34.15257674834924	36.0	36.0	36.0	32.0	36.0
22	34.119569837475474	36.0	36.0	36.0	32.0	36.0
23	34.09261956095765	36.0	36.0	36.0	32.0	36.0
24	34.06696041136097	36.0	36.0	36.0	32.0	36.0
25	33.720534068346254	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	25.0
5	141.0
6	357.0
7	70.0
8	313.0
9	239.0
10	128.0
11	31.0
12	82.0
13	73.0
14	278.0
15	405.0
16	612.0
17	784.0
18	1061.0
19	1441.0
20	2119.0
21	2995.0
22	4508.0
23	6519.0
24	9439.0
25	13374.0
26	18356.0
27	23901.0
28	32135.0
29	42273.0
30	56296.0
31	77158.0
32	110185.0
33	156343.0
34	349250.0
35	778331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.143188659754145	17.318501898822227	11.798449566512058	25.73985987491157
2	16.87207741811739	19.109326984040536	37.32391989898278	26.694675698859292
3	19.053688378775654	22.68639027332879	28.248572854427305	30.011348493468255
4	13.043782011773642	15.401352376249758	34.79364154628671	36.76122406568989
5	15.138827860086057	36.18857327621432	33.13042819124051	15.542170672459108
6	35.09652065947294	34.60657198235213	16.38345488752091	13.913452470654022
7	30.924593257313603	29.936723976794642	20.02988083815692	19.108801927734834
8	28.315421532801295	33.12049365214639	18.810116739819193	19.753968075233118
9	26.963851336933896	14.431548225877577	18.508675015292113	40.09592542189641
10	16.572066755625162	26.66502265359123	30.743084030211108	26.019826560572497
11	37.16700102917254	21.264566111292915	21.792965582004182	19.775467277530364
12	24.399365931504477	23.52064516587981	28.49982571415699	23.58016318845872
13	29.411273055774878	19.36525042145921	25.051630235045014	26.171846287720896
14	23.574739212897107	19.37375533428165	24.833807491702228	32.217697961119015
15	25.281929927727298	27.08714693552364	21.821174858205133	25.809748278543925
16	25.867703837349758	25.901132913770763	23.22911838516223	25.002044863717245
17	24.28605129761436	26.078421144973372	24.794854590373046	24.84067296703922
18	24.899453677571383	25.14979712934767	25.555775501664808	24.394973691416137
19	25.692255180358487	25.01842932383937	24.997511152407544	24.2918043433946
20	25.731864271797715	24.44649468359951	24.71936610871719	25.102274935885582
21	26.499173397012378	24.24743575309754	24.37821086375568	24.875179986134402
22	26.040282058560514	24.068195165370728	24.812631596413762	25.078891179654995
23	24.734562561471282	24.154866155541598	25.254891040301462	25.855680242685658
24	24.80047734931292	24.855346448588662	25.0581725034708	25.286003698627617
25	25.0354631781407	24.461712210828445	24.97869839174339	25.524126219287464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	334.0
1	334.0
2	438.0
3	542.0
4	542.0
5	542.0
6	1170.0
7	1798.0
8	1798.0
9	1798.0
10	2248.0
11	2698.0
12	2698.0
13	2698.0
14	3608.0
15	4518.0
16	4518.0
17	4518.0
18	7500.0
19	10482.0
20	10482.0
21	10482.0
22	16359.5
23	22237.0
24	22237.0
25	22237.0
26	32710.5
27	43184.0
28	43184.0
29	43184.0
30	54698.0
31	66212.0
32	66212.0
33	66212.0
34	82111.0
35	98010.0
36	98010.0
37	98010.0
38	115841.0
39	133672.0
40	133672.0
41	133672.0
42	153904.5
43	174137.0
44	174137.0
45	174137.0
46	194468.0
47	214799.0
48	214799.0
49	214799.0
50	224284.5
51	233770.0
52	233770.0
53	233770.0
54	220797.5
55	207825.0
56	207825.0
57	207825.0
58	191385.0
59	174945.0
60	174945.0
61	174945.0
62	153304.5
63	131664.0
64	131664.0
65	131664.0
66	107190.0
67	82716.0
68	82716.0
69	82716.0
70	62853.5
71	42991.0
72	42991.0
73	42991.0
74	32264.0
75	21537.0
76	21537.0
77	21537.0
78	17010.0
79	12483.0
80	12483.0
81	12483.0
82	8718.0
83	4953.0
84	4953.0
85	4953.0
86	3549.5
87	2146.0
88	2146.0
89	2146.0
90	1453.5
91	761.0
92	761.0
93	761.0
94	481.0
95	201.0
96	201.0
97	201.0
98	404.0
99	607.0
100	607.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001006380452066099
2	2.959942506076762E-4
3	6.511873513368877E-4
4	0.011543775773699371
5	0.03457212847097658
6	0.06428995123198726
7	0.10969546927520481
8	0.11129383822848626
9	0.12301521055255024
10	0.1357429633286803
11	0.14385320579533065
12	0.1384069115841494
13	0.13177664037053743
14	0.11970007494574425
15	0.1348549805768573
16	0.12242322205133487
17	0.12585675535838392
18	0.11484576923577836
19	0.10028285210588068
20	0.09554694409615788
21	0.09424456939348409
22	0.10484116356523893
23	0.08536474187525382
24	0.09288299584068879
25	0.09211341078910883
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1689222.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.3063478491446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15490933536981	41.90001344635806
2	13.346420850453619	14.495907464916211
3	4.007238229911228	6.528554196838489
4	1.7438049434649434	3.7879871136346064
5	0.9171574736479344	2.49037363981837
6	0.594777680891223	1.9380122178831765
7	0.41143029076927395	1.5640293540333627
8	0.2940119116775988	1.277337051788453
9	0.2035979604509389	0.9950995495462566
>10	1.1783081787478635	11.49040774599401
>50	0.0788906896876811	2.926016832559458
>100	0.061346362793449655	6.784088702489796
>500	0.006024798208025587	2.2642377037513652
>1k	0.0020812939264088386	1.5579349803884321
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3044	0.18020129976995328	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2052	0.12147604044939031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.919885012153524E-5
2	0.0	0.0	0.0	0.0	5.919885012153524E-5
3	0.0	0.0	0.0	0.0	5.919885012153524E-5
4	0.0	0.0	0.0	0.0	5.919885012153524E-5
5	0.0	0.0	0.0	0.0	5.919885012153524E-5
6	0.0	0.0	0.0	0.0	5.919885012153524E-5
7	0.0	0.0	0.0	0.0	5.919885012153524E-5
8	0.0	0.0	0.0	0.0	5.919885012153524E-5
9	0.0	0.0	0.0	0.0	5.919885012153524E-5
10	0.0	0.0	0.0	0.0	5.919885012153524E-5
11	0.0	0.0	0.0	0.0	5.919885012153524E-5
12	0.0	0.0	0.0	0.0	2.959942506076762E-4
13	0.0	0.0	0.0	0.0	2.959942506076762E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGC	30	7.716782E-4	19.002312	6
GGTATCA	715	0.0	15.272977	1
CGAGACT	115	5.316906E-9	12.392445	4
GTATTAT	100	1.4510078E-7	12.34455	1
CGTCGTA	265	0.0	12.189802	10
GTATCAA	1860	0.0	12.150549	1
GTTCAAA	315	0.0	12.058168	1
CGAACGT	95	1.0387812E-6	12.001104	4
CGCGTAA	120	1.0000804E-8	11.876093	10
CGCATCG	225	0.0	11.823311	13
GCTCGTA	145	9.640644E-11	11.7945385	9
CGTCTTA	65	8.0218405E-4	11.692691	15
AGGCCCG	270	0.0	11.612179	10
GCATCGC	230	0.0	11.565939	14
GCGTAAC	125	1.8284481E-8	11.401387	11
GTTTTCG	150	1.7644197E-10	11.400373	15
ACCGTCG	285	0.0	11.334376	8
ATCGCCA	245	0.0	11.2446	16
TAGACAG	170	1.0913936E-11	11.1775	5
TCTACAC	255	0.0	11.176505	3
>>END_MODULE