FastQCFastQC Report
Thu 2 Feb 2017
SRR4062164_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062164_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4469965
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74020.16559413776170506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.014.2924551
TCGCGTA2150.014.1419629
GTATCAA45700.012.9448651
CGCGTAA2400.012.27266410
GCGTAAC2650.011.83195411
CGTTATT2100.011.7583892
GCGTTAT1900.011.4947611
GTCCTAC5550.010.9499651
GACGGAC5650.010.930597
AAGACGG6100.010.90241055
ACGGACC5700.010.8348288
CGGACCA5600.010.5196619
CCGACCA4150.010.3029739
CAAGACG5550.010.2701914
CGAAAGC5550.010.26915619
AGACGGA6600.010.0770366
ATACCGT4250.010.06016
TATACCG1054.1056977E-59.9530865
ACGTTCG1151.025973E-59.91437
CGTCGTA3750.09.88147410
Produced by FastQC (version 0.11.5)