FastQCFastQC Report
Thu 2 Feb 2017
SRR4062166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698983
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.11016004681086665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC2450.024.2442283
AACCGTA551.5929797E-423.9993367
CGTCGTA1700.023.29347410
GTATCAA10750.023.1315611
GTCCTAC2000.023.1059741
ATACCGT1750.022.6279456
TACCGTC1750.022.6279457
CTTAACG500.00257870122.00096741
ACCGTCG1950.021.4353058
TGTAGGA2700.021.18462
TATATCA1057.7367076E-820.9518014
GTCGTAG1800.020.77720311
GATTTCG856.105034E-620.7067941
TTAGACG550.004482470519.9994474
CGAAAGC1452.9467628E-1019.72359319
GGTATCA5350.019.33211
CCGTCGT2100.018.856629
ATTTCGT951.5949356E-518.5271342
ACCGTAA600.007410235718.3328278
GCGAAAG1408.3597115E-817.28523618
Produced by FastQC (version 0.11.5)