##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062166_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	698983
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24680857760489	32.0	32.0	32.0	32.0	32.0
2	30.809862328554484	32.0	32.0	32.0	32.0	32.0
3	30.841144920548853	32.0	32.0	32.0	32.0	32.0
4	30.842838810099817	32.0	32.0	32.0	32.0	32.0
5	30.767370594134622	32.0	32.0	32.0	32.0	32.0
6	34.43422515282918	36.0	36.0	36.0	32.0	36.0
7	34.354153105297264	36.0	36.0	36.0	32.0	36.0
8	34.31096178304766	36.0	36.0	36.0	32.0	36.0
9	34.42027774638296	36.0	36.0	36.0	32.0	36.0
10	34.13102893775671	36.0	36.0	36.0	32.0	36.0
11	34.42401317342482	36.0	36.0	36.0	32.0	36.0
12	34.25255406783856	36.0	36.0	36.0	32.0	36.0
13	34.336099733469915	36.0	36.0	36.0	32.0	36.0
14	34.2425595472279	36.0	36.0	36.0	32.0	36.0
15	34.20526250280765	36.0	36.0	36.0	32.0	36.0
16	34.19555411218871	36.0	36.0	36.0	32.0	36.0
17	34.1471881290389	36.0	36.0	36.0	32.0	36.0
18	34.14086894817184	36.0	36.0	36.0	32.0	36.0
19	34.13896475307697	36.0	36.0	36.0	32.0	36.0
20	34.11838199212284	36.0	36.0	36.0	32.0	36.0
21	34.09756317392554	36.0	36.0	36.0	32.0	36.0
22	34.06319753127043	36.0	36.0	36.0	32.0	36.0
23	34.0395059679563	36.0	36.0	36.0	32.0	36.0
24	34.02119078718653	36.0	36.0	36.0	32.0	36.0
25	33.6707945686805	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	11.0
5	43.0
6	148.0
7	37.0
8	129.0
9	92.0
10	54.0
11	9.0
12	33.0
13	30.0
14	141.0
15	181.0
16	289.0
17	382.0
18	552.0
19	722.0
20	1021.0
21	1480.0
22	2185.0
23	3151.0
24	4316.0
25	5853.0
26	7927.0
27	10213.0
28	13303.0
29	17742.0
30	23080.0
31	31470.0
32	44962.0
33	64123.0
34	142458.0
35	322845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27395712025271	17.642758427303864	11.801229795501431	25.282054656941995
2	16.603551159327193	19.4153505879256	37.8591467889777	26.121951463769506
3	19.307364138132314	23.078133628619906	28.19843715574936	29.41606507749842
4	12.918899893262978	15.535816996082493	35.18358481873307	36.36169829192147
5	14.977367082593332	36.27577955838978	33.42387454365656	15.32297881536033
6	34.56398128689796	34.88206966450409	16.600147735016062	13.953801313581888
7	30.668683127748825	30.326829959914242	20.019964110429886	18.984522801907044
8	28.118845914224128	33.26296341384817	18.918671182945083	19.69951948898262
9	27.042260380320503	14.755610558219749	18.616396523373268	39.58573253808648
10	16.609747013150734	26.880067615964244	30.95249692003553	25.557688450849497
11	36.91256129935287	21.624219745017616	21.794561388176945	19.66865756745257
12	24.491047215747848	23.87375707505612	28.522128039355295	23.113067669840742
13	29.556276250121762	20.100072769778198	24.77624725680856	25.567403723291488
14	23.333462241950016	19.902201329477535	25.028037624128256	31.736298804444196
15	25.063856182839984	27.434879586507044	22.217510704774565	25.283753525878407
16	25.324794380448452	26.242669745349744	23.752889846361875	24.679646027839926
17	23.69012865946067	26.487490725116814	25.089597520204205	24.732783095218313
18	24.296937102098983	25.808119508160328	26.08612207191401	23.80882131782668
19	25.4739380608279	25.209008188481135	25.32586374298652	23.991190007704446
20	25.55221264536528	24.779222341395936	25.188486196294857	24.480078816943923
21	26.320545316549953	24.503587232031617	24.80989818275551	24.365969268662916
22	25.648652712834135	24.60691294727571	25.23791451547071	24.506519824419446
23	24.428474471430167	24.87650237544996	25.582837195050356	25.112185958069517
24	24.611828925493207	25.044570044061693	25.44466226288379	24.89893876756131
25	24.587849341804745	24.95628976342774	25.410503917095895	25.045356977671624
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	689.0
1	689.0
2	505.0
3	321.0
4	321.0
5	321.0
6	611.0
7	901.0
8	901.0
9	901.0
10	1094.5
11	1288.0
12	1288.0
13	1288.0
14	1629.5
15	1971.0
16	1971.0
17	1971.0
18	3325.5
19	4680.0
20	4680.0
21	4680.0
22	7327.5
23	9975.0
24	9975.0
25	9975.0
26	14869.0
27	19763.0
28	19763.0
29	19763.0
30	25013.5
31	30264.0
32	30264.0
33	30264.0
34	36801.0
35	43338.0
36	43338.0
37	43338.0
38	50801.5
39	58265.0
40	58265.0
41	58265.0
42	66052.5
43	73840.0
44	73840.0
45	73840.0
46	81959.0
47	90078.0
48	90078.0
49	90078.0
50	92626.0
51	95174.0
52	95174.0
53	95174.0
54	88757.5
55	82341.0
56	82341.0
57	82341.0
58	75383.5
59	68426.0
60	68426.0
61	68426.0
62	59705.0
63	50984.0
64	50984.0
65	50984.0
66	41573.0
67	32162.0
68	32162.0
69	32162.0
70	24650.5
71	17139.0
72	17139.0
73	17139.0
74	13083.5
75	9028.0
76	9028.0
77	9028.0
78	6971.5
79	4915.0
80	4915.0
81	4915.0
82	3384.0
83	1853.0
84	1853.0
85	1853.0
86	1380.0
87	907.0
88	907.0
89	907.0
90	604.5
91	302.0
92	302.0
93	302.0
94	203.0
95	104.0
96	104.0
97	104.0
98	189.5
99	275.0
100	275.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.153249792913418E-4
2	0.0
3	1.430649958582684E-4
4	0.009871484714220518
5	0.03176042908053558
6	0.06223327319834674
7	0.1044374469765359
8	0.10987391681915011
9	0.11945927154165409
10	0.13204899117718172
11	0.13934530596595338
12	0.13333657613990613
13	0.1274709113097171
14	0.11645490662863045
15	0.1330504461481896
16	0.12146218148366984
17	0.12317896143396907
18	0.11273521673631547
19	0.09799952216291384
20	0.09385063728302405
21	0.09628274221261461
22	0.10415131698481936
23	0.08426528256052007
24	0.09213385733272482
25	0.09070320737414216
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	698983.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.39668494603636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.36655020566712	47.50049708339048
2	14.185504748454544	17.41885931682733
3	4.200997848936118	7.737820241703218
4	1.5906683938485437	3.906470649229468
5	0.7618378951082303	2.338716061295576
6	0.45331524640997145	1.6699232019040715
7	0.2882419690139584	1.2387970951842624
8	0.20058976567527464	0.9852437317251276
9	0.14146283105383403	0.7816813982807955
>10	0.6989999162689043	7.5678054011052405
>50	0.06280314636860393	2.6529722003900456
>100	0.04692683177259487	5.270644555785602
>500	0.0016342677729185837	0.559316155964828
>1k	4.669336494053097E-4	0.3712529072139683
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1539	0.22017702862587502	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1051	0.15036131064704006	No Hit
GGTATCAACGCAGAGTACTTTTTTT	703	0.10057469208836266	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4306499585826836E-4	0.0
11	0.0	0.0	0.0	1.4306499585826836E-4	0.0
12	0.0	0.0	0.0	1.4306499585826836E-4	1.4306499585826836E-4
13	0.0	0.0	0.0	1.4306499585826836E-4	2.8612999171653673E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTATAC	25	0.0060294764	18.999641	3
GCGTTAT	50	4.568312E-6	17.094778	1
CGTTATT	55	1.1282644E-5	15.542934	2
CGTGGAA	90	2.2755557E-9	14.779617	9
GTATAGC	45	6.77139E-4	14.773265	1
ACTATCC	40	0.005270892	14.251773	8
AACCGCG	40	0.005270892	14.251773	7
GACGTGG	100	6.548362E-10	14.251773	7
GAACCGC	40	0.005270892	14.251773	6
GGACCGA	40	0.005270892	14.251773	6
TCGCGTA	40	0.005270892	14.251773	9
TATTCCC	80	1.2841156E-7	14.250752	5
CTAAAAC	40	0.0052759065	14.249732	3
GTATTTA	80	1.2897544E-7	14.245648	1
AGGACGT	135	1.8189894E-12	13.371077	5
CTAAGGA	50	0.0014975074	13.300703	4
GGACGTG	130	1.4551915E-11	13.155483	6
TTGTACT	60	4.0851877E-4	12.6673355	4
GTAGGAC	225	0.0	12.666429	3
GCTCTAA	75	1.4804638E-5	12.662798	1
>>END_MODULE