FastQCFastQC Report
Thu 2 Feb 2017
SRR4062165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320374
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24600.025.857421
GGTATCA12600.025.1544251
GTATAGG3200.017.8832231
TATCAAC37550.016.8732622
TCAACGC37300.016.750074
CAACGCA37700.016.5723515
GTATTAA4000.016.5075911
ATCAACG38100.016.3983633
AACGCAG38650.016.165016
AATATAG1805.2750693E-915.8883225
TAGACAG5250.015.5042075
TATGCCG1901.078115E-815.0520945
GTATTAC2509.094947E-1214.9668841
GTCTTAT2800.014.935441
TTATACC2102.5484042E-914.6661424
TAATACA4100.014.4872874
GTATCGA1402.7597214E-514.1423519
TAGGACG1251.6725744E-414.0794964
CTTACAC4900.013.917873
TCGCGTG950.006238248713.89603744
Produced by FastQC (version 0.11.5)