FastQCFastQC Report
Thu 2 Feb 2017
SRR4062165_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062165_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320374
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47470.2045790894054148No Hit
TATCAACGCAGAGTACTTTTTTTTT28830.12424721187187927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAT602.566173E-514.25186110
AATGCGA551.9586357E-413.81998610
GGTATCA9700.013.8048261
GTATCAA25250.012.6375081
GTTCTAG1501.7826096E-1011.3963241
GTCTAGG1451.2241799E-911.134341
GTATAAT2000.010.9214781
TCGAAAT700.001490793710.85809213
TAATACT2200.010.7945354
GTATTAG1158.858424E-710.7356691
CCCTATA1451.4486432E-810.4807362
TAACCGT750.002646965210.1346
GTCCTAA1502.458546E-810.1300661
GACGCTC1606.166374E-910.0946338
CCTATAC1151.0286669E-59.9115573
ATAGGAC1252.5759655E-69.8785193
ACGCTCT1752.570232E-99.7724949
TGGATTG1951.6370905E-109.743395
GTATAAG2151.0913936E-119.7177961
AACCGAG800.00451360469.5008317
Produced by FastQC (version 0.11.5)