##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	698983
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2865076833056	32.0	32.0	32.0	32.0	32.0
2	31.480655466585024	32.0	32.0	32.0	32.0	32.0
3	31.592028704560768	32.0	32.0	32.0	32.0	32.0
4	31.66487453915188	32.0	32.0	32.0	32.0	32.0
5	31.637683606039058	32.0	32.0	32.0	32.0	32.0
6	35.28812288710884	36.0	36.0	36.0	36.0	36.0
7	35.29341343065568	36.0	36.0	36.0	36.0	36.0
8	35.256930426061864	36.0	36.0	36.0	36.0	36.0
9	35.323522889684014	36.0	36.0	36.0	36.0	36.0
10	35.2181555202344	36.0	36.0	36.0	36.0	36.0
11	35.32587058626605	36.0	36.0	36.0	36.0	36.0
12	35.262531420649715	36.0	36.0	36.0	36.0	36.0
13	35.28146607285156	36.0	36.0	36.0	36.0	36.0
14	35.24640513431657	36.0	36.0	36.0	36.0	36.0
15	35.2378341104147	36.0	36.0	36.0	36.0	36.0
16	35.24950678342678	36.0	36.0	36.0	36.0	36.0
17	35.22894262092211	36.0	36.0	36.0	36.0	36.0
18	35.22765360530943	36.0	36.0	36.0	36.0	36.0
19	35.216958066219064	36.0	36.0	36.0	36.0	36.0
20	35.209676057929876	36.0	36.0	36.0	36.0	36.0
21	35.19746689118333	36.0	36.0	36.0	36.0	36.0
22	35.18113602190611	36.0	36.0	36.0	36.0	36.0
23	35.15232845434009	36.0	36.0	36.0	36.0	36.0
24	35.14219945263333	36.0	36.0	36.0	36.0	36.0
25	35.125030222480376	36.0	36.0	36.0	36.0	36.0
26	35.088099424449524	36.0	36.0	36.0	36.0	36.0
27	35.073844428262205	36.0	36.0	36.0	36.0	36.0
28	35.0530742521635	36.0	36.0	36.0	36.0	36.0
29	35.03163596253414	36.0	36.0	36.0	36.0	36.0
30	35.00479983061104	36.0	36.0	36.0	36.0	36.0
31	35.014119084441255	36.0	36.0	36.0	36.0	36.0
32	34.98166765143072	36.0	36.0	36.0	36.0	36.0
33	34.96709934290247	36.0	36.0	36.0	36.0	36.0
34	34.93688401577721	36.0	36.0	36.0	36.0	36.0
35	34.932261585761026	36.0	36.0	36.0	36.0	36.0
36	34.9107117054349	36.0	36.0	36.0	36.0	36.0
37	34.89457826585196	36.0	36.0	36.0	36.0	36.0
38	34.873848147952096	36.0	36.0	36.0	32.0	36.0
39	34.85389773427966	36.0	36.0	36.0	32.0	36.0
40	34.853687428735746	36.0	36.0	36.0	32.0	36.0
41	34.81664217870821	36.0	36.0	36.0	32.0	36.0
42	34.787147899162065	36.0	36.0	36.0	32.0	36.0
43	34.78215063885674	36.0	36.0	36.0	32.0	36.0
44	34.728322434165065	36.0	36.0	36.0	32.0	36.0
45	34.70870393128302	36.0	36.0	36.0	32.0	36.0
46	34.67411939918424	36.0	36.0	36.0	32.0	36.0
47	34.6487668512682	36.0	36.0	36.0	32.0	36.0
48	34.61009638288771	36.0	36.0	36.0	32.0	36.0
49	34.600675552910445	36.0	36.0	36.0	32.0	36.0
50	34.1945526572177	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	20.0
22	49.0
23	128.0
24	373.0
25	858.0
26	1679.0
27	3151.0
28	5442.0
29	8519.0
30	12720.0
31	18660.0
32	28402.0
33	47876.0
34	108980.0
35	462114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81920522313832	18.15175094491919	12.271283759125394	25.7577600728171
2	16.13787045064998	19.859474122464395	37.477859519905685	26.524795906979936
3	18.756133920472916	23.450389280410654	28.460389537928016	29.33308726118841
4	12.630922354334798	15.911116579373175	35.26595067405073	36.1920103922413
5	14.518235779697076	36.676571533213256	33.19336807905199	15.611824608037677
6	34.75020136398167	35.154646107272995	16.337307202034957	13.757845326710378
7	30.669844617107998	30.203595795605903	20.042976724755825	19.083582862530278
8	28.062342002595198	33.37191319388311	18.89645384794766	19.669290955574027
9	26.918108165720767	14.403926848006318	18.67456003937149	40.003404946901426
10	16.508556002077306	26.932271600310738	30.892453750663464	25.666718646948496
11	37.09303373615667	21.396371585575043	21.844021957615563	19.66657272065272
12	24.46411429176389	23.74864624747669	28.668508390046682	23.118731070712737
13	29.587271793448483	19.838966040661933	24.914339833729862	25.659422332159725
14	23.33061032957883	19.681165350230263	25.144388347069956	31.84383597312095
15	25.089308323664522	27.364614017794427	22.199824602315076	25.346253056225976
16	25.502589487538984	26.080717617099204	23.813413831583162	24.60327906377865
17	23.84879174457748	26.33683508754863	25.1901691457446	24.62420402212929
18	24.48661052788198	25.4734742811689	26.23729938682255	23.802615804126575
19	25.489604182075958	25.025072140524163	25.60119487884541	23.88412879855447
20	25.631095462979786	24.434929032608803	25.548833090361285	24.385142414050126
21	26.271025189453823	24.295154531655278	25.02120938563599	24.412610893254914
22	25.70734910577224	24.458105561937842	25.43681319860426	24.397732133685654
23	24.392581793834758	24.65510606123468	25.844119241812745	25.108192903117814
24	24.753711847869315	24.843700373974574	25.579345844933606	24.823241933222505
25	24.807827394449923	24.527848396451404	25.615431606867713	25.04889260223096
26	24.36106898623709	25.56010186271424	25.861111905920055	24.21771724512862
27	24.809865804457925	25.132192623537154	25.396291739391685	24.661649832613236
28	24.520441331318956	24.993132811427003	25.739653435883348	24.746772421370693
29	24.413504210408853	25.24256704750623	25.680060430740703	24.66386831134421
30	24.339744653576968	25.22175423335985	25.888161471418027	24.55033964164516
31	25.04438616896931	24.808470640748865	25.192316221231252	24.954826969050572
32	24.6683386243689	25.035015257925465	25.15032597452577	25.146320143179857
33	24.514187040314287	24.72263273927978	25.642254532656732	25.120925687749203
34	25.022068028184126	24.961693908938088	25.640258950606242	24.37597911227154
35	25.230370409580775	24.697453300008725	25.694473256145002	24.377703034265497
36	24.36875966585644	25.214991537681282	25.52200989726473	24.894238899197546
37	25.192676642311746	24.92871643806807	25.272508508961057	24.60609841065913
38	24.602601207754695	25.080152163929593	25.523796716086082	24.793449912229626
39	25.289278663194942	24.71086440241495	25.21030644653638	24.789550487853727
40	25.325687929016773	24.90307332663788	25.474618802773175	24.296619941572175
41	24.431949869093096	25.17418487202598	25.939024564716657	24.45484069416427
42	25.33922214973274	25.219757579919648	25.436939875699622	24.004080394647985
43	24.964089298897214	24.58309249795409	25.465694550157664	24.987123652991034
44	24.379433385124713	25.31883174668578	25.32813130402685	24.97360356416265
45	24.676236966499182	25.309456226894504	25.239782078721774	24.77452472788454
46	24.54954890509696	25.251728982525616	25.21481731586372	24.983904796513706
47	24.753281237928736	25.08033202951755	25.581495162910205	24.584891569643506
48	25.099826742836868	25.538477826373718	24.807106548147896	24.55458888264152
49	24.92063196839872	25.519379534421905	24.878855555189933	24.681132941989446
50	24.895560311773927	25.81191127490815	24.699413993201404	24.593114420116517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	305.0
1	171.0
2	37.0
3	116.0
4	195.0
5	199.5
6	204.0
7	143.5
8	83.0
9	140.5
10	198.0
11	336.5
12	475.0
13	874.5
14	1274.0
15	1617.0
16	1960.0
17	1956.0
18	1952.0
19	1854.0
20	1756.0
21	2023.0
22	2290.0
23	2669.0
24	3048.0
25	3525.0
26	4002.0
27	5216.5
28	6431.0
29	7678.0
30	8925.0
31	10697.0
32	12469.0
33	14587.5
34	16706.0
35	18876.5
36	21047.0
37	23755.0
38	26463.0
39	28607.5
40	30752.0
41	33291.0
42	35830.0
43	37529.5
44	39229.0
45	42036.0
46	44843.0
47	47332.0
48	49821.0
49	51457.0
50	53093.0
51	52382.0
52	51671.0
53	51002.5
54	50334.0
55	50432.5
56	50531.0
57	48297.0
58	46063.0
59	42367.5
60	38672.0
61	34115.0
62	29558.0
63	25522.5
64	21487.0
65	18286.5
66	15086.0
67	12891.5
68	10697.0
69	9426.0
70	8155.0
71	6419.0
72	4683.0
73	4075.0
74	3467.0
75	2617.0
76	1767.0
77	1565.5
78	1364.0
79	1148.5
80	933.0
81	732.0
82	531.0
83	428.0
84	325.0
85	246.0
86	167.0
87	111.0
88	55.0
89	37.0
90	19.0
91	14.0
92	9.0
93	7.5
94	6.0
95	6.5
96	7.0
97	7.5
98	8.0
99	4.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03519398898113402
2	0.00529340484675593
3	1.430649958582684E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.291949875748051E-4
17	0.0
18	1.430649958582684E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	7.153249792913418E-4
25	2.861299917165368E-4
26	4.291949875748051E-4
27	4.291949875748051E-4
28	0.0010014549710078786
29	1.430649958582684E-4
30	0.0015737149544409522
31	5.722599834330736E-4
32	2.861299917165368E-4
33	0.0
34	0.0011445199668661471
35	0.0
36	2.861299917165368E-4
37	0.0020029099420157573
38	0.0
39	4.291949875748051E-4
40	1.430649958582684E-4
41	0.001859844946157489
42	0.004435014871606319
43	0.003862754888173246
44	0.0035766248964567092
45	0.0021459749378740255
46	0.0024321049295905625
47	0.0012875849627244154
48	0.0031474299088819044
49	0.003433559900598441
50	0.0021459749378740255
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	698983.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.31530537914899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30804150188655	48.94782255955871
2	14.371490825303521	18.198706607154605
3	4.276552187232967	8.123136231135685
4	1.632700055696001	4.134996104757835
5	0.7320496724993152	2.3174974283500074
6	0.42280618850969426	1.6062061765011195
7	0.24520897449654822	1.0867836771369837
8	0.19284184317420217	0.9767872152362056
9	0.1200312233439328	0.6839832204948548
>10	0.6108275592268211	6.887032897603665
>50	0.05065302236435344	2.2971790955123157
>100	0.035206954761125064	4.1571286833646495
>500	0.0015899915049929665	0.5827401031933592
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	770	0.11016004681086665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.8612999171653673E-4	0.0	0.0	0.0
6	0.0	2.8612999171653673E-4	0.0	0.0	0.0
7	0.0	2.8612999171653673E-4	0.0	0.0	0.0
8	0.0	2.8612999171653673E-4	0.0	0.0	0.0
9	1.4306499585826836E-4	2.8612999171653673E-4	0.0	0.0	0.0
10	1.4306499585826836E-4	2.8612999171653673E-4	0.0	1.4306499585826836E-4	0.0
11	1.4306499585826836E-4	2.8612999171653673E-4	0.0	1.4306499585826836E-4	0.0
12	1.4306499585826836E-4	2.8612999171653673E-4	0.0	1.4306499585826836E-4	1.4306499585826836E-4
13	1.4306499585826836E-4	2.8612999171653673E-4	0.0	1.4306499585826836E-4	4.291949875748051E-4
14	1.4306499585826836E-4	2.8612999171653673E-4	0.0	1.4306499585826836E-4	4.291949875748051E-4
15	1.4306499585826836E-4	2.8612999171653673E-4	0.0	1.4306499585826836E-4	5.722599834330735E-4
16	1.4306499585826836E-4	2.8612999171653673E-4	0.0	1.4306499585826836E-4	5.722599834330735E-4
17	1.4306499585826836E-4	2.8612999171653673E-4	0.0	1.4306499585826836E-4	5.722599834330735E-4
18	1.4306499585826836E-4	2.8612999171653673E-4	0.0	2.8612999171653673E-4	5.722599834330735E-4
19	1.4306499585826836E-4	2.8612999171653673E-4	0.0	2.8612999171653673E-4	5.722599834330735E-4
20	1.4306499585826836E-4	2.8612999171653673E-4	0.0	4.291949875748051E-4	7.153249792913418E-4
21	1.4306499585826836E-4	2.8612999171653673E-4	0.0	8.583899751496102E-4	7.153249792913418E-4
22	1.4306499585826836E-4	2.8612999171653673E-4	0.0	0.001144519966866147	7.153249792913418E-4
23	1.4306499585826836E-4	2.8612999171653673E-4	0.0	0.001573714954440952	7.153249792913418E-4
24	1.4306499585826836E-4	2.8612999171653673E-4	0.0	0.0018598449461574888	8.583899751496102E-4
25	1.4306499585826836E-4	2.8612999171653673E-4	0.0	0.002289039933732294	8.583899751496102E-4
26	1.4306499585826836E-4	2.8612999171653673E-4	0.0	0.002575169925448831	8.583899751496102E-4
27	1.4306499585826836E-4	2.8612999171653673E-4	0.0	0.0032904949047401727	8.583899751496102E-4
28	1.4306499585826836E-4	2.8612999171653673E-4	0.0	0.004578079867464588	8.583899751496102E-4
29	1.4306499585826836E-4	2.8612999171653673E-4	0.0	0.006008729826047271	8.583899751496102E-4
30	1.4306499585826836E-4	4.291949875748051E-4	0.0	0.01044374469765359	8.583899751496102E-4
31	1.4306499585826836E-4	4.291949875748051E-4	0.0	0.017740059486425278	8.583899751496102E-4
32	1.4306499585826836E-4	4.291949875748051E-4	0.0	0.026180894242063112	8.583899751496102E-4
33	1.4306499585826836E-4	4.291949875748051E-4	0.0	0.03333414403497653	8.583899751496102E-4
34	1.4306499585826836E-4	4.291949875748051E-4	0.0	0.041631913794756094	8.583899751496102E-4
35	1.4306499585826836E-4	4.291949875748051E-4	0.0	0.052361788484126225	8.583899751496102E-4
36	1.4306499585826836E-4	4.291949875748051E-4	0.0	0.06409311814450423	8.583899751496102E-4
37	1.4306499585826836E-4	7.153249792913418E-4	0.0	0.08154704763921297	8.583899751496102E-4
38	1.4306499585826836E-4	7.153249792913418E-4	0.0	0.10257760203037843	0.0010014549710078786
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGAC	245	0.0	24.244228	3
AACCGTA	55	1.5929797E-4	23.999336	7
CGTCGTA	170	0.0	23.293474	10
GTATCAA	1075	0.0	23.131561	1
GTCCTAC	200	0.0	23.105974	1
ATACCGT	175	0.0	22.627945	6
TACCGTC	175	0.0	22.627945	7
CTTAACG	50	0.002578701	22.000967	41
ACCGTCG	195	0.0	21.435305	8
TGTAGGA	270	0.0	21.1846	2
TATATCA	105	7.7367076E-8	20.951801	4
GTCGTAG	180	0.0	20.777203	11
GATTTCG	85	6.105034E-6	20.70679	41
TTAGACG	55	0.0044824705	19.999447	4
CGAAAGC	145	2.9467628E-10	19.723593	19
GGTATCA	535	0.0	19.3321	1
CCGTCGT	210	0.0	18.85662	9
ATTTCGT	95	1.5949356E-5	18.52713	42
ACCGTAA	60	0.0074102357	18.332827	8
GCGAAAG	140	8.3597115E-8	17.285236	18
>>END_MODULE