FastQCFastQC Report
Thu 2 Feb 2017
SRR4062166_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062166_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698983
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15390.22017702862587502No Hit
TATCAACGCAGAGTACTTTTTTTTT10510.15036131064704006No Hit
GGTATCAACGCAGAGTACTTTTTTT7030.10057469208836266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC250.006029476418.9996413
GCGTTAT504.568312E-617.0947781
CGTTATT551.1282644E-515.5429342
CGTGGAA902.2755557E-914.7796179
GTATAGC456.77139E-414.7732651
ACTATCC400.00527089214.2517738
AACCGCG400.00527089214.2517737
GACGTGG1006.548362E-1014.2517737
GAACCGC400.00527089214.2517736
GGACCGA400.00527089214.2517736
TCGCGTA400.00527089214.2517739
TATTCCC801.2841156E-714.2507525
CTAAAAC400.005275906514.2497323
GTATTTA801.2897544E-714.2456481
AGGACGT1351.8189894E-1213.3710775
CTAAGGA500.001497507413.3007034
GGACGTG1301.4551915E-1113.1554836
TTGTACT604.0851877E-412.66733554
GTAGGAC2250.012.6664293
GCTCTAA751.4804638E-512.6627981
Produced by FastQC (version 0.11.5)