##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062165_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2320374
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297333964266105	32.0	32.0	32.0	32.0	32.0
2	31.482168822784605	32.0	32.0	32.0	32.0	32.0
3	31.584192031112227	32.0	32.0	32.0	32.0	32.0
4	31.66225099919237	32.0	32.0	32.0	32.0	32.0
5	31.638517325224296	32.0	32.0	32.0	32.0	32.0
6	35.29070917015964	36.0	36.0	36.0	36.0	36.0
7	35.292154195832225	36.0	36.0	36.0	36.0	36.0
8	35.24915509310137	36.0	36.0	36.0	36.0	36.0
9	35.32676585757296	36.0	36.0	36.0	36.0	36.0
10	35.21652026785337	36.0	36.0	36.0	36.0	36.0
11	35.330373896621836	36.0	36.0	36.0	36.0	36.0
12	35.260902768260635	36.0	36.0	36.0	36.0	36.0
13	35.28501224371588	36.0	36.0	36.0	36.0	36.0
14	35.255790230368035	36.0	36.0	36.0	36.0	36.0
15	35.24048580099588	36.0	36.0	36.0	36.0	36.0
16	35.250240693957096	36.0	36.0	36.0	36.0	36.0
17	35.236210197149255	36.0	36.0	36.0	36.0	36.0
18	35.22806926814384	36.0	36.0	36.0	36.0	36.0
19	35.22266151922061	36.0	36.0	36.0	36.0	36.0
20	35.22229907764869	36.0	36.0	36.0	36.0	36.0
21	35.21493216179805	36.0	36.0	36.0	36.0	36.0
22	35.19838181258711	36.0	36.0	36.0	36.0	36.0
23	35.17427621581693	36.0	36.0	36.0	36.0	36.0
24	35.16020865601838	36.0	36.0	36.0	36.0	36.0
25	35.151165717250755	36.0	36.0	36.0	36.0	36.0
26	35.10818557698026	36.0	36.0	36.0	36.0	36.0
27	35.095967288032014	36.0	36.0	36.0	36.0	36.0
28	35.08124767817602	36.0	36.0	36.0	36.0	36.0
29	35.06550323353046	36.0	36.0	36.0	36.0	36.0
30	35.04454669807539	36.0	36.0	36.0	36.0	36.0
31	35.04340550273361	36.0	36.0	36.0	36.0	36.0
32	35.022579549676045	36.0	36.0	36.0	36.0	36.0
33	35.00154328569446	36.0	36.0	36.0	36.0	36.0
34	34.994407367088236	36.0	36.0	36.0	36.0	36.0
35	34.98574798717793	36.0	36.0	36.0	36.0	36.0
36	34.965530556711975	36.0	36.0	36.0	36.0	36.0
37	34.94925947282636	36.0	36.0	36.0	36.0	36.0
38	34.94710637164526	36.0	36.0	36.0	36.0	36.0
39	34.92707468709786	36.0	36.0	36.0	36.0	36.0
40	34.921481192256074	36.0	36.0	36.0	36.0	36.0
41	34.893623183159264	36.0	36.0	36.0	36.0	36.0
42	34.87537396988589	36.0	36.0	36.0	36.0	36.0
43	34.85458206306397	36.0	36.0	36.0	32.0	36.0
44	34.82794368494044	36.0	36.0	36.0	32.0	36.0
45	34.81123689543151	36.0	36.0	36.0	32.0	36.0
46	34.79299888724835	36.0	36.0	36.0	32.0	36.0
47	34.766231650587365	36.0	36.0	36.0	32.0	36.0
48	34.74797855862891	36.0	36.0	36.0	32.0	36.0
49	34.72910013644352	36.0	36.0	36.0	32.0	36.0
50	34.32779500201261	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	16.0
21	59.0
22	161.0
23	458.0
24	1018.0
25	2362.0
26	5048.0
27	9563.0
28	16921.0
29	27014.0
30	40583.0
31	59505.0
32	90769.0
33	152826.0
34	352554.0
35	1561513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5310536446781	16.989500412784018	12.059199479223922	26.42024646331396
2	16.37261413184719	19.173400417277765	37.04777489929004	27.406210551585
3	18.97303314518781	22.226527096791155	28.205107209333697	30.59533254868734
4	13.29019084431684	15.390111848396787	34.27737695620488	37.042320351081486
5	14.983705213038935	35.96088389199327	33.22753142381357	15.827879471154219
6	35.807334258185435	33.883245394809705	16.04394297291252	14.265477374092333
7	31.46182468860623	29.724087582432833	19.454794787392032	19.359292941568903
8	28.4602826958068	33.35044264415995	18.355446147905464	19.833828512127784
9	27.179066822848384	14.679012952222356	17.984040503815333	40.15787972111393
10	16.234896818268794	26.956625920326566	31.351524669320263	25.456952592084374
11	37.055578109390986	21.271700165576757	21.689951706061176	19.98277001897108
12	24.64964699656176	22.97750276464053	28.637926472198018	23.734923766599696
13	29.992190914050926	19.544047640595867	24.68744262778328	25.776318817569926
14	23.61485691530762	18.56187838684626	25.14327431698511	32.67999038086102
15	25.27359813547299	26.679147413304925	22.074803458408	25.97245099281409
16	25.757468668654866	25.833965419159092	23.173990242893343	25.234575669292695
17	24.471270579656558	25.842989104342664	24.881980232497003	24.803760083503782
18	24.860054586145566	25.417681411878885	25.314206490433204	24.408057511542346
19	25.668706855015618	24.77725573549781	25.313419302233175	24.2406181072534
20	25.88146616461965	24.53620483311476	25.175801931332416	24.406527070933176
21	25.808942868692718	24.27475053590499	25.04660886563976	24.869697729762528
22	25.82342329296915	24.093357363942193	24.93990192960273	25.143317413485928
23	25.231762307180784	24.266184790117794	25.030329175524795	25.47172372717662
24	24.959952214167412	24.35495806026066	25.230859270119677	25.454230455452254
25	25.38158768576232	24.187985455774097	25.26333073461097	25.167096123852605
26	25.114906788130632	24.444665674578804	25.189679700425664	25.2507478368649
27	25.084221590808696	24.84512148293783	24.927608434355427	25.14304849189805
28	25.327333635300704	24.49405022283382	24.928641892413133	25.249974249452343
29	25.22742121494731	24.82382074536581	24.940828249360877	25.007929790326
30	25.393400889437466	24.68975491985452	24.8344749030639	25.082369287644113
31	25.240005447421183	24.407636043310447	24.910703665459387	25.441654843808987
32	25.15879042594363	24.734717202633735	24.86435259447481	25.242139776947823
33	25.05004587628444	24.640068333900054	24.984108144775124	25.325777645040382
34	25.358845488497895	24.489583072956844	25.01148529666543	25.14008614187983
35	25.267005291406612	24.72472015190707	25.036222733827334	24.97205182285898
36	25.336594880732434	24.67682969663041	24.887442568451338	25.099132854185818
37	25.530774193576196	24.34728231287093	24.86578458215337	25.256158911399506
38	25.23563011824818	24.64835410153708	24.884307443541427	25.231708336673314
39	25.302001332555292	24.65744451062209	24.83457265572408	25.20598150109854
40	25.59867504117871	24.515789265006415	24.83315189706487	25.052383796750004
41	25.183215815193478	24.594770360173957	25.005139308664937	25.21687451596763
42	25.33952174960532	24.539823096572825	24.938487205468036	25.18216794835382
43	25.37574404280894	24.296599135529032	24.946353064286246	25.38130375737578
44	25.138657655122245	24.581311848597778	25.06151189316131	25.21851860311867
45	25.361705795524337	24.57086830508657	24.737913869824272	25.32951202956482
46	25.361523098949196	24.50573670378508	24.856808667072933	25.275931530192793
47	25.346273340777614	24.7629128204421	24.83458307349455	25.056230765285743
48	25.284964625081024	24.954919389317187	24.683703057551476	25.076412928050313
49	25.405814490714885	24.66715109263052	24.665211664839294	25.261822751815306
50	25.309587449457972	24.91925773139006	24.671923928586512	25.09923089056546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	253.0
1	176.5
2	100.0
3	258.0
4	416.0
5	439.5
6	463.0
7	357.0
8	251.0
9	368.0
10	485.0
11	797.5
12	1110.0
13	2029.5
14	2949.0
15	3763.0
16	4577.0
17	4286.5
18	3996.0
19	3693.0
20	3390.0
21	3846.0
22	4302.0
23	5569.0
24	6836.0
25	8610.5
26	10385.0
27	12742.5
28	15100.0
29	18173.0
30	21246.0
31	26159.0
32	31072.0
33	36443.0
34	41814.0
35	48645.5
36	55477.0
37	62804.0
38	70131.0
39	80270.5
40	90410.0
41	100952.5
42	111495.0
43	121964.0
44	132433.0
45	144745.5
46	157058.0
47	164676.5
48	172295.0
49	178241.5
50	184188.0
51	189287.0
52	194386.0
53	193328.5
54	192271.0
55	186294.0
56	180317.0
57	171567.5
58	162818.0
59	150708.5
60	138599.0
61	122674.0
62	106749.0
63	92672.0
64	78595.0
65	65958.5
66	53322.0
67	43943.0
68	34564.0
69	28615.5
70	22667.0
71	17907.0
72	13147.0
73	10913.0
74	8679.0
75	6952.0
76	5225.0
77	4233.0
78	3241.0
79	2509.5
80	1778.0
81	1352.5
82	927.0
83	691.5
84	456.0
85	336.5
86	217.0
87	143.0
88	69.0
89	50.5
90	32.0
91	30.0
92	28.0
93	23.5
94	19.0
95	17.5
96	16.0
97	16.0
98	16.0
99	10.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03271024412443856
2	0.004180360579803083
3	3.016755057589854E-4
4	4.3096500822712195E-5
5	0.0
6	1.292895024681366E-4
7	0.0
8	0.0
9	0.0
10	8.619300164542439E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.033510115179708E-4
17	0.0
18	2.1548250411356103E-4
19	0.0
20	3.016755057589854E-4
21	0.0
22	0.0
23	4.3096500822712195E-5
24	9.050265172769563E-4
25	1.292895024681366E-4
26	6.46447512340683E-4
27	3.016755057589854E-4
28	0.001508377528794927
29	4.3096500822712206E-4
30	0.001335991525504078
31	4.3096500822712206E-4
32	8.619300164542441E-4
33	1.292895024681366E-4
34	6.895440131633951E-4
35	3.4477200658169756E-4
36	1.292895024681366E-4
37	0.0015945705304403514
38	0.0
39	8.619300164542441E-4
40	0.0
41	9.912195189223805E-4
42	0.005387062602839025
43	0.004783711591321054
44	0.0039217815748668105
45	0.002111728540312898
46	0.0022410180427810344
47	0.001077412520567805
48	0.0037062990707532498
49	0.0043958430839166444
50	0.0012067020230359417
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2320374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.65784545764887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.56168450294835	32.38748157945242
2	16.57917333079032	16.134137074903382
3	6.767481951710885	9.878732728313286
4	3.329287404071372	6.479838079656072
5	1.851311018354476	4.5040402712567325
6	1.135589913496096	3.3153215108494734
7	0.7785375803045731	2.651737288580227
8	0.5296790576136936	2.0618433382016157
9	0.40295691848705373	1.7646313919250125
>10	1.9655143791656913	16.793277791273756
>50	0.08082252887121841	2.6380567951503773
>100	0.017693928110101922	1.2253909676723491
>500	1.7832405075521356E-4	0.08277728354289775
>1k	8.916202537760678E-5	0.08273389922238261
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.292895024681366E-4
2	0.0	0.0	0.0	0.0	1.292895024681366E-4
3	0.0	0.0	0.0	0.0	1.292895024681366E-4
4	0.0	0.0	0.0	0.0	1.292895024681366E-4
5	0.0	0.0	0.0	0.0	1.292895024681366E-4
6	0.0	0.0	0.0	0.0	3.016755057589854E-4
7	0.0	0.0	0.0	0.0	3.016755057589854E-4
8	0.0	0.0	0.0	0.0	3.016755057589854E-4
9	0.0	0.0	0.0	0.0	3.016755057589854E-4
10	0.0	0.0	0.0	0.0	3.878685074044098E-4
11	0.0	0.0	0.0	0.0	4.30965008227122E-4
12	0.0	0.0	0.0	0.0	0.0013790880263267905
13	0.0	0.0	0.0	0.0	0.0018531495353766247
14	0.0	0.0	0.0	0.0	0.0018531495353766247
15	0.0	0.0	0.0	1.292895024681366E-4	0.0023272110444264586
16	0.0	0.0	0.0	1.723860032908488E-4	0.002628886550185444
17	0.0	0.0	0.0	2.15482504113561E-4	0.002758176052653581
18	0.0	0.0	0.0	2.585790049362732E-4	0.0029305620559444295
19	0.0	0.0	0.0	3.016755057589854E-4	0.0029305620559444295
20	0.0	0.0	0.0	4.740615090498342E-4	0.003447720065816976
21	0.0	0.0	0.0	6.46447512340683E-4	0.003620106069107825
22	0.0	0.0	0.0	9.050265172769563E-4	0.0037062990707532493
23	0.0	0.0	0.0	0.0012067020230359417	0.0037062990707532493
24	0.0	0.0	0.0	0.0013790880263267905	0.0037062990707532493
25	0.0	0.0	0.0	0.0016376670312630635	0.0037062990707532493
26	0.0	0.0	0.0	0.0018962460361993368	0.003878685074044098
27	0.0	0.0	0.0	0.0022410180427810344	0.0039217815748668105
28	0.0	0.0	0.0	0.002801272553476293	0.003964878075689522
29	0.0	0.0	0.0	0.0035339130674624003	0.003964878075689522
30	0.0	0.0	0.0	0.0054301591036617376	0.003964878075689522
31	0.0	0.0	0.0	0.009222651176060411	0.004094167578157659
32	0.0	0.0	0.0	0.015557836796999104	0.004137264078980372
33	0.0	0.0	0.0	0.021117285403128978	0.004137264078980372
34	0.0	0.0	0.0	0.02667673400925885	0.004482036085562069
35	0.0	0.0	0.0	0.03378765664500637	0.004482036085562069
36	0.0	0.0	0.0	0.04369985183423017	0.004482036085562069
37	0.0	0.0	0.0	0.059214592130406564	0.004482036085562069
38	0.0	0.0	0.0	0.08003020202777655	0.004611325588030206
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2460	0.0	25.85742	1
GGTATCA	1260	0.0	25.154425	1
GTATAGG	320	0.0	17.883223	1
TATCAAC	3755	0.0	16.873262	2
TCAACGC	3730	0.0	16.75007	4
CAACGCA	3770	0.0	16.572351	5
GTATTAA	400	0.0	16.507591	1
ATCAACG	3810	0.0	16.398363	3
AACGCAG	3865	0.0	16.16501	6
AATATAG	180	5.2750693E-9	15.888322	5
TAGACAG	525	0.0	15.504207	5
TATGCCG	190	1.078115E-8	15.052094	5
GTATTAC	250	9.094947E-12	14.966884	1
GTCTTAT	280	0.0	14.93544	1
TTATACC	210	2.5484042E-9	14.666142	4
TAATACA	410	0.0	14.487287	4
GTATCGA	140	2.7597214E-5	14.142351	9
TAGGACG	125	1.6725744E-4	14.079496	4
CTTACAC	490	0.0	13.91787	3
TCGCGTG	95	0.0062382487	13.896037	44
>>END_MODULE